Mammalian Cell Membranes

General Concepts

1st Edition - January 1, 1976
Editors: G. A. Jamieson, D. M. Robinson
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 1 - 6 2 7 7 - 5

Mammalian Cell Membranes, Volume 1: General Concepts is a collection of papers that deals on the physical and chemical studies focusing on membrane structure and function. This… Read more

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Mammalian Cell Membranes, Volume 1: General Concepts is a collection of papers that deals on the physical and chemical studies focusing on membrane structure and function. This collection reviews the interpretation of the anatomy of the mammalian cell, including its separation and cultivation. The different methods of isolation of its surface membrane are then evaluated to bring some understanding of the subject. More descriptions of the various physical techniques adopted to membrane constituents and to cell membrane research, such as nuclear magnetic resonance, electron spin resonance, fluorescence, and flash photolysis spectroscopy are given. Discoveries of mitochondrial DNA and other techniques have increased investigation of the synthesis and components of functional mitochondria, leading to different perspectives on models of membrane structure. This book can serve the needs of biochemists and microbiologists in advancing their work, research, and understanding of mammalian cell membranes.

1 A Current Interpretation of The Anatomy of The Mammalian Cell

1.1 Introduction

1.2 Membranous Components

1.3 Nonmembranous Components

2 The Separation and Cultivation of Mammalian Cells

2.1 Introduction

2.2 Cell Separation and Fractionation

2.3 Cell Cultivation

3 Isolation of Surface Membranes From Mammalian Cells

3.1 Introduction

3.2 Preparation

3.3 Isolation

3.4 Markers

3.5 Contaminants

3.6 Procedural Artifacts

3.7 Comments on Isolated Membranes

3.8 Conclusions

4 Physical Studies of Membranes

5 Physicochemical Studies of Cellular Membranes

5.1 Introduction

5.2 The Constituents of Cell Membranes

5.3 Physical Properties of the Membrane Constituents

5.4 Physicochemical Properties of Cell Membranes

6 Mechanical Properties of Cellular Membranes

6.1 Introduction

6.2 Mechanical Properties of The Red Cell Membrane

6.3 Mechanical Properties of Other Membranes

6.4 Summary and Conclusions

7 Enzyme Distribution In Mammalian Membranes

7.1 Membranes to Be Discussed As Enzyme Sites

7.2 Isolation of Membrane Fragments

7.3 Methodology for Assigning Enzymes To Particular Membranes

7.4 Methods for the Study of Membrane-Associated Enzymes

7.5 Enzymes of Particular Membrane Systems

7.6 Concluding Comments

8 Electrostatic Control of Membrane Permeability via Intramembranous Particle Aggregation

8.1 Introduction

8.2 Glycoprotein Nature of Intramembranous Particles

8.3 Mobility of Intramembranous Particles

8.4 Forces Between Intramembranous Particles

8.5 Results and Discussion

8.6 Biological Evidence

8.7 Synopsis and Conclusion

Appendix

9 Biogenesis of Mammalian Membranes

9.1 Introduction

9.2 Examples of Model Systems for Membrane Biogenesis

9.3 General Remarks on the Biogenesis of Membranes

9.4 Evidence for Mosaicism In Membranes

9.5 Role of Mitochondria In Protein Synthesis

9.6 Evidence for Division of Mitochondria In Mammalian Cells

9.7 Conclusions

10 A Perspective on Models of Membrane Structure

10.1 Introduction

10.2 Some Basic Considerations

10.3 Dynamic Structure of the Lipid Bilayer

10.4 Interactions Between Lipids and Proteins

10.5 Membrane Asymmetry

10.6 Arrangement and Motion of Membrane Proteins

Index