Liquid Chromatography

Liquid Chromatography

Applications

1st Edition - January 8, 2013

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  • Editors: Salvatore Fanali, Paul Haddad, Colin Poole, David Lloyd
  • eBook ISBN: 9780124158665

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Description

A single source of authoritative information on all aspects of the practice of modern liquid chromatography suitable for advanced students and professionals working in a laboratory or managerial capacity

Key Features

  • Chapters written by authoritative and visionary experts in the field provide an overview and focused treatment of a single topic
  • Each chapter emphasizes the integration of chromatographic methods and sample preparation, automation, and explains how liquid chromatography is used in different industrial sectors
  • Focuses on expanding and illustrating the main features of the fundamental section, while demonstrating where and how the best practices of liquid chromatography are utilized
  • Comprehensive coverage of modern liquid chromatography from theory, to methods, to selected applications
  • Thorough selected references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision making

Readership

Practitioners of distillation and separation science looking for a quick access to the newest knowledge; graduate students searching for special applications; chemists; professional scientists in academia, industry and government laboratories; environmental engineers; mechanical engineers. 70% professionals, 20% students, 10% others

Table of Contents

  • Contributors

    Chapter 1. Affinity Chromatography

    1.1 Introduction

    1.2 Basic Components of Affinity Chromatography

    1.3 Bioaffinity Chromatography

    1.4 Immunoaffinity Chromatography

    1.5 Dye-Ligand and Biomimetic Affinity Chromatography

    1.6 Immobilized Metal-Ion Affinity Chromatography

    1.7 Analytical Affinity Chromatography

    1.8 Miscellaneous Methods and Newer Developments

    Acknowledgment

    References

    Chapter 2. Derivatization in Liquid Chromatography

    2.1 Introduction

    2.2 Reagent Selection

    2.3 Postcolumn Reaction Detectors

    2.4 Conclusions

    References

    Chapter 3. Validation of Liquid Chromatographic Methods

    3.1 Traditional Method Validation

    3.2 Quality by Design and Analytical Methods

    3.3 Conclusion

    References

    Chapter 4. Liquid Chromatographic Separation of Enantiomers

    4.1 Introduction

    4.2 A Short History of Chiral Separations by Liquid Chromatography

    4.3 Materials for the Liquid Chromatographic Separation of Enantiomers

    4.4 Modes of Liquid Chromatographic Separation of Enantiomers

    4.5 Separation of Enantiomers by Supercritical Fluid Chromatography

    4.6 Summary and Future Trends

    References

    Chapter 5. Liquid Interaction Chromatography of Polymers

    5.1 Introduction

    5.2 Theoretical Aspects of Isocratic Liquid Chromatography of Polymers

    5.3 Applications of Liquid Chromatography of Polymers

    5.4 Hyphenated Techniques

    5.5 Summary

    References

    Chapter 6. Amino Acid and Bioamine Separations

    6.1 Introduction

    6.2 Direct Separation of Amino Acids and Amines

    6.3 Indirect Separation of Amino Acids and Amines

    6.4 Enantioselective Liquid Chromatographic Analysis of Amino Acids

    6.5 Conclusions

    References

    Chapter 7. Protein and Peptide Separations

    7.1 Introduction

    7.2 Methods of Protein Liquid Chromatography

    7.3 Conclusions

    Acknowledgments

    References

    Chapter 8. Glycans and Monosaccharides

    8.1 Introduction

    8.2 Types of Glycans

    8.3 Analysis and Characterization of Glycans

    8.4 Monosaccharide Composition Analysis

    8.5 Conclusions

    References

    Chapter 9. Separation of Lipids

    9.1 Introduction and Contents

    9.2 Definitions and Classification

    9.3 Structures and Occurrence

    9.4 Sample Extraction and Handling

    9.5 Lipid Analysis by Liquid Chromatography

    9.6 Conclusions and Future Perspectives

    References

    Chapter 10. Forensic Toxicology

    10.1 General Drug Screening

    10.2 Liquid Chromatography-Mass Spectrometry in Forensic Toxicology

