Macromolecular Crystallography, Part A

Macromolecular Crystallography, Part A

1st Edition - February 12, 1997
This is the Latest Edition
  • Editors: Charles Carter, Jr., Robert Sweet
  • Hardcover ISBN: 9780121821777

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

  • Crystal Growth
  • Phase Determination
  • Instrumentation and Data Collection
  • Model Building
  • Refinement


Biochemists, biophysicists, analytical chemists, physical chemists.

Table of Contents

  • Introduction: B.W. Matthews, Recent Transformations in Structural Biology. Crystals: P.C. Weber, Overview of Protein Crystallization Methods. M. Rigs-Kautt and A. Ducruix, Inferences Drawn from Physicochemical Studies of Crystallogenesis and Precrystalline State. L. Song and J.E. Gouaux, Membrane Protein Crystallization: Application of Sparse Matrix to alpha-Hemolysin Heptamer. C.W. Carter, Jr., Response Surface Methods for Optimizing and Improving Reproducibility of Crystal Growth. A. George, Y. Chiang, B. Guo, A. Arabshahi, Z. Cai, and W.W. Wilson, Second Virial Coefficient as Predictor in Protein Crystal Growth. J.R. Luft and G.T. DeTitta, Kinetic Aspects of Macromolecular Crystallization. R. Sousa, Using Cosolvents to Stabilize Protein Conformation for Crystallization. C.E. Kundrot, Preparation and Crystallization of RNA: A Sparse Matrix Approach. A.R. Ferre-Damare and S.K. Burley, Dynamic Light Scattering in Evaluating Crystallizability of Macromolecules. A.M. Edwards, S.A. Darst, S.A. Hemming, F.J. Asturias, P.R. David, and R.D. Kornberg, Two-Dimensional Protein Crystals in Aid of Three-Dimensional Protein Crystal Growth. I. Rayment, Reductive Alkylation of Lysine Residues to Alter Crystallization Properties of Proteins. Data: Cryocrystallography: D. Rodgers, Practical Cryocrystallography. J.R. Helliwell, Overview of Synchrotron Radiation and Macromolecular Crystallography. Z.R. Korszun, Neutron Macromolecular Crystallography. Detectors:Y. Amemiya, X-Ray Storage-Phosphor Imaging-Plate Detectors: High-Sensitivity X-Ray Area Detector. E.M. Westbrook and I. Naday, Charge-Coupled Device-Based Area Detectors. R. Kahn and R. Fourme, Gas Proportional Detectors. Data Collection Software:J.W. Pflugrath, Diffraction-Data Processing for Electronic Detectors: Theory and Practice. Z. Otwinowski and W. Minor, Processing of X-Ray Diffraction Data Collected in Oscillation Mode. Z. Dauter, Data Collection Strategy. T.O. Yeates, Detecting and Overcoming Crystal Twinning. Phases: Basic Concepts:G. Bricogne, Bayesian Statistical Viewpoint on Structure Determination: Basic Concepts and Examples. G. Bricogne, Efficient Sampling Methods for Combinations of Signs, Phases, Hyperphases, and Molecular Orientations. H. Ke, Overview of Isomorphous Replacement Phasing. M.A. Rould, Screening for Heavy-Atom Derivatives and Obtaining Accurate Isomorphous Differences. E. de La Fortelle and G. Bricogne, Maximum-Likelihood Heavy-Atom Parameter Refinement for Multiple Isomorphous Replacement and Multiwavelength Anomalous Diffraction Methods. Multiwavelength Methods:W.A. Hendrickson and C.M. Ogata, Phase Determination by Multiwavelength Anomalous Diffraction Methods. S. Doublie, Preparation of Selenomethionyl Proteins for Phase Determination. T.C. Terwilliger, Multiwavelength Anomalous Diffraction Phasing of Macromolecular Structures: Analysis of MAD Data as Single Isomorphous Replacement with Anomalous Scattering Data Using MADMRG Program. V. Ramakrishnan and V. Biou, Treatment of Multiwavelength Anomalous Diffraction Data as a Special Case of Multiple Isomorphous Replacement. Molecular Replacement:A.T. Branger, Patterson Correlation Searches and Refinement. J. Navaza and P. Saludjian, AMoRe: An Automated Molecular Replacement Program Package. L. Tong and M. Rossmann, Rotation Function Calculations with GLRF Program. G.A. Bentley, Phased Translation Function. Horizon MethodsJ.M. Berg and N.W. Goffeney Centrosymmetric Crystals of Biomolecules: The Racemate Method. G.M. Sheldrick, Patterson Superposition and ab Initio Phasing. A.D. Podjarny and A.G. Urzhumtsev, Low-Resolution Phasing. Author

Product details

  • No. of pages: 700
  • Language: English
  • Copyright: © Academic Press 1997
  • Published: February 12, 1997
  • Imprint: Academic Press
  • Hardcover ISBN: 9780121821777
  • About the Serial Volume Editors

    Charles Carter, Jr.

    Affiliations and Expertise

    University of North Carolina, Chapel Hill, U.S.A.

    Robert Sweet

    Affiliations and Expertise

    Brookhaven National Laboratory, Upton, New York, U.S.A.

    About the Editors in Chief

    John Abelson

    Affiliations and Expertise

    California Institute of Technology, Division of Biology, Pasadena, U.S.A.

    Melvin Simon

    Affiliations and Expertise

    The Salk Institute, La Jolla, CA, USA