Laboratory Methods in Enzymology: Protein Part D

Laboratory Methods in Enzymology: Protein Part D

Laboratory Methods in Enzymology

1st Edition - February 1, 2015
This is the Latest Edition
  • Editor: Jon Lorsch
  • eBook ISBN: 9780128003350
  • Hardcover ISBN: 9780128002797

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Description

The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters in the collection, much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. In this volume, number 545, we have brought together a number of core protocols concentrating on protein, carefully written and edited by experts.

Key Features

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • Brings together a number of core protocols concentrating on protein

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Purification of His-tagged Proteins
    Anne Spriestersbach, Jan Kubicek, Frank Schäfer, Helena Block and Barbara Maertens
    2. Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag
    Krisna C. Duong-Ly and Sandra B. Gabelli
    3. Immunoaffinity Purification of Proteins
    Jennifer M. Kavran and Daniel J. Leahy
    4. Affinity Purification of Protein Complexes Using TAP Tags
    Erica Gerace and Danesh Moazed
    5. Strep-tagged Protein Purification
    Barbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schäfer
    6. Proteolytic Affinity Tag Cleavage
    Helena Block, Barbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schäfer
    7. Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads
    Erica Gerace and Danesh Moazed
    8. Protein Affinity Purification using Intein/Chitin Binding Protein Tags
    Sarah F. Mitchell and Jon R. Lorsch
    9. Purification of GST-tagged Proteins
    Frank Schäfer, Nicole Seip, Barbara Maertens, Helena Block and Jan Kubicek

Product details

  • No. of pages: 160
  • Language: English
  • Copyright: © Academic Press 2015
  • Published: February 1, 2015
  • Imprint: Academic Press
  • eBook ISBN: 9780128003350
  • Hardcover ISBN: 9780128002797
  • About the Serial Volume Editor

    Jon Lorsch

    Affiliations and Expertise

    Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA