Lipidomics and Bioactive Lipids: Lipids and Cell Signaling

Lipidomics and Bioactive Lipids: Lipids and Cell Signaling

1st Edition - November 12, 2007
This is the Latest Edition
  • Editor: H. Alex Brown
  • eBook ISBN: 9780080554099
  • Hardcover ISBN: 9780123739650

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Description

This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry; Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids; Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins; Biochemical Analysis of Phospholipase D.; Measurement of Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor; Quantitative measurement of PtdIns(3,4,5)P3; Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes; Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins; Biochemical Analysis of Inositol Phosphate Kinases; Analysis of the phosphoinositides and their aqueous metabolites; Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism; Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo; A rapid and sensitive method to measure secretion of sphingosine-1-phosphate; Ceramide Kinase and Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases from Saccharomyces cerevisiae.

Readership

Graduate students, researchers in academics and industry studying biochemistry.

Table of Contents

  • Chapter 1
    Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry
    REI MORIKAWA, MASAFUMI TSUJIMOTO, HIROYUKI ARAI and JUNKEN AOKI

    Chapter 2
    Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids
    Debasis Manna, Wonhwa Cho

    Chapter 3
    Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins
    David M. Lehmann, Chujun Yuan and Alan V. Smrcka

    Chapter 4
    Biochemical Analysis of Phospholipase D.
    H. Alex Brown, Lee G. Henage, Anita M. Preininger, Yun Xiang, John H. Exton

    Chapter 5
    Measurement of Autotaxin/Lysophospholipase D Activity
    Andrew J. Morris and Susan S. Smyth

    Chapter 6
    Platelet-Activating Factor
    John S. Owen, Michael J. Thomas, and Robert L. Wykle

    Chapter 7
    Quantitative measurement of PtdIns(3,4,5)P3
    H. Guillou, L. Stephens, P.T. Hawkins

    Chapter 8
    Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes
    Amber C. Donahue, Michael G. Kharas, and David A. Fruman

    Chapter 9
    Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins
    Yasunori Kanaho, Kazuhisa Nakayama, Michael A. Frohman, and Takeaki Yokozeki

    Chapter 10
    Biochemical Analysis of Inositol Phosphate Kinases
    James C. Otto, Sashidhar Mulugu, Peter C. Fridy, Shean-Tai Chiou, Blaine N. Armbruster, Anthony A. Ribeiro and John D. York

    Chapter 11
    Analysis of the phosphoinositides and their aqueous metabolites
    Christopher P. Berrie, Cristiano Iurisci, Enza Piccolo, Renzo Bagnati and Daniela Corda

    Chapter 12
    Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism.
    Stefka Spassieva, Jacek Bielawski, Viviana Anelli, and Lina M. Obeid

    Chapter 13
    Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo
    Michael Maceyka, Sheldon Milstien, Sarah Spiegel

    Chapter 14
    A rapid and sensitive method to measure secretion of sphingosine-1-phosphate
    Poulami Mitra, Shawn G. Payne, Sheldon Milstien, Sarah Spiegel

    Chapter 15
    Ceramide Kinase and Ceramide-1-Phosphate
    Dayanjan S. Wijesinghe, Nadia F. Lamour, Antonio Gomez-Munoz, and Charles E. Chalfant

    Chapter 16
    Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells
    Richard M. Epand and Matthew K. Topham

    Chapter 17
    Lipid Phosphate Phosphatases from Saccharomyces cerevisiae
    George M. Carman and Wen-I Wu

Product details

  • No. of pages: 392
  • Language: English
  • Copyright: © Academic Press 2007
  • Published: November 12, 2007
  • Imprint: Academic Press
  • eBook ISBN: 9780080554099
  • Hardcover ISBN: 9780123739650

About the Serial Volume Editor

H. Alex Brown