Comparison of Current Equipment. Laser Capture Microdissection (LCMD) and its Applications in Genomics & Proteomics. Going in vivo With Laser MD (LMD). Use of LCMD to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis. Optimized Tissue Processing and Staining for LCMD and Nucleic Acid Retrieval. Fluoroscence in situ Hybridization of LCM-isolated Nuclei from Paraffin Sections. Immunoblotting of Single Cell Types Isolated from Frozen Sections by LMD. Noncontact Laser Catapulting: A Basic Procedure for Functl' Genomics and Proteomics. Internal Standards for LMD. Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tissues. Use of LCMD for Clonal Analysis. LCMD in Carcinoma Analysis. LCMD to Assess Devt. Application of LCMD to Proteomics. LCMD of the Mouse Intestine: Characterizing mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified. LCMD in Pathology. Use of Laser Capture Microscopy (LCM) in the Analysis of Mouse Models of Human Diseases. Use of LMD in Complex Tissue. Assessmt of Clonal Relationships in Malignant Melanomas. Comp. of Normal and Tumor Cells by LCMD. Analysis of Folliculostellate Cells by LCMD and Reverse Transcription-Polymerase Chain Reaction (LCM-RT/PCR). Analysis of Gene Expression. Analysis of Specific Gene Expression. Gene Discovery With LCM. DNA Fingerprinting from Cells Captured by LMD. Single Cell PCR in LCM. Assessmt of Genetic Clonality in Tumors using LCMD. Gene Mutations: Analysis in Proliferative Prostatic Diseases Using LCMD. Use of LCMD Generated Targets for Hybridization of Hi
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.
@introbul:Major Sections Include: @bul:* Basic Principles, Specialized Uses, and Genetic Applications
- LCM and its application in genomics and proteomics * Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections
- Noncontact laser catapulting for the functional genomics and proteomics
- Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more.
Biochemists, cell and molecular biologists, geneticists, biomedical researchers, and microbiologists.
- No. of pages:
- © Academic Press 2004
- 21st October 2002
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
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P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve
Texas Tech University Health Sciences Center, Lubbock, USA