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Liquid Chromatography - 2nd Edition - ISBN: 9780128053935, 9780128093450

Liquid Chromatography

2nd Edition

Fundamentals and Instrumentation

Editors: Salvatore Fanali Paul R. Haddad Colin Poole Marja-Liisa Riekkola
eBook ISBN: 9780128093450
Paperback ISBN: 9780128053935
Imprint: Elsevier
Published Date: 21st June 2017
Page Count: 808
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Table of Contents

  1. Milestones in the development of liquid chromatography
    2. Kinetic theories of liquid chromatography
    3. Column technology in liquid chromatography
    4. Reversed-phase liquid chromatography
    5. Secondary chemical equilibria in reversed-phase liquid chromatography
    6. Hydrophilic interaction chromatography
    7. Hydrophobic interaction chromatography
    8. Liquid-solid chromatography
    9. Ion chromatography
    10. Size-exclusion chromatography
    11. Interaction polymer chromatography
    12. Affinity chromatography
    13. Solvent selection in liquid chromatography
    14. Method development in liquid chromatography
    15. Theory and practice of gradient elution liquid chromatography
    16. Comprehensive two-dimensional liquid chromatography
    17. General instrumentation in HPLC
    18. Advanced spectrometric detectors for identification and quantification: Mass spectrometry
    19. Advanced IR and Raman detectors for identification and quantification
    20. Advanced spectrometric detectors for identification and quantification: Nuclear magnetic resonance
    21. Data analysis
    22. Validation of liquid chromatographic methods
    23. Quantitative structure property (retention) relationships in liquid chromatography
    24. Modeling of preparative liquid chromatography
    25. Process concepts in preparative liquid chromatography
    26. Miniaturization and microfluidics
    27. Nano-liquid chromatography
    28. Capillary electrochromatography
    29. Ultra-high performance liquid chromatography


Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field.

In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more.

This second edition addresses these new developments with updated chapters from the most expert researchers in the field.

Key Features

  • Emphasizes the integration of chromatographic methods and sample preparation
  • Explains how liquid chromatography is used in different industrial sectors
  • Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical)
  • Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making


Scientists working in academia/research institutes; teachers and university students from B.Sc. level to PhD level; industry professionals in R&D and quality control managers


No. of pages:
© Elsevier 2017
21st June 2017
eBook ISBN:
Paperback ISBN:


"The second edition of Liquid chromatography: fundamentals and instrumentation is a valuable resource for an analytical chemist. It contains in-depth explanations of separation mechanisms and detectors that will aid in method development and the optimization of selectivity and sensitivity required for good quantitative and qualitative determinations." --Analytical and Bioanalytical Chemistry

Ratings and Reviews

About the Editors

Salvatore Fanali

Salvatore Fanali

Salvatore Fanali is Director of Research at the Institute of Chemical Methodologies, Italian National Research Council (C.N.R.) in Monterotondo (Rome), Italy, and head of the Capillary Electromigration and Chromatographic Methods Unit at the same Institute. His research activity is mainly focused on separation science including the development of modern miniaturized techniques (electrodriven and liquid chromatography). He also studies hyphenation with mass spectrometry and development of new stationary phases. Separation methods developed are currently applied to food, pharmaceuticals, chiral environment, and biomedical analysis. He is Editor of the Journal of Chromatography A and a member of the advisory editorial board of seven international scientific journals. Fanali is the author of about 300 publications including some book chapters. He received several awards including the “Liberti Medal” in Separation Science from the Italian Chemical Society.

Affiliations and Expertise

Director, Institute of Chemical Methodologies, Italian National Research Council (C.N.R.), Monterotondo, Rome, Italy Head, Capillary Electromigration and Chromatographic Methods Unit, Institute of Chemical Methodologies, Italian National Research Council (C.N.R.), Monterotondo, Rome, Italy

Paul R. Haddad

Paul R. Haddad

Paul Haddad is currently a Distinguished Professor of Chemistry and Australian Research Council Federation Fellow at the University of Tasmania, as well as Director of the Pfizer Analytical Research Centre. He has more than 500 publications in this field and has presented in excess of 450 papers at local and international scientific meetings. He is an editor of Journal of Chromatography A, a contributing editor for Trends in Analytical Chemistry, and was an editor of Analytica Chimica Acta for 6 years. He is currently a member of the editorial boards of 10 other journals of analytical chemistry or separation science. He is the recipient of several national and international awards, including the ACS Award in Chromatography, the Marcel Golay Award, the AJP Martin Gold Medal awarded by the Chromatographic Society, the Royal Society of Chemistry Analytical Separation Methods Award, the RACI HG Smith and Analytical Division medals, and more.

Affiliations and Expertise

Distinguished Professor, School of Chemistry, University of Tasmania, Hobart, Tasmania, Australia

Colin Poole

Colin Poole

Professor Colin Poole is internationally known in the field of thin-layer chromatography and is an editor of the Journal of Chromatography and former editor of the Journal of Planar Chromatography – Modern TLC. He has authored several books on chromatography, recent examples being The Essence of Chromatography published by Elsevier (2003), and Gas Chromatography published by Elsevier (2012). He is the author of approximately 400 research articles, many of which deal with thin-layer chromatography, and is co-chair of the biennial “International Symposium on High-Performance Thin-Layer Chromatography”.

Affiliations and Expertise

Department of Chemistry, Wayne State University, Detroit, MI, USA

Marja-Liisa Riekkola

Marja-Liisa Riekkola is a professor of Analytical Chemistry at Helsinki University, Helsinki, Finland. She is well recognized in the field of separation science. She serves as Editor of Journal of Chromatography A. Prof. Riekkola is one of the leaders in chromatography with a large number of publications.

Affiliations and Expertise

Professor, Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Finland