Lipidomics and Bioactive Lipids: Lipids and Cell Signaling, Volume 434

1st Edition

Serial Volume Editors: H. Alex Brown
Hardcover ISBN: 9780123739650
eBook ISBN: 9780080554099
Imprint: Academic Press
Published Date: 12th November 2007
Page Count: 392
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Table of Contents

Chapter 1 Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry REI MORIKAWA, MASAFUMI TSUJIMOTO, HIROYUKI ARAI and JUNKEN AOKI Chapter 2 Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids Debasis Manna, Wonhwa Cho Chapter 3 Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins David M. Lehmann, Chujun Yuan and Alan V. Smrcka Chapter 4 Biochemical Analysis of Phospholipase D. H. Alex Brown, Lee G. Henage, Anita M. Preininger, Yun Xiang, John H. Exton Chapter 5 Measurement of Autotaxin/Lysophospholipase D Activity Andrew J. Morris and Susan S. Smyth Chapter 6 Platelet-Activating Factor John S. Owen, Michael J. Thomas, and Robert L. Wykle Chapter 7 Quantitative measurement of PtdIns(3,4,5)P3 H. Guillou, L. Stephens, P.T. Hawkins Chapter 8 Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes Amber C. Donahue, Michael G. Kharas, and David A. Fruman Chapter 9 Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins Yasunori Kanaho, Kazuhisa Nakayama, Michael A. Frohman, and Takeaki Yokozeki Chapter 10 Biochemical Analysis of Inositol Phosphate Kinases James C. Otto, Sashidhar Mulugu, Peter C. Fridy, Shean-Tai Chiou, Blaine N. Armbruster, Anthony A. Ribeiro and John D. York Chapter 11 Analysis of the phosphoinositides and their aqueous metabolites Christoph

Description

This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry; Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids; Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins; Biochemical Analysis of Phospholipase D.; Measurement of Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor; Quantitative measurement of PtdIns(3,4,5)P3; Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes; Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins; Biochemical Analysis of Inositol Phosphate Kinases; Analysis of the phosphoinositides and their aqueous metabolites; Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism; Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo; A rapid and sensitive method to measure secretion of sphingosine-1-phosphate; Ceramide Kinase and Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases from Saccharomyces cerevisiae.

Readership

Graduate students, researchers in academics and industry studying biochemistry.


Details

No. of pages:
392
Language:
English
Copyright:
© Academic Press 2007
Published:
Imprint:
Academic Press
eBook ISBN:
9780080554099
Hardcover ISBN:
9780123739650

About the Serial Volume Editors

H. Alex Brown Serial Volume Editor