Lipolytic Enzymes  - 1st Edition - ISBN: 9780121345501, 9780323155168

Lipolytic Enzymes

1st Edition

Authors: Hans Brockerhoff
eBook ISBN: 9780323155168
Imprint: Academic Press
Published Date: 28th January 1974
Page Count: 342
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Description

Lipolytic Enzymes focuses on the biochemistry of lipolytic enzymes, particularly, pancreatic lipase and phospholipase 2 as well as their structure and catalytic mechanism. It explores the kinetics of lipolysis, the digestive lipases of nonmammalian animals, the assay and purification of cholesterol esterases and phospholipases, the method phospholipases use in hydrolyzing phospholipids, and the adaptive mechanism of lipolytic enzymes at the lipid-water interface. Organized into eight chapters, this book begins with an overview of the importance of lipolytic enzymes, including their medical, therapeutic, food, and other industrial applications. It then proceeds with a discussion on the classification of lipolytic enzymes according to the type of bond they hydrolyze and the substrates on which they act. The next chapters look at the substrates and supersubstrates of lipolytic enzymes, along with their maximal velocity and the Michaelis constant. Moreover, the book talks about the detection and assay of lipases, the molecular properties of pancreatic cholesterol esterases, the stimulating effect of bile salts on cholesterol esterases, the hydrolytic cleavage of carboxyl esterases, and the occurrence and distribution of phosphohydrolases. A chapter discussing the two groups of lipolytic enzymes (the first containing enzymes of broad substrate specificity and not requiring cofactors; the second containing metalloenzymes with very narrow substrate requirements) concludes this book. This book is a valuable resource for chemists, biochemists, and those working in the field of nutritional sciences.

Table of Contents

Preface Abbreviations and Definitions I. Introduction Importance of Lipolytic Enzymes II. Nomenclature I. Substrates II. Table of Lipolytic Enzymes III. Kinetics of Lipolysis I. Substrate and Supersubstrate II. Maximal Velocity III. Determination of the Km IV. Meaning of Km V. Inhibition at the Interface VI. pH and Cations at the Interface VII. Monolayers VIII. Water-Soluble Substrates IV. Lipases I. Detection and Assay II. Pancreatic Lipase III. Gastric, Pregastric, and Lingual Lipases IV. Digestive Lipases of Nonmammalian Animals V. Lipoprotein Lipase VI. Tissue Lipases VII. Milk Lipases VIII. Plant Lipases IX. Microbial Lipases X. Monoglyceride Lipases XI. Glycosyldiglyceride Lipases V. Cholesterol Esterase I. Assay II. Purification III. Molecular Properties of Pancreatic Cholesterol Esterases IV. Substrate Specificity V. Inhibitors VI. Requirement for Bile Salts VII. Intracellular Cholesterol Esterases VIII. Conclusion VI. Phospholipases: Carboxyl Esterases I. Phospholipase 2 II. Phospholipase 1 III. Lysophospholipase VII. Phospholipases: Phosphohydrolases I. Phosphatidate Phosphohydrolase II. Phospholipase 3 III. Sphingomyelinase IV. Phospholipase 4; Ceramidase VIII. Synopsis I. The Nonspecific Lipolytic Enzymes II. Metal-Dependent Lipolytic Enzymes III. Nature of Lipolytic Enzymes Bibliography Subject Index

Details

No. of pages:
342
Language:
English
Copyright:
© Academic Press 1974
Published:
Imprint:
Academic Press
eBook ISBN:
9780323155168

About the Author

Hans Brockerhoff

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