Liquid Chromatography

Liquid Chromatography

Fundamentals and Instrumentation

3rd Edition - January 1, 2023

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  • Editors: Salvatore Fanali, Bezhan Chankvetadze, Paul Haddad, Colin Poole, Marja-Liisa Riekkola
  • Paperback ISBN: 9780323999687

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Liquid Chromatography: Fundamentals and Instrumentation, Third Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of the field. It helps them acquire basic and advanced theoretical concepts, recognition mechanisms, conventional and advanced instrumentation, method development, data analysis, and more. In the years since the second edition was published, numerous papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, and development of theory. This third edition addresses these new developments with updated chapters from the most expert researchers in the field. Together with Liquid Chromatography: Applications, this book is a valuable reference for scientists working in academia and research institutes, teachers and university students, and industry professionals in R&D and quality control managers.

Key Features

  • Emphasizes the integration of chromatographic methods and sample preparation
  • Provides important data related to complex matrices, sample preparation, and data handling
  • Gives background information to facilitate the choice of LC sub-technique and experimental conditions - mobile and stationary phases, detectors, data processing, and more
  • New to this edition: All chapters have been brought up to date, and all-new chapters have been added on the topics of chiral recognition, co-solvents and mobile phase additives, Physicochemical measurements, and Identification and quantitation in mass spectrometry


Scientists working in academia/research institutes; teachers and university students from B.Sc. level to PhD level; industry professionals in R&D and quality control managers

Table of Contents

  • 1. Milestones in the development of liquid chromatography
    2. Kinetic theories of liquid chromatography
    3. Column technology in liquid chromatography
    4. General instrumentation in HPLC
    5. Liquid-solid chromatography
    6. Reversed-phase liquid chromatography
    7. Secondary chemical equilibria in reversed-phase liquid chromatography
    8. Ultra-high performance and Ultrafast liquid chromatography
    9. Nano-liquid chromatography
    10. Hydrophilic interaction liquid chromatography
    11. Mobile phase selection in liquid chromatography
    12. Co-solvents and mobile phase additives (new chapter)
    13. Method development in liquid chromatography
    14. Physicochemical measurements
    15. Theory and practice of gradient elution liquid chromatography
    16. Fundamentals of Enantioselective LC (Chiral recognition mechanisms)
    17. Hydrophobic interaction chromatography
    18. Ion chromatography
    19. Size-exclusion chromatography
    20. Interaction polymer chromatography
    21. Affinity chromatography
    22. Multidimensional Liquid Chromatography
    23. Process concepts in preparative chromatography
    24. Modeling of preparative liquid chromatography
    25. Capillary electrochromatography
    26. Liquid Chromatography on microchip
    27. Mass spectrometric detection, instrumentation and ionization methods
    28. Identification and quantitation in Mass Spectrometry
    29. Advanced IR and Raman detectors for identification and quantification
    30. Advanced spectroscopic detectors for identification and quantification: Nuclear magnetic resonance
    31. Prediction of retention in Liquid Chromatography
    32. Validation of liquid chromatographic methods – chemometrics and calibration

Product details

  • No. of pages: 1016
  • Language: English
  • Copyright: © Elsevier 2023
  • Published: January 1, 2023
  • Imprint: Elsevier
  • Paperback ISBN: 9780323999687

About the Editors

Salvatore Fanali

Salvatore Fanali
Salvatore Fanali is Director of Research at the Institute of Chemical Methodologies, Italian National Research Council (C.N.R.) in Monterotondo (Rome), Italy, and head of the Capillary Electromigration and Chromatographic Methods Unit at the same Institute. His research activity is mainly focused on separation science including the development of modern miniaturized techniques (electrodriven and liquid chromatography). He also studies hyphenation with mass spectrometry and development of new stationary phases. Separation methods developed are currently applied to food, pharmaceuticals, chiral environment, and biomedical analysis. He is Editor of the Journal of Chromatography A and a member of the advisory editorial board of seven international scientific journals. Fanali is the author of about 300 publications including some book chapters. He received several awards including the “Liberti Medal” in Separation Science from the Italian Chemical Society.

Affiliations and Expertise

Director and Head, Capillary Electromigration and Chromatographic Methods Unit, Institute of Chemical Methodologies, Italian National Research Council (C.N.R.), Monterotondo, Rome, Italy

Bezhan Chankvetadze

Bezhan Chankvetadze is professor of Analytical Chemistry at Tbilisi State University, Editor of Journal of Pharmaceutical and Biomedical Analysis, and is well recognized in the field of separation science. He is one of the leaders in chromatography and chiral separations with a large number of publications.

Affiliations and Expertise

Professor, Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia

Paul Haddad

Paul Haddad
Paul Haddad is currently a Distinguished Professor of Chemistry and Australian Research Council Federation Fellow at the University of Tasmania, as well as Director of the Pfizer Analytical Research Centre. He has more than 500 publications in this field and has presented in excess of 450 papers at local and international scientific meetings. He is an editor of Journal of Chromatography A, a contributing editor for Trends in Analytical Chemistry, and was an editor of Analytica Chimica Acta for 6 years. He is currently a member of the editorial boards of 10 other journals of analytical chemistry or separation science. He is the recipient of several national and international awards, including the ACS Award in Chromatography, the Marcel Golay Award, the AJP Martin Gold Medal awarded by the Chromatographic Society, the Royal Society of Chemistry Analytical Separation Methods Award, the RACI HG Smith and Analytical Division medals, and more.

Affiliations and Expertise

Distinguished Professor, School of Chemistry, University of Tasmania, Hobart, Tasmania, Australia

Colin Poole

Colin Poole
Professor Colin Poole is internationally known in the field of thin-layer chromatography and is an editor of the Journal of Chromatography and former editor of the Journal of Planar Chromatography – Modern TLC. He has authored several books on chromatography, recent examples being The Essence of Chromatography published by Elsevier (2003), and Gas Chromatography published by Elsevier (2012). He is the author of approximately 400 research articles, many of which deal with thin-layer chromatography, and is co-chair of the biennial “International Symposium on High-Performance Thin-Layer Chromatography”.

Affiliations and Expertise

Department of Chemistry, Wayne State University, Detroit, MI, USA

Marja-Liisa Riekkola

Marja-Liisa Riekkola is a professor of Analytical Chemistry at Helsinki University, Helsinki, Finland. She is well recognized in the field of separation science. She serves as Editor of Journal of Chromatography A. Prof. Riekkola is one of the leaders in chromatography with a large number of publications.

Affiliations and Expertise

Professor, Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Finland

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