
Mass Spectrometry for the Clinical Laboratory
Description
Key Features
- Provides readers with the keys to choosing, installing, and validating a mass spectrometry platform
- Offers tools to evaluate, validate, and troubleshoot the most common assays seen in clinical pathology labs
- Explains validation, ion suppression, interference testing, and quality control design to the detail that is required for implementation in the lab
Readership
Residents and fellows in clinical pathology and clinical chemistry, practicing clinical pathologists and clinical chemists
Table of Contents
- List of Contributors
- Preface
- Chapter 1: Mass spectrometry in the clinical laboratory: determining the need and avoiding pitfalls
- Abstract
- 1. Clinical mass spectrometry
- Chapter 2: Application specific implementation of mass spectrometry platform in clinical laboratories
- Abstract
- 1. Mass spectrometry versus automated immunoassay platforms
- 2. Early decisions in the implementation of mass spectrometry in the laboratory
- 3. Importance of internal standards in mass spectrometry
- 4. Matrix effects, extraction efficiency, and ion suppression/enhancement
- 5. Analyte extraction
- 6. Choice of separation technique: GC versus LC
- 7. Choice of stationary phase
- 8. Choice of ionization techniques
- 9. Choice of mass analyzer
- 10. Summary
- Chapter 3: Sample preparation techniques for mass spectrometry in the clinical laboratory
- Abstract
- 1. Why is sample preparation needed for LC-MS/MS?
- 2. Selecting a sample preparation protocol
- 3. Description of commonly used clinical sample preparation protocols
- 4. Evaluation of sample preparation protocols
- 5. Comparison of sample preparation protocols
- 6. Optimization of sample preparation protocols
- 7. Summary
- Chapter 4: Validation, quality control, and compliance practice for mass spectrometry assays in the clinical laboratory
- Abstract
- 1. Introduction
- 2. Elements of assay validation according to CLIA, CAP, and FDA guidelines
- 3. Elements of quality control
- 4. Regulatory compliance
- 5. Additional resources from the literature
- 6. Summary
- Chapter 5: Best practices for routine operation of clinical mass spectrometry assays
- Abstract
- 1. New assay implementation
- 2. System suitability standards
- 3. Verification of new materials
- 4. Sample analysis
- 5. Data review
- 6. Instrument maintenance
- 7. Quality assurance for specimen analysis
- 8. Conclusions
- Chapter 6: Toxicology: liquid chromatography mass spectrometry
- Abstract
- 1. Toxicology testing in the clinical laboratory
- 2. Chromatographic methods for toxicology testing in the clinical laboratory
- 3. Toxicology LC-MS applications
- 4. Conclusions
- Chapter 7: Toxicology: GCMS
- Abstract
- 1. Use and strengths of GC-MS in clinical toxicology
- 2. Limitations of GC-MS in clinical toxicology
- 3. Instrument selection
- 4. Defining an assay method
- 5. Analysis
- 6. Assay validation
- 7. Instrument maintenance and troubleshooting
- 8. Personnel/training
- Chapter 8: Therapeutic drug monitoring using mass spectrometry
- Abstract
- 1. Background/introduction
- 2. MS considerations for TDM
- 3. Assay design/test life cycle considerations
- 4. Sample preparation considerations
- 5. Validation considerations
- 6. Quality control/quality assurance considerations
- 7. Conclusions
- Chapter 9: Vitamin D metabolite quantitation by LC-MS/MS
- Abstract
- 1. Physiological role of vitamin D
- 2. Evolution of assays for vitamin D metabolites
- 3. Clinical utility and quantitation of vitamin D and its metabolites by LC-MS/MS
- 4. Conclusions
- Chapter 10: Steroid hormones
- Abstract
- 1. Steroid hormone applications for clinical use
- 2. Calibration and quality control requirements
- 3. Sample preparation
- 4. Instrumentation
- 5. Validation
- 6. Postimplementation Monitoring
- 7. Preanalytical considerations
- 8. Postanalytical considerations
- Disclaimer
- Chapter 11: Mass spectrometry in the clinical microbiology laboratory
- Abstract
- 1. Introduction
- 2. How MALDI-TOF MS works
- 3. Commercial systems
- 4. Bacteriology
- 5. Mycobacteriology
- 6. Mycology
- 7. Other Possible MALDI-TOF MS Applications
- 8. Other MS technologies
- 9. Summary and Conclusions
- Chapter 12: High resolution accurate mass (HRAM) mass spectrometry
- Abstract
- 1. Introduction
- 2. HRMS fundamentals
- 3. Clinical and forensic toxicology
- 4. Microbiology
- 5. Molecular diagnostics
- 6. Future HRMS clinical applications and developments
- 7. Conclusions
- Chapter 13: Evolving platforms for clinical mass spectrometry
- Abstract
- 1. Introduction
- 2. Triple quadrupole
- 3. Quadrupole-time of flight (TOF) and TOF
- 4. Ion trap and orbitrap
- 5. MALDI-TOF
- 6. Hybrid instruments
- 7. PCR-MS
- 8. Front end—sample preparation and collection/ambient ionization/real time analysis
- 9. Ambient and direct ionization approaches
- 10. Imaging MS
- 11. DESI imaging
- 12. Ion mobility mass spectrometry
- 13. Proteomics
- 14. Future implications
- Index
Product details
- No. of pages: 304
- Language: English
- Copyright: © Academic Press 2016
- Published: November 2, 2016
- Imprint: Academic Press
- Hardcover ISBN: 9780128008713
- eBook ISBN: 9780128009925
About the Editors
Hari Nair

Dr. Nair has co-authored several peer reviewed articles, a patent and served as a consulting editor for a med tech text book published by Elsevier.
Affiliations and Expertise
William Clarke

Affiliations and Expertise
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