Lipolytic Enzymes  - 1st Edition - ISBN: 9780121345501, 9780323155168

Lipolytic Enzymes

1st Edition

Authors: Hans Brockerhoff
eBook ISBN: 9780323155168
Imprint: Academic Press
Published Date: 28th January 1974
Page Count: 342
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Lipolytic Enzymes focuses on the biochemistry of lipolytic enzymes, particularly, pancreatic lipase and phospholipase 2 as well as their structure and catalytic mechanism. It explores the kinetics of lipolysis, the digestive lipases of nonmammalian animals, the assay and purification of cholesterol esterases and phospholipases, the method phospholipases use in hydrolyzing phospholipids, and the adaptive mechanism of lipolytic enzymes at the lipid-water interface. Organized into eight chapters, this book begins with an overview of the importance of lipolytic enzymes, including their medical, therapeutic, food, and other industrial applications. It then proceeds with a discussion on the classification of lipolytic enzymes according to the type of bond they hydrolyze and the substrates on which they act. The next chapters look at the substrates and supersubstrates of lipolytic enzymes, along with their maximal velocity and the Michaelis constant. Moreover, the book talks about the detection and assay of lipases, the molecular properties of pancreatic cholesterol esterases, the stimulating effect of bile salts on cholesterol esterases, the hydrolytic cleavage of carboxyl esterases, and the occurrence and distribution of phosphohydrolases. A chapter discussing the two groups of lipolytic enzymes (the first containing enzymes of broad substrate specificity and not requiring cofactors; the second containing metalloenzymes with very narrow substrate requirements) concludes this book. This book is a valuable resource for chemists, biochemists, and those working in the field of nutritional sciences.

Table of Contents


Abbreviations and Definitions

I. Introduction

Importance of Lipolytic Enzymes

II. Nomenclature

I. Substrates

II. Table of Lipolytic Enzymes

III. Kinetics of Lipolysis

I. Substrate and Supersubstrate

II. Maximal Velocity

III. Determination of the Km

IV. Meaning of Km

V. Inhibition at the Interface

VI. pH and Cations at the Interface

VII. Monolayers

VIII. Water-Soluble Substrates

IV. Lipases

I. Detection and Assay

II. Pancreatic Lipase

III. Gastric, Pregastric, and Lingual Lipases

IV. Digestive Lipases of Nonmammalian Animals

V. Lipoprotein Lipase

VI. Tissue Lipases

VII. Milk Lipases

VIII. Plant Lipases

IX. Microbial Lipases

X. Monoglyceride Lipases

XI. Glycosyldiglyceride Lipases

V. Cholesterol Esterase

I. Assay

II. Purification

III. Molecular Properties of Pancreatic Cholesterol Esterases

IV. Substrate Specificity

V. Inhibitors

VI. Requirement for Bile Salts

VII. Intracellular Cholesterol Esterases

VIII. Conclusion

VI. Phospholipases: Carboxyl Esterases

I. Phospholipase 2

II. Phospholipase 1

III. Lysophospholipase

VII. Phospholipases: Phosphohydrolases

I. Phosphatidate Phosphohydrolase

II. Phospholipase 3

III. Sphingomyelinase

IV. Phospholipase 4; Ceramidase

VIII. Synopsis

I. The Nonspecific Lipolytic Enzymes

II. Metal-Dependent Lipolytic Enzymes

III. Nature of Lipolytic Enzymes


Subject Index


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© Academic Press 1974
Academic Press
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About the Author

Hans Brockerhoff

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