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Lipases, Part A: Biotechnology - 1st Edition - ISBN: 9780121821852, 9780080883960

Lipases, Part A: Biotechnology, Volume 284

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editors: Byron Rubin Edward Dennis
eBook ISBN: 9780080883960
Imprint: Academic Press
Published Date: 2nd September 1997
Page Count: 408


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Table of Contents

Primary and Tertiary Structure: M. Cygler and J.D. Schrag, Structure as a Basis for Understanding the Interfacial Properties of Lipases. F. Drablos and S.B. Petersen, Identification of Conserved Residues in a Family of Esterase and Lipase Sequences. S.B. Petersen, F. Drablos, M.T. Neves, and E.I. Petersen, Identification of Important Motifs in Protein Sequences: The Program Multim and Its Applications to Lipase Related Sequences. J.D. Schrag and M. Cygler, Lipases and the (/( Hydrolase Fold. C. Cambillau, Y. Bourne, M.P. Egloff, C. Martinez, and H. van Tilbeurgh, Pancreatic Lipases and Their Complexes with Colipases and Inhibitors: Crystallization and Crystal Packing. M.R. Egmond and C.J.van Bemmel, Impact of Structural Information on Understanding Lipolytic Function. M.T. Neves, P. Martel, E.I. Petersen, F. Drablos, and S.B. Petersen, Surface and Electrostatics of Cutinases. Isolation, Cloning, Expression, and Engineering: M.E. Lowe, Site-Specific Mutagenesis of Human Pancreatic Lipase. H. Wong, R.C. Davis, J.S. Hill, D. Yang, and M. Schotz, Lipase Engineering: A Window into Structure-Function Relationships. L. Blackberg, R.-D. Duan, and B. Sternby,Purification of Carboxyl Ester Lipase (Bile Salt-Stimulated Lipase) from Human Milk and Pancreas. C. Schmidt-Dannert, M.L. R a, and R.D. Schmid, Two Novel Lipases from the Thermophile Bacillus thermocatenulatus: Screening, Purification, Cloning, Overexpression, and Properties. P. Adlercreutz, T. Gitlesen, I. Ncube, and J.S. Read, Vernonia Lipase: A Plant Lipase with Strong Fatty Acid Selectivity. J.S. Hill, R.C. Davis, D. Yang, M.C. Schotz, and H. Wong, Hepatic Lipase: High Level Expression and Subunit Structure Determination. L. Alberghina and M. Lotti, Cloning, Sequencing, and Expression of Candida rugosa Lipases. L. Dupuis, S. Canaan, M. Riviore, and C. Wicker-Planquart, Influence of Various Signal Peptides onSecretion of Mammalian Acidic Lipases in the Baculovirus/Insect Cell System. C. Holm, J.A. Contreras, R. Verger, and M.C. Schotz, Large-Scale Purification and Kinetic Properties of Recombinant Hormone-Sensitive Lipase from Baculovirus/Insect Cell Systems. M.E. Lowe, New Pancreatic Lipases: Gene Expression, Protein Secretion, and the Newborn. M. Boston, C. Requadt, S. Danko, A. Jarnagin, E. Ashizawa, S. Wu, A.J. Poulose, and R. Bott, Structure and Function of Engineered Pseudomonas mendocina Lipase. A. Svendsen, I.G. Clausen, S.A. Patkar, K. Borch, and M. Thellersen, Protein Engineering of Microbial Lipases with Industrial Interest. E. Mas, M.-O. Sadoulet, A. El Battari, and D. Lombardo, Glycosylation of Bile Salt-Dependent Lipase (Cholesterol Esterase). L. Haalck, F. Paltauf, J. Pleiss, R.D. Schmid, F. Spener, and P. Stadler, Steroselectivity of Lipase from Rhizopus oryzae toward Triacylglycerols and Analogs: Computer Aided Modeling and Experimental Validation. Author Index. Subject Index.


The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.


Biochemists; researchers in the pharmaceutical industry; molecular biologists; detergent developers; biophysicists; and organic chemists.


No. of pages:
© Academic Press 1997
2nd September 1997
Academic Press
eBook ISBN:


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About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

Byron Rubin

Affiliations and Expertise

Lipomed, San Diego, California, U.S.A.

Edward Dennis

Edward A. Dennis: University of California, San Diego Distinguished Professor of Chemistry and Biochemistry and former Department Chair and Professor of Pharmacology in the School of Medicine, Dennis is currently Editor-in-Chief of the Journal of Lipid Research and Director of the LIPID MAPS initiative. He also served as Chair of the Board of Directors of the Keystone Symposia on Molecular and Cellular Biology and current as an Emeritus member of the Board of Directors. His current research focus is on phospholipases, cell signaling and lipidomics on which he has published over 300 papers, most related to signal transduction by lipid messengers

Affiliations and Expertise

University of California at San Diego, La Jolla, CA, U.S.A.