Lipids are a broad group of naturally occurring molecules which includes fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides, phospholipids, and others. The main biological functions of lipids include energy storage, as structural components of cell membranes, and as important signaling molecules.
This volume of Methods in Cell Biology covers such areas as Membrane structure and dynamics, Imaging, and Lipid Protein Interactions. It will be an essential tool for researchers and students alike.

Key Features

  • Covers such areas as membrane structure and dynamics, imaging, and lipid protein interactions
  • An essential tool for researchers and students alike
  • International authors
  • Renowned editors


Researchers and students in cell, molecular and developmental biology

Table of Contents

Membrane Dynamics and Reconstitution Assays:

  1. Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding
  2. Min Wu and Pietro De Camilli

  3. Studying lipids involved in the endosomal pathway
  4. Christin Bissig, Shem Johnson, and Jean Gruenberg

  5. Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking
  6. Jean-Baptise Manneville, Cécile Leduc, Benoit Sorre, and Guillaume Drin

  7. Reconstituting Multivesicular Body Biogenesis with purified components
  8. Thomas Wollert

  9. Approaches to the study of Atg8-mediated membrane dynamics in vitro
  10. Anjali Jotwani, Diana N. Richerson, Isabelle Motta, Omar Julca-Zevallos, and Thomas J. Melia

  11. Reconstitution assay system for ceramide transport with semi-intact cells
  12. Keigo Kumagai, Masahiro Nishijim, Kentaro Hanada

  13. Visualizing mitochondrial lipids and fusion events in mammalian cells
  14. Huiyan Huang and Michael A. Frohman

    Lipid Metabolism and Signaling:

  15. Targeted and non-targeted analysis of membrane lipids using mass spectrometry
  16. Xue Li Guan and Markus R. Wenk

  17. Modulation of host phosphoinositide metabolism during Salmonella invasion by the type III secreted effector SopB
  18. Bernhard Roppenser, Sergio Grinstein and John H. Brumell

  19. Acute Manipulation of Phosphoinositide Levels in Cells
  20. Belle Chang-Ileto, Samuel G. Frere, and Gilbert Di Paolo

  21. Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins
  22. Marnix Wieffer, Volker Haucke and Michael Krauß

  23. Phosphoinositides at the neuromuscular


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© 2012
Academic Press
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