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Linkers in Biomacromolecules - 1st Edition - ISBN: 9780128208182, 9780128208236

Linkers in Biomacromolecules, Volume 647

1st Edition

Serial Volume Editor: Maarten Merkx
eBook ISBN: 9780128208236
Hardcover ISBN: 9780128208182
Imprint: Academic Press
Published Date: 20th January 2021
Page Count: 318
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Table of Contents

Preface
Maarten Merkx

1.Understanding and applications of Ser/Gly linkers in protein engineering
Eric Ceballos-Alcantarilla and Maarten Merkx

2.Design and construction of chimeric linker linkers with controllable flexibilities for precision protein engineering
Ziliang Huang, Chong Zhang and Xin-Hui Xing

3.Engineering crowding sensitivity into protein linkers
Theodoros Pittas, Weiyan Zuo and Arnold J. Boersma

4.protaTETHER: A method for the incorporation of linkers in biomacromolecules
Anna I. Wurz, Collin T. O'Bryant and Robert M. Hughes

5.Optimizing the linker length for fusing an alcohol dehydrogenase with a cyclohexanone monooxygenase
Alejandro Gran-Scheuch, Friso Aalbers, Yannick Woudstra, Loreto Parra and Marco W. Fraaije

6.Predicting the effect of disordered linkers on effective concentrations and avidity with the “Ceff calculator” app
Magnus Kjaergaard, Juliana Glavina and Lucia Beatriz Chemes

7.ER/K-link—Leveraging a native protein linker to probe dynamic cellular interactions
Tejas M. Gupte, Michael Ritt and Sivaraj Sivaramakrishnan

8.Design of helical linkers for fusion proteins and protein-based nanostructures
Ryoichi Arai

9.Synthetic protein switches: Combinatorial linker engineering with iFLinkC
Alexander Gräwe, Jan Ranglack, Anastasia Weyrich and Viktor Stein

10.Enzyme-DNA chimeras: Construction, allostery, applications
Chiao-Yu Tseng, Yong Wang and Giovanni Zocchi

11.The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistry
Ri Wang and Fei Sun


Description

Linkers in Biomacromolecules, Volume 647 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this updated release include chapters devoted to ser-gly linkers + models, sergGly- EAAAK linkers, Effect of crowding on linker behavior, Protathether. Experimental approach to quickly test different linkers in fusion proteins, Linkers in enzyme fusions/cascade catalysis, Linker dependence of avidity in multivalent interactions between disordered proteins, ER/K α-helix linker, The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein design, Linker design in protein-based nanostructures, and much more. Linkers in Biomacromolecules, Volume 647 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this updated release include chapters devoted to ser-gly linkers + models, sergGly- EAAAK linkers, Effect of crowding on linker behavior, Protathether. Experimental approach to quickly test different linkers in fusion proteins, Linkers in enzyme fusions/cascade catalysis, Linker dependence of avidity in multivalent interactions between disordered proteins, ER/K α-helix linker, The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein design, Linker design in protein-based nanostructures, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Details

No. of pages:
318
Language:
English
Copyright:
© Academic Press 2021
Published:
20th January 2021
Imprint:
Academic Press
eBook ISBN:
9780128208236
Hardcover ISBN:
9780128208182

Ratings and Reviews


About the Serial Volume Editor

Maarten Merkx

Maarten Merkx is at Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands

Affiliations and Expertise

Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands