Light and Video Microscopy - 1st Edition - ISBN: 9780123742346, 9780080921280

Light and Video Microscopy

1st Edition

Authors: Randy Wayne Randy Wayne
Hardcover ISBN: 9780123742346
eBook ISBN: 9780080921280
Imprint: Academic Press
Published Date: 8th August 2008
Page Count: 312
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The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation.

Key Features

  • Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope

  • Clearly develops all ideas from historical and logical foundations

  • Laboratory exercises included to assist the reader with practical applications

  • Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope


cell biologists, neurobiologists, reproductive biologists, plant biologists, microbiologists, fungal biologists, veterinarians, forensic scientists, food scientists, horticulturalists, biochemists, physicists, engineers, and anyone using a light or video microscope

Table of Contents


Chapter 1: The Relation Between the Object and the Image Chapter 2: Geometrical Optics Chapter 3: Physical Optics Chapter 4: Bright Field Microscopy Chapter 5: Phase Contrast and Dark Field Microscopy, Rheinberg and Oblique Illumination Chapter 6: Photomicrography Chapter 7: Fluorescence Microscopy Chapter 8: Polarization Microscopy Chapter 9: Interference Microscopy Chapter 10: Differential Interference Microscopy Chapter 11: Modulation Contrast Microscopy Chapter 12: Video and Digital Microscopy Chapter 13: Image Processing and Analysis Chapter 14: Various Kinds of Microscopes and Accessories


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© Academic Press 2009
Academic Press
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About the Author

Randy Wayne

Affiliations and Expertise

Department of Plant Biology Cornell University, Ithaca, NY, USA

Randy Wayne

Affiliations and Expertise

Department of Plant Biology Cornell University, Ithaca, NY, USA