Light and Video Microscopy

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation.

• Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope

• Clearly develops all ideas from historical and logical foundations

• Laboratory exercises included to assist the reader with practical applications

• Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope

cell biologists, neurobiologists, reproductive biologists, plant biologists, microbiologists, fungal biologists, veterinarians, forensic scientists, food scientists, horticulturalists, biochemists, physicists, engineers, and anyone using a light or video microscope

(tentative)

Chapter 1: The Relation Between the Object and the Image Chapter 2: Geometrical Optics Chapter 3: Physical Optics Chapter 4: Bright Field Microscopy Chapter 5: Phase Contrast and Dark Field Microscopy, Rheinberg and Oblique Illumination Chapter 6: Photomicrography Chapter 7: Fluorescence Microscopy Chapter 8: Polarization Microscopy Chapter 9: Interference Microscopy Chapter 10: Differential Interference Microscopy Chapter 11: Modulation Contrast Microscopy Chapter 12: Video and Digital Microscopy Chapter 13: Image Processing and Analysis Chapter 14: Various Kinds of Microscopes and Accessories