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Description
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry; Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids; Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins; Biochemical Analysis of Phospholipase D.; Measurement of Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor; Quantitative measurement of PtdIns(3,4,5)P3; Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes; Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins; Biochemical Analysis of Inositol Phosphate Kinases; Analysis of the phosphoinositides and their aqueous metabolites; Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism; Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo; A rapid and sensitive method to measure secretion of sphingosine-1-phosphate; Ceramide Kinase and Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases from Saccharomyces cerevisiae.
Readership
Graduate students, researchers in academics and industry studying biochemistry.
Table of Contents
Chapter 1
Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry
REI MORIKAWA, MASAFUMI TSUJIMOTO, HIROYUKI ARAI and JUNKEN AOKI
Chapter 2
Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids
Debasis Manna, Wonhwa Cho
Chapter 3
Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins
David M. Lehmann, Chujun Yuan and Alan V. Smrcka
Chapter 4
Biochemical Analysis of Phospholipase D.
H. Alex Brown, Lee G. Henage, Anita M. Preininger, Yun Xiang, John H. Exton
Chapter 5
Measurement of Autotaxin/Lysophospholipase D Activity
Andrew J. Morris and Susan S. Smyth
Chapter 6
Platelet-Activating Factor
John S. Owen, Michael J. Thomas, and Robert L. Wykle
Chapter 7
Quantitative measurement of PtdIns(3,4,5)P3
H. Guillou, L. Stephens, P.T. Hawkins
Chapter 8
Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes
Amber C. Donahue, Michael G. Kharas, and David A. Fruman
Chapter 9
Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins
Yasunori Kanaho, Kazuhisa Nakayama, Michael A. Frohman, and Takeaki Yokozeki
Chapter 10
Biochemical Analysis of Inositol Phosphate Kinases
James C. Otto, Sashidhar Mulugu, Peter C. Fridy, Shean-Tai Chiou, Blaine N. Armbruster, Anthony A. Ribeiro and John D. York
Chapter 11
Analysis of the phosphoinositides and their aqueous metabolites
Christoph
Details
- No. of pages:
- 392
- Language:
- English
- Copyright:
- © 2007
- Published:
- 12th November 2007
- Imprint:
- Academic Press
- eBook ISBN:
- 9780080554099
- Print ISBN:
- 9780123739650