Lipid Droplets

Lipid Droplets

1st Edition - October 4, 2013

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  • Editors: H. Robert Yang, Peng Li
  • Hardcover ISBN: 9780124080515
  • eBook ISBN: 9780124095212

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Description

This new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging and in vitro analyses of LD biogenesis and growth. Chapters are written by experts in the field. With cutting-edge material, this comprehensive collection is intended to guide researchers of LDs for years to come.

Key Features

  • Covers sections on analyses of lipid droplets (LDs) in model systems, cell/tissue-specific analyses of LDs and imaging, and in vitro analyses of LD biogenesis and growth
  • Chapters are written by experts in the field
  • Cutting-edge material

Readership

Researchers and students in cell, molecular and developmental biology

Table of Contents

  • Series Page

    Contributors

    Preface

    Chapter 1. Proteomic Studies of Isolated Lipid Droplets from Bacteria, C. elegans, and Mammals

    Abstract

    Introduction

    Acknowledgment

    References

    Chapter 2. Analysis of Yeast Lipid Droplet Proteome and Lipidome

    Abstract

    Introduction

    2.1 Materials

    2.2 Methods

    2.3 Results and Discussion

    2.4 Notes

    Acknowledgments

    References

    Chapter 3. Visualization of Lipid Droplets in C. elegans by Light and Electron Microscopy

    Abstract

    Introduction

    Summary

    Acknowledgments

    References

    Chapter 4. Analysis of Lipid Droplet Dynamics and Functions in Drosophila melanogaster

    Abstract

    4.1 Genome-Wide RNA Interference Screens in Drosophila Cells to Identify Regulators for LD Biology

    4.2 LD Dynamics in Different Drosophila Developmental Stages

    4.3 LD Functions in Drosophila Physiology

    Acknowledgments

    References

    Chapter 5. Analysis of Oil Droplets in Microalgae

    Abstract

    Introduction

    5.1 Nile Red as a Probe for Oil Droplets

    5.2 Confocal and Transmission Electron Microscopic Observation

    5.3 Isolation and Purification of Oil Droplets

    Summary

    Acknowledgments

    References

    Chapter 6. Studying Lipolysis in Adipocytes by Combining siRNA Knockdown and Adenovirus-Mediated Overexpression Approaches

    Abstract

    Introduction and Rationale

    6.1 Materials and Reagents

    6.2 Methods

    Concluding Remarks

    Acknowledgment

    References

    Chapter 7. Analysis of Lipid Droplets in Hepatocytes

    Abstract

    Introduction and Rationale

    7.1 Materials

    7.2 Methods

    7.3 Discussion

    Conclusion

    Acknowledgments

    References

    Chapter 8. Analysis of Lipid Droplets in Cardiac Muscle

    Abstract

    Introduction and Rationale

    8.1 Isolation of Cardiac LD Proteins and Preparation of Cardiac LDs for Analysis by Western Blot

    8.2 Analysis of Cardiac LDs by 2D Analysis of LDs by Conventional TEM

    8.3 Progress in 3D EM Technology May Provide New Tools to Study Cardiac LDs and Mitochondria Interactions

    Conclusions

    Acknowledgments

    References

    Chapter 9. Imaging Cytoplasmic Lipid Droplets in Enterocytes and Assessing Dietary Fat Absorption

    Abstract

    Introduction and Rationale

    9.1 Materials and Methods

    9.2 Discussion

    Acknowledgment

    References

    Chapter 10. Lipid Droplets and Viral Infections

    Abstract

    Introduction

    10.1 Materials

    10.2 Methods

    10.3 Discussion

    Acknowledgments

    References

    Chapter 11. Purification of Integral Membrane Proteins and Lipid-Binding Assays

    Abstract

    Introduction and Rationale

    11.1 Materials

    11.2 Methods

    Conclusion and Applications

    Acknowledgment

    References

    Further Reading

    Chapter 12. Imaging of Neutral Lipids and Neutral Lipid Associated Proteins

    Abstract

    Introduction and Rationale

    12.1 Materials

    12.2 Methods

    12.3 Discussion

    Summary

    Acknowledgments

    References

    Chapter 13. Imaging Lipid Droplets by Electron Microscopy

    Abstract

    Introduction

    13.1 Probing the LD Core

    13.2 Observation of the LD Surface

    13.3 Freeze-Substitution

    13.4 Immunoelectron Microscopy of LD-Associated Proteins

    13.5 Freeze-Fracture Electron Microscopy

    13.6 Future directions

    Acknowledgments

    References

    Chapter 14. Imaging Lipid Droplet Fusion and Growth

    Abstract

    Introduction

    Conclusions

    Acknowledgments

    References

    Index

    Volumes in Series

Product details

  • No. of pages: 270
  • Language: English
  • Copyright: © Academic Press 2013
  • Published: October 4, 2013
  • Imprint: Academic Press
  • Hardcover ISBN: 9780124080515
  • eBook ISBN: 9780124095212

About the Editors

H. Robert Yang

Affiliations and Expertise

Faculty of Science, University of South Wales, Australia

Peng Li

Affiliations and Expertise

School of Life Sciences, Tsinghua University, China

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