Description

Lipids are a broad group of naturally occurring molecules which includes fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides, phospholipids, and others. The main biological functions of lipids include energy storage, as structural components of cell membranes, and as important signaling molecules.
This volume of Methods in Cell Biology covers such areas as Membrane structure and dynamics, Imaging, and Lipid Protein Interactions. It will be an essential tool for researchers and students alike.

Key Features

  • Covers such areas as membrane structure and dynamics, imaging, and lipid protein interactions
  • An essential tool for researchers and students alike
  • International authors
  • Renowned editors

Readership

Researchers and students in cell, molecular and developmental biology

Table of Contents

Contributors

Preface

Part I: Membrane Dynamics and Reconstitution Assays

Chapter 1: Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding

I. Introduction

II. Preparation of Membrane Sheets

III. Preparation of Brain Extract

IV. Cell-Free Reaction

V. Conclusions

Chapter 2: Studying Lipids Involved in the Endosomal Pathway

I. Introduction

II. Methods

III. Conclusion

Appendix A. Supplementary Movies

Chapter 3: Studying In Vitro Membrane Curvature Recognition by Proteins and its Role in Vesicular Trafficking

I. Introduction

II. Preparation of proteins and liposomes

III. Binding Assays for Testing Curvature Recognition by a Protein

IV. Distribution of a Curvature-Sensing Protein on Tube Networks Pulled by Kinesin Motors

V. Distribution of a Curvature-Sensing Protein on a Tube Elongated by Optical Tweezers

VI. Assays to Measure the Curvature-Dependant Activity of ArfGAPl and GMAP-210

VII. Summary and Conclusion

Chapter 4: Reconstituting Multivesicular Body Biogenesis with Purified Components

I. Introduction

II. Rationale

III. Methods

IV. Materials

V. Discussion

VI. Summary and Outlook

Chapter 5: Approaches to the Study of Atg8-Mediated Membrane Dynamics In Vitro

I. Introduction

II. Using Liposomes as In Vitro Mimics of Autophagosome Membrane

III. Proteins

IV. The Lipidation Reaction

V. Alternative Lipidation Approach for PE and Enzyme Independence

VI. Membrane Tethering

VII. Conclusion

Chapter 6: Reconstitution Assay System for Ceramide Transport With Semi-Intact Cells

I. Introduction

II. Materials

III. Methods

IV. Notes

Chapter 7: Visualizing Mitocho

Details

No. of pages:
528
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
Print ISBN:
9780123864871
Electronic ISBN:
9780123864888