Description

This volume presents a compilation of experimental methods and principles, with detailed protocols and examples. Techniques which have demonstrated value in problem-solving are included. Intended for biochemists, cell biologists, microbiologists, pharmacologists, biomedical scientists, biophysicists, analytical chemists, and physiologists, this book focuses on mass spectrometry. Topics discussed include the methods of ionization, liquid chromatography, continuous flow fast atom bombardment mass spectrometry, collision-induced dissociation, enzymatic and chemical digestion of proteins, and constituents of nucleic acids. Other titles in this series include Guide to Yeast Genetics and Molecular Biology and Retinoids, Part B: Cell Differentiation and Clinical Applications.

Readership

Biochemists, analytical and organic chemists, biophysicists, molecular biologists, and physical and pharmaceutical chemists.

Table of Contents

J.A. McCloskey, Introduction. General Techniques: R.J. Cotter and A.G. Harrison, Methods of Ionization. K.R. Jennings and G.G. Dolnikowski, Mass Analyzers. S. Evans, Detectors. J.T. Watson, Selected Ion Measurements. M.L. Vestal, Liquid Chromatography-Mass Spectrometry. M.L. Gross, Tandem Mass Spectrometry: Multisector Magnetic Instruments. R.A. Yost and R.K. Boyd, Tandem Mass Spectrometry: Quadrupole and Hybrid Instruments. E. De Pauw, Matrix Selection for Liquid Secondary Ion and Fast Atom Bombardment Mass Spectrometry. R.M. Caprioli, Continuous-Flow Fast Atom Bombardment Mass Spectrometry. M.L. Gross and R.N. Hayes, Collison-Induced Dissociation. R.D. Macfarlane, Principles of Californium-252 Plasma Desorption Mass Spectrometry Applied to Protein Analysis. F. Hillenkamp and M. Karas, Mass Spectrometry of Peptides and Proteins by Matrix-Assisted Ultraviolet Laser Desorption/Ionization. K. Biemann, Utility of Exact Mass Measurements. R.M. Milberg, Selection and Use of Mass Reference Standards. D.R. Knapp, Chemical Derivatization for Mass Spectrometry. J.A. McCloskey, Introduction of Deuterium by Exchange for Measurement by Mass Spectrometry. R.C. Murphy and K.L. Clay, Preparation of Labeled Molecules by Exchange with Oxygen-18 Water. Peptides and Proteins: K. Biemann, Peptides and Proteins: Overview and Strategy. T.D. Lee and J.E. Shively, Enzymatic and Chemical Digestion of Proteins for Mass Spectrometry. D.L. Smith and Z. Zhou, Strategies for Locating Disulfide Bo

Details

No. of pages:
960
Language:
English
Copyright:
© 1990
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080881140
Print ISBN:
9780121820947

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Reviews

@from:Praise for the Volume @qu:"This volume will be a most welcome addition to the shelves of those working with mass spectrometry (MS) applications... The book is useful and comprehensive, offering a concise treatment of experimental principles along with detailed descriptions of specific methods... This book is a gold mine of information. The standards of excellence established in the Methods in Enzymology series have been continued in this volume. The 70 authors and the editor are to be congratulated." @source:--SPECTROSCOPY @qu:"The Methods in Enzymology series has a well-established reputation for producing carefully edited, authoritative, practical summaries of current research by experts in various specialty fields. J.A. McCloskey has continued that high standard in his selection of 70 scientists who have written 47 chapters... occupies a niche not filled previously by any single publication in the field... There are six very useful appendices to help guarantee that this volume will find a permanent home close to the operator of mass spectrometer (elemental mass and abundance data, ions in 11 defined reference materials for different types of ionization, characteristic spectra of seven FAB matrices, calculation of isotopic abundance distributions, nomenclature for peptide fragment ions, and residue masses for peptides). In summary, this book is an extremely valuable resource for the practicing mass spectroscopist. Due to the inclusion of general introductory material, it would be an excellent text for a course in biological mass spectrometry directed toward graduate students in chemistry or biochemistry." @source:--BOOK REVIEWS @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL