Male Reproductive Toxicology

Male Reproductive Toxicology

1st Edition - July 13, 1993

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  • Editors: Robert E. Chapin, Jerrold J. Heindel
  • eBook ISBN: 9781483270951

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Methods in Toxicology, Volume 3: Male Reproductive Toxicology, Part A, deals with the male reproductive system and discusses methods that will help identify toxicant-induced changes at all levels in living organisms. It is important to realize that a toxic effect does not occur in a vacuum. All work in toxicology must be predicated on a demonstrated adverse effect in vivo. If good toxicology cannot exist in a vacuum, then there must be a structure. Thus, the book begins by presenting a few models as examples of the ways experiments could be grouped to define the toxicity of a chemical. This is followed by separate chapters on methods such as male mouse sexual behavior test; in vitro techniques for assessing pituitary secretory function; histological methods for preservation of the rat testis; procedures for assessing testicular sperm head counts in mice, rats, and dogs; and guidelines for conducting rodent dominant lethal tests. Subsequent chapters cover topics such as methods for the isolation and purification of Leydig cells from rat and mouse testes, and techniques used in semen analysis and fertility assessment in the rabbit.

Table of Contents

  • Contributors

    1. Introduction

    2. Male Mouse Sexual Behavior Test

    3. In Vitro Techniques for Assessing Pituitary Secretory Function

    4. Histological Methods for Evaluation of the Testis

    5. Application of Testicular Sperm Head Counts in the Assessment of Male Reproductive Toxicity

    6. Stage Synchronization in Rat Seminiferous Tubules Using Vitamin A Depletion and Repletion

    7. Spermatogonial Stem Cells: Assessing Their Survival and Ability to Produce Differentiated Cells

    8. Dominant Lethal Tests in Male and Female Mice

    9. Transillumination-Phase-Contrast Microscopic Techniques for Evaluation of Male Germ Cell Toxicity and Mutagenicity

    10. Isolation and Culture of Leydig Cells from Adult Rats

    11. Isolation and Primary Culture of Leydig Cells

    12. Purification and Primary Culture of Leydig Cells

    13. Preparation and Use of Sertoli Cell-Enriched Cultures from 18-Day-Old Rats

    14. Two-Compartment Cultures of Sertoli Cells-Applications in Testicular Toxicology

    15. Isolation, Separation, and Short-Term Culture of Spermatogenic Cells

    16. Collection of Interstitial Fluid and Seminiferous Tubule Fluid from the Rat Testis

    17. Isolation and Culture of Epididymal Epithelial Cells from Adult Rats

    18. Evaluation of Protein Synthesis by the Epididymis

    19. Utilizing Cryo Resources CellSoft Computer-Assisted Sperm Analysis System for Rat Sperm Motility Studies

    20. Computer-Assisted Sperm Analysis of Rodent Epididymal Sperm Motility Using the Hamilton-Thorn Motility Analyzer

    21. Methods for Evaluation of Rat Epididymal Sperm Morphology

    22. Semen Analysis and Fertility Assessment in the Rabbit

    23· General Techniques for Assessing Male Reproductive Potential in Human Field Studies


Product details

  • No. of pages: 402
  • Language: English
  • Copyright: © Academic Press 1993
  • Published: July 13, 1993
  • Imprint: Academic Press
  • eBook ISBN: 9781483270951

About the Editors

Robert E. Chapin

Jerrold J. Heindel

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