Laser Tweezers in Cell Biology

Laser Tweezers in Cell Biology

1st Edition - October 17, 1997

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  • Editor: Michael Sheetz
  • eBook ISBN: 9780080859538

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Volume 55 in Methods in Cell Biology is a concise laboratory book that emphasizes the methods and technologies needed to use single polarized laser light source that functions simultaneously as an optical trap and a dual-beam interferometer.

Key Features

* Provides a practical laboratory guide for methods and technologies used with laser tweezers
* Includes comprehensive and easy-to-follow protocols


Graduate students, technicians, postdoctoral, and experienced researchers. Cell, developmental, and molecular biologists, geneticists, human genome researchers, and others studying nuclear structure and organization.

Table of Contents

  • Preface.
    A. Ashkin, Forces of a Single-Beam Gradient Laser Trap on a Dielectric Sphere in the Ray Optics Regime.
    R. E. Sterba and M.P. Sheetz, Basic Laser Tweezers.
    S.C. Kuo, A Simple Assay for Local Heating by Optical Tweezers.
    A.D. Mehta, J.T. Finer, and J. Spudich, Reflections of a Lucid Dreamer: Optical Trap Design Considerations.
    M.W. Berns, Y. Tadir, H. Liang, and B. Tromberg Laser Scissors and Tweezers.
    A.L. Stout and W.W. Webb, Optical Force Microscopy.
    Y. Harada, T. Funatsu, M. Tokunaga, K. Saito, H. Higuchi, Y. Ishii, and T. Yanagida, Single Molecule Imaging and Nanomanipulation of Biomolecules.
    F. Gittes and C.F. Schmidt, Signals and Noise in Micromechanical Measurements.
    J. Dai and M.P. Sheetz, Cell Membrane Mechanics.
    A. Kusumi, Y. Sako, T. Fujiwara, and M. Tomishige, Application of Laser Tweezers to Studies of the Fences and Tethers of the Membrane Skeleton that Regulate the Movements of Plasma Membrane Proteins.
    H. Felgner, F. Grolig, O. Müller, and M. Schliwa, In Vivo Manipulation of Internal Cell Organelles.
    J.E. Molloy, Optical Chopsticks: Digital Synthesis of Multiple Optical Traps.

Product details

  • No. of pages: 228
  • Language: English
  • Copyright: © Academic Press 1997
  • Published: October 17, 1997
  • Imprint: Academic Press
  • eBook ISBN: 9780080859538

About the Serial Volume Editor

Michael Sheetz

Affiliations and Expertise

Duke University Medical Center, Durham, North Carolina, U.S.A.

About the Serial Editors

Leslie Wilson

University of California, Santa Barbara, USA

Affiliations and Expertise

University of California, Santa Barbara, USA

Paul Matsudaira

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.

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