Laboratory Methods in Enzymology: RNA

Laboratory Methods in Enzymology: RNA

1st Edition - September 11, 2013

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  • Author: Jon Lorsch
  • eBook ISBN: 9780124200517

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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Key Features

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on RNA


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Computational Prediction of RNA Secondary Structure

    Reverse-transcription PCR (RT-PCR)

    Northern blotting

    Explanatory Chapter: Nuclease Protection Assays

    In Vitro Transcription from Plasmid or PCR-amplified DNA

    Measurement of In Vivo RNA Synthesis Rates

    Method for measuring mRNA decay rate in Saccharomyces cerevisiae

    Analysis of polysomes from bacteria

    Polysome Profile Analysis – Yeast

    Polysome analysis of mammalian cells

    Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae

    Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates

    Circularized RT-PCR (cRT-PCR): analysis of the 5′ ends, 3′; ends, and poly(A) tails of RNA

    RNA Radiolabeling

    Fluorescently Labeling Synthetic RNAs

    Analysis of RNA by analytical polyacrylamide gel electrophoresis

    RNA purification by preparative polyacrylamide gel electrophoresis

    Explanatory Chapter: Nucleic Acid Concentration Determination

    RNA purification – precipitation methods

    Reverse Transcriptase Dideoxy Sequencing of RNA

    RNA Structure Experimental Analysis – Chemical Modification

    Structural Analysis of RNA Backbone Using In-Line Probing

Product details

  • No. of pages: 472
  • Language: English
  • Copyright: © Academic Press 2013
  • Published: September 11, 2013
  • Imprint: Academic Press
  • eBook ISBN: 9780124200517

About the Author

Jon Lorsch

Affiliations and Expertise

Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA

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