Laboratory Methods in Enzymology: Protein