Laboratory Methods in Enzymology: Protein Part D - 1st Edition - ISBN: 9780128002797, 9780128003350

Laboratory Methods in Enzymology: Protein Part D, Volume 559

1st Edition

Laboratory Methods in Enzymology

Serial Volume Editors: Jon Lorsch
eBook ISBN: 9780128003350
Hardcover ISBN: 9780128002797
Imprint: Academic Press
Published Date: 1st February 2015
Page Count: 160
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Table of Contents

1. Purification of His-tagged Proteins
Anne Spriestersbach, Jan Kubicek, Frank Schäfer, Helena Block and Barbara Maertens
2. Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag
Krisna C. Duong-Ly and Sandra B. Gabelli
3. Immunoaffinity Purification of Proteins
Jennifer M. Kavran and Daniel J. Leahy
4. Affinity Purification of Protein Complexes Using TAP Tags
Erica Gerace and Danesh Moazed
5. Strep-tagged Protein Purification
Barbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schäfer
6. Proteolytic Affinity Tag Cleavage
Helena Block, Barbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schäfer
7. Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads
Erica Gerace and Danesh Moazed
8. Protein Affinity Purification using Intein/Chitin Binding Protein Tags
Sarah F. Mitchell and Jon R. Lorsch
9. Purification of GST-tagged Proteins
Frank Schäfer, Nicole Seip, Barbara Maertens, Helena Block and Jan Kubicek


The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters in the collection, much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few.

In this volume, number 545, we have brought together a number of core protocols concentrating on protein, carefully written and edited by experts.

Key Features

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • Brings together a number of core protocols concentrating on protein


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


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© Academic Press 2015
Academic Press
eBook ISBN:
Hardcover ISBN:

Ratings and Reviews

About the Serial Volume Editors

Jon Lorsch Serial Volume Editor

Affiliations and Expertise

Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA