Liquid Chromatography

1st Edition


Editors: Salvatore Fanali Paul R. Haddad Colin Poole David Lloyd
Hardcover ISBN: 9780124158061
eBook ISBN: 9780124158665
Imprint: Elsevier
Published Date: 25th January 2013
Page Count: 688
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A single source of authoritative information on all aspects of the practice of modern liquid chromatography suitable for advanced students and professionals working in a laboratory or managerial capacity

Key Features

  • Chapters written by authoritative and visionary experts in the field provide an overview and focused treatment of a single topic
  • Each chapter emphasizes the integration of chromatographic methods and sample preparation, automation, and explains how liquid chromatography is used in different industrial sectors
  • Focuses on expanding and illustrating the main features of the fundamental section, while demonstrating where and how the best practices of liquid chromatography are utilized
  • Comprehensive coverage of modern liquid chromatography from theory, to methods, to selected applications
  • Thorough selected references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision making


Practitioners of distillation and separation science looking for a quick access to the newest knowledge; graduate students searching for special applications; chemists; professional scientists in academia, industry and government laboratories; environmental engineers; mechanical engineers

70% professionals, 20% students, 10% others.

Table of Contents


Chapter 1. Affinity Chromatography

1.1 Introduction

1.2 Basic Components of Affinity Chromatography

1.3 Bioaffinity Chromatography

1.4 Immunoaffinity Chromatography

1.5 Dye-Ligand and Biomimetic Affinity Chromatography

1.6 Immobilized Metal-Ion Affinity Chromatography

1.7 Analytical Affinity Chromatography

1.8 Miscellaneous Methods and Newer Developments



Chapter 2. Derivatization in Liquid Chromatography

2.1 Introduction

2.2 Reagent Selection

2.3 Postcolumn Reaction Detectors

2.4 Conclusions


Chapter 3. Validation of Liquid Chromatographic Methods

3.1 Traditional Method Validation

3.2 Quality by Design and Analytical Methods

3.3 Conclusion


Chapter 4. Liquid Chromatographic Separation of Enantiomers

4.1 Introduction

4.2 A Short History of Chiral Separations by Liquid Chromatography

4.3 Materials for the Liquid Chromatographic Separation of Enantiomers

4.4 Modes of Liquid Chromatographic Separation of Enantiomers

4.5 Separation of Enantiomers by Supercritical Fluid Chromatography

4.6 Summary and Future Trends


Chapter 5. Liquid Interaction Chromatography of Polymers

5.1 Introduction

5.2 Theoretical Aspects of Isocratic Liquid Chromatography of Polymers

5.3 Applications of Liquid Chromatography of Polymers

5.4 Hyphenated Techniques

5.5 Summary


Chapter 6. Amino Acid and Bioamine Separations

6.1 Introduction

6.2 Direct Separation of Amino Acids and Amines

6.3 Indirect Separation of Amino Acids and Amines

6.4 Enantioselective Liquid Chromatographic Analysis of Amino Acids

6.5 Conclusions




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About the Editor

Salvatore Fanali

Salvatore Fanali is Director of Research at the Institute of Chemical Methodologies, Italian National Research Council (C.N.R.) in Monterotondo (Rome), Italy, and head of the Capillary Electromigration and Chromatographic Methods Unit at the same Institute. His research activity is mainly focused on separation science including the development of modern miniaturized techniques (electrodriven and liquid chromatography). He also studies hyphenation with mass spectrometry, and development of new stationary phases. Separation methods developed are currently applied to food, pharmaceuticals, chiral environment, and biomedical analysis. He is Editor of the Journal of Chromatography A and a member of the advisory editorial board of seven international scientific journals. Fanali is the author of about 300 publications including some book chapters. He received several awards including the “Liberti Medal” in Separation Science from the Italian Chemical Society.

Affiliations and Expertise

Istituto di Metodologie, CNR, Rome, Italy

Paul R. Haddad

Paul Haddad has obtained the degrees of BSc, PhD, and DSc from the University of New South Wales. His academic career has been spent at the Australian National University, the University of New South Wales, and since 1992 at the University of Tasmania where he is currently a Distinguished Professor of Chemistry and Australian Research Council Federation Fellow. He was Director of the Australian Centre for Research on Separation Science from 2001-2013 and is currently Director of the Pfizer Analytical Research Centre. He has a long-standing research interest in analytical separations of inorganic species and has more than 500 publications in this general field. He has also presented in excess of 450 papers at local and international scientific meetings. He is an editor of Journal of Chromatography A (since 2005), a contributing editor for Trends in Analytical Chemistry (since 2000), and was an editor of Analytica Chimica Acta for 6 years. He is currently a member of the editorial boards of 10 other journals of analytical chemistry or separation science. He is the recipient of a number of national and international awards, including the ACS Award in Chromatography, the Marcel Golay Award, the AJP Martin Gold Medal awarded by the Chromatographic Society, the Royal Society of Chemistry Analytical Separation Methods Award, the RACI HG Smith and Analytical Division medals, the inaugural University of Tasmania Vice-Chancellor's Medal for Research Excellence, and the inaugural Tasmanian Premier’s Scientist of the Year award.

Affiliations and Expertise

School of Chemistry, Univ. of Tasmania, Hobart, Australia

Colin Poole

Professor Colin Poole is internationally known in the field of thin-layer chromatography and is an editor of the Journal of Chromatography and former editor of the Journal of Planar Chromatography – Modern TLC. He has authored several books on chromatography, recent examples being “The Essence of Chromatography” published by Elsevier (2003), and “Gas Chromatography” published by Elsevier (2012). He is the author of approximately 400 research articles, many of which deal with thin-layer chromatography, and is co-chair of the biennial “International Symposium on High-Performance Thin-Layer Chromatography”.

Affiliations and Expertise

Wayne State University, Detroit, MI, USA

David Lloyd

David Lloyd obtained his PhD from the Department of Chemistry, University of York, UK, working with Prof. David Goodall on the development of laser-based polarimetry as a chiral detection method. He then completed postdoctoral research on bioanalytical capillary electrophoresis (CE) with Prof. Irving Wainer at St. Jude Children’s Research Hospital in Memphis, TN. He has since worked both in academia (McGill University, Departments of Oncology and Experimental Medicine) and the pharmaceutical industry (DuPont Pharmaceuticals and Bristol-Myers Squibb). Whilst at McGill, Dr. Lloyd’s analytical research focused on microscale bioanalysis by CE and on chiral CE. Since moving to the pharmaceutical industry, his responsibilities have been in analytical R&D for projects ranging from the late discovery stage through worldwide registrational filings, with a primary focus on separations science. From 1995 – 2002, Dr. Lloyd was Contributing Editor of TrAC - Trends in Analytical Chemistry; and from 1999 - 2011, he was Editor of the Journal of Chromatography B.

Affiliations and Expertise

Bristol-Myers Squibb, New Brunswick, NJ, USA


"...provides invaluable information for laboratory scientists who are looking for information on how to separate different types of molecules for preparative, semi-preparative or analytical scale separations." -Analytical and Bioanalytical Chemistry, Oct,24-2014