    10.3 Testing for Driving Under the Influence of Drugs Using Oral Fluid

    10.4 Toxicological Analysis of Hair in the Investigation of Drug Facilitated Crimes

    10.5 Targeted Poisons

    10.6 Conclusions

    References

    Chapter 11. Compositional Analysis of Foods

    11.1 Introduction

    11.2 Carbohydrates

    11.3 Vitamins

    11.4 Amino Acids, Peptides, and Proteins

    11.5 Lipids

    11.6 Minor Components of Food

    11.7 Food Additives

    11.8 Conclusions and Future Trends

    Acknowledgment

    References

    Chapter 12. Multiresidue Methods for Pesticides and Related Contaminants in Food

    12.1 Introduction

    12.2 Sample Treatment

    12.3 Matrix Effects in LC–MS Analysis

    12.4 Method Validation

    12.5 Analysis of Samples

    12.6 Individual Methods for Specific Compounds

    12.7 LC–TOF MS in the Field of Pesticide Residue Analysis

    References

    Chapter 13. Environmental Analysis: Persistent Organic Pollutants∗

    13.1 Polycyclic Aromatic Hydrocarbons

    13.2 Chlorinated Aromatic Compounds

    13.3 Pesticides

    13.4 Brominated Flame Retardants

    13.5 Perfluoroalkyl Compounds

    13.6 Reference Materials

    13.7 Concluding Remarks

    13.8 Disclaimer

    References

    Chapter 14. Environmental Analysis: Emerging Pollutants

    14.1 Introduction

    14.2 General Trends

    14.3 Target Analysis of Specific Contaminant Groups Using LC–MS

    14.4 Conclusions

    References

    Chapter 15. Analysis of Natural Toxins

    15.1 Introduction

    15.2 Tetrodotoxin

    15.3 Lipophilic Marine Toxins

    15.4 Saxitoxin and Analogs

    References

    Chapter 16. Liquid Chromatography in the Pharmaceutical Industry

    16.1 The Role of Separation Science in Pharmaceutical Drug Development

    16.2 Increasing Chromatographic Resolution

    16.3 Chromatographic Method Development: RPLC

    Acknowledgments

    References

    Chapter 17. Determination of Veterinary Drug Residues in Foods by Liquid Chromatography–Mass Spectrometry: Basic and Cutting-Edge Applications

    17.1 Introduction

    17.2 Options in Veterinary Residue Analysis using LC–MS

    17.3 Conclusions

    References

    Chapter 18. Analysis of Vitamins by Liquid Chromatography

    18.1 Introduction

    18.2 Liquid Chromatographic Determination of Water-Soluble Vitamins

    18.3 Liquid Chromatographic Determination of Fat-Soluble Vitamins

    18.4 Multivitamin Methods

    References

    Chapter 19. Applications of Liquid Chromatography in the Quality Control of Traditional Chinese Medicines, an Overview

    19.1 Introduction

    19.2 Liquid Chromatographic Analysis of Traditional Chinese Medicines

    19.3 Conclusions

    Acknowledgments

    References

    Chapter 20. Analysis of Neurotransmitters and Their Metabolites by Liquid Chromatography

    20.1 Introduction

    20.2 Biogenic Amines

    20.3 Acetylcholine

    20.4 Amino Acids

    20.5 Purines: Adenosine Triphosphate and Adenosine

    20.6 Endocannabinoids

    20.7 Neuropeptides

    20.8 Multianalyte Monitoring of Neurotransmitters from Diverse Classes

    20.9 Clinical Applications of Microdialysis Sampling and Liquid Chromatographic-Based Analysis

    20.10 Conclusions

    References

    Chapter 21. Clinical Chemistry, Including Therapeutic Drug Monitoring and Biomarkers for Diagnosis

    21.1 Introduction

    21.2 Preanalytical Specimen Handling in Clinical Liquid Chromatography

    21.3 Separation Technologies in Clinical Liquid Chromatography

    21.4 Detection Technologies in Clinical Liquid Chromatography

    21.5 Applications

    21.6 Conclusion

    References

    Chapter 22. Speciation and Element-Specific Detection

    22.1 Introduction

    22.2 Sampling

    22.3 Sample Storage and Processing

    22.4 Speciation Approaches: Direct Methods or Hyphenated Techniques

    22.5 Interfacing: Nebulizers, Use of Internal Standard and Postcolumn Dilution

    22.6 Element-Selective Detection

    22.7 Quantification and Quality Control

    References

    Index

Product details

  • No. of pages: 688
  • Language: English
  • Copyright: © Elsevier 2013
  • Published: January 8, 2013
  • Imprint: Elsevier
  • eBook ISBN: 9780124158665

About the Editors

Salvatore Fanali

Salvatore Fanali
Salvatore Fanali is Director of Research at the Institute of Chemical Methodologies, Italian National Research Council (C.N.R.) in Monterotondo (Rome), Italy, and head of the Capillary Electromigration and Chromatographic Methods Unit at the same Institute. His research activity is mainly focused on separation science including the development of modern miniaturized techniques (electrodriven and liquid chromatography). He also studies hyphenation with mass spectrometry and development of new stationary phases. Separation methods developed are currently applied to food, pharmaceuticals, chiral environment, and biomedical analysis. He is Editor of the Journal of Chromatography A and a member of the advisory editorial board of seven international scientific journals. Fanali is the author of about 300 publications including some book chapters. He received several awards including the “Liberti Medal” in Separation Science from the Italian Chemical Society.

Affiliations and Expertise

Director and Head, Capillary Electromigration and Chromatographic Methods Unit, Institute of Chemical Methodologies, Italian National Research Council (C.N.R.), Monterotondo, Rome, Italy

Paul Haddad

Paul Haddad
Paul Haddad is currently a Distinguished Professor of Chemistry and Australian Research Council Federation Fellow at the University of Tasmania, as well as Director of the Pfizer Analytical Research Centre. He has more than 500 publications in this field and has presented in excess of 450 papers at local and international scientific meetings. He is an editor of Journal of Chromatography A, a contributing editor for Trends in Analytical Chemistry, and was an editor of Analytica Chimica Acta for 6 years. He is currently a member of the editorial boards of 10 other journals of analytical chemistry or separation science. He is the recipient of several national and international awards, including the ACS Award in Chromatography, the Marcel Golay Award, the AJP Martin Gold Medal awarded by the Chromatographic Society, the Royal Society of Chemistry Analytical Separation Methods Award, the RACI HG Smith and Analytical Division medals, and more.

Affiliations and Expertise

Distinguished Professor, School of Chemistry, University of Tasmania, Hobart, Tasmania, Australia

Colin Poole

Colin Poole
Professor Colin Poole is internationally known in the field of thin-layer chromatography and is an editor of the Journal of Chromatography and former editor of the Journal of Planar Chromatography – Modern TLC. He has authored several books on chromatography, recent examples being The Essence of Chromatography published by Elsevier (2003), and Gas Chromatography published by Elsevier (2012). He is the author of approximately 400 research articles, many of which deal with thin-layer chromatography, and is co-chair of the biennial “International Symposium on High-Performance Thin-Layer Chromatography”.

Affiliations and Expertise

Department of Chemistry, Wayne State University, Detroit, MI, USA

David Lloyd

David Lloyd
David Lloyd obtained his PhD from the Department of Chemistry, University of York, UK, working with Prof. David Goodall on the development of laser-based polarimetry as a chiral detection method. He then completed postdoctoral research on bioanalytical capillary electrophoresis (CE) with Prof. Irving Wainer at St. Jude Children’s Research Hospital in Memphis, TN. He has since worked both in academia (McGill University, Departments of Oncology and Experimental Medicine) and the pharmaceutical industry (DuPont Pharmaceuticals and Bristol-Myers Squibb). Whilst at McGill, Dr. Lloyd’s analytical research focused on microscale bioanalysis by CE and on chiral CE. Since moving to the pharmaceutical industry, his responsibilities have been in analytical R&D for projects ranging from the late discovery stage through worldwide registrational filings, with a primary focus on separations science. From 1995 – 2002, Dr. Lloyd was Contributing Editor of TrAC - Trends in Analytical Chemistry; and from 1999 - 2011, he was Editor of the Journal of Chromatography B.

Affiliations and Expertise

Bristol-Myers Squibb, New Brunswick, NJ, USA

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