Description

This new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging and in vitro analyses of LD biogenesis and growth. Chapters are written by experts in the field.

With cutting-edge material, this comprehensive collection is intended to guide researchers of LDs for years to come.

Key Features

  • Covers sections on analyses of lipid droplets (LDs) in model systems, cell/tissue-specific analyses of LDs and imaging, and in vitro analyses of LD biogenesis and growth
  • Chapters are written by experts in the field

  • Cutting-edge material

Readership

Researchers and students in cell, molecular and developmental biology

Table of Contents

Series Page

Contributors

Preface

Chapter 1. Proteomic Studies of Isolated Lipid Droplets from Bacteria, C. elegans, and Mammals

Abstract

Introduction

Acknowledgment

References

Chapter 2. Analysis of Yeast Lipid Droplet Proteome and Lipidome

Abstract

Introduction

2.1 Materials

2.2 Methods

2.3 Results and Discussion

2.4 Notes

Acknowledgments

References

Chapter 3. Visualization of Lipid Droplets in C. elegans by Light and Electron Microscopy

Abstract

Introduction

Summary

Acknowledgments

References

Chapter 4. Analysis of Lipid Droplet Dynamics and Functions in Drosophila melanogaster

Abstract

4.1 Genome-Wide RNA Interference Screens in Drosophila Cells to Identify Regulators for LD Biology

4.2 LD Dynamics in Different Drosophila Developmental Stages

4.3 LD Functions in Drosophila Physiology

Acknowledgments

References

Chapter 5. Analysis of Oil Droplets in Microalgae

Abstract

Introduction

5.1 Nile Red as a Probe for Oil Droplets

5.2 Confocal and Transmission Electron Microscopic Observation

5.3 Isolation and Purification of Oil Droplets

Summary

Acknowledgments

References

Chapter 6. Studying Lipolysis in Adipocytes by Combining siRNA Knockdown and Adenovirus-Mediated Overexpression Approaches

Abstract

Introduction and Rationale

6.1 Materials and Reagents

6.2 Methods

Concluding Remarks

Acknowledgment

References

Chapter 7. Analysis of Lipid Droplets in Hepatocytes

Abstract

Introduction and Rationale

7.1 Materials

7.2 Methods

7.3 Discussion

Conclusion

Acknowledgments

References

C

Details

No. of pages:
270
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Print ISBN:
9780124080515
Electronic ISBN:
9780124095212