General Description of the Volume: This volume presents the best techniques and strategies for the study of lipid modified proteins, with particular emphasis on methods which evaluate the functional significance or biological impact of lipid modification. Included are the biological methods for the study of function (yeast genetics; cloning strategies; mutational analysis; expression systems), biochemical methods for the study and purification of enzymes or modified proteins (in vitro assays using peptide, native, or recombinant protein substrates; coupled in vitro cDNA transcription, translation/modification; bacolovirus expression; lipid analogs/inhibitors); physical methods for the identification of lipid groups (cleavage techniques; modification techniques; simple separations: TLC, GC, HPLC, ES/MS, tandem MS).
General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Biochemists, biophysicists, microbiologists, analytical chemists, virologists, molecular biologists, and cell biologists.
C.A. Omer, R.E. Diehl, and A.M. Kral, Bacterial Expression and Purification of Human Protein Prenyltransferases Using Epitope-Tagged, Translationally Coupled Systems.
J.F. Moomaw, F.L. Zhang, and P.J. Casey, Isolation of Protein Prenyltransferases from Bovine Brain and Baculovirus Expression System.
Y. Reiss, Substrate Interactions of Protein Prenyltransferases.
P.B. Cassidy, J.M. Dolence, and C.D. Poulter, Continuous Fluorescence Assay for Protein Prenyltransferases.
H. Mitsuzawa and F. Tamanoi, In Vivo Assays for Farnesyltransferase Inhibitors with Saccharomyces cerevisiae.
B.E. Caplin and M.S. Marshall, Mutagenesis and Biochemical Analysis of Recombinant Yeast Prenyltransferases.
D.A. Mitchell and R.J. Deschenes, Characterization of Protein Prenylation in Saccharomyces cerevisiae.
A.L. Wilson and W.A. Maltese, Coupled Translation/Prenylation of Rab Proteins in Vitro. Y. Fukada, Prenylation and Carboxylmethylation of G-Protein gamma Subunit.
A.D. Cox, Mutation and Analysis of Prenylation Signal Sequences.
Y. Takai, K. Kaibuchi, A. Kikuci, and T. Sasaki, Effects of Prenyl Modifications on Interactions of Small G Proteins with Regulators.
M.Dalton and M. Sinensky, Expression Systems for Nuclear Lamin Proteins: Farnesylation in Assembly of Nuclear Lamina.
J. Inglese, Prenylation-Dependent Targeting of G-Protein-Coupled Receptor Kinases.
J.A. Thissen, M.G. Barrett, and P.J. Casey, Prenylated Peptides in Identification of Specific Binding Proteins.
R.M. Epand, F. Naider, and J.M. Becker, Lipid-Mediated a-Factor Interactions with Artificial Membranes.
- No. of pages:
- © Academic Press 1995
- 16th May 1995
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
Duke University Medical Center, Durham, North Carolina, U.S.A.
Iowa State University, Ames, U.S.A.
@from:Praise for the Volume @qu:"...timely and useful... a very well-compiled volume... a substantive stimulus to those in the field, but it is also a very readable means for others to acquire a general introduction." @source:--James F. Riordan, Harvard Medical School, in ANALYTICAL BIOCHEMISTRY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY