Laser Capture in Microscopy and Microdissection, Volume 356

1st Edition

Print ISBN: 9780121822590
eBook ISBN: 9780080497013
Imprint: Academic Press
Published Date: 21st October 2002
Page Count: 775
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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Major Sections Include: @bul:* Basic Principles, Specialized Uses, and Genetic Applications

  • LCM and its application in genomics and proteomics * Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections
  • Noncontact laser catapulting for the functional genomics and proteomics
  • Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more.


Biochemists, cell and molecular biologists, geneticists, biomedical researchers, and microbiologists.

Table of Contents

Comparison of Current Equipment. Laser Capture Microdissection (LCMD) and its Applications in Genomics & Proteomics. Going in vivo With Laser MD (LMD). Use of LCMD to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis. Optimized Tissue Processing and Staining for LCMD and Nucleic Acid Retrieval. Fluoroscence in situ Hybridization of LCM-isolated Nuclei from Paraffin Sections. Immunoblotting of Single Cell Types Isolated from Frozen Sections by LMD. Noncontact Laser Catapulting: A Basic Procedure for Functl' Genomics and Proteomics. Internal Standards for LMD. Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tissues. Use of LCMD for Clonal Analysis. LCMD in Carcinoma Analysis. LCMD to Assess Devt. Application of LCMD to Proteomics. LCMD of the Mouse Intestine: Characterizing mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified. LCMD in Pathology. Use of Laser Capture Microscopy (LCM) in the Analysis of Mouse Models of Human Diseases. Use of LMD in Complex Tissue. Assessmt of Clonal Relationships in Malignant Melanomas. Comp. of Normal and Tumor Cells by LCMD. Analysis of Folliculostellate Cells by LCMD and Reverse Transcription-Polymerase Chain Reaction (LCM-RT/PCR). Analysis of Gene Expression. Analysis of Specific Gene Expression. Gene Discovery With LCM. DNA Fingerprinting from Cells Captured by LMD. Single Cell PCR in LCM. Assessmt of Genetic Clonality in Tumors using LCMD. Gene Mutations: Analysis in Proliferative Prostatic Diseases Using LCMD. Use of LCMD Generated Targets for Hybridization of Hi


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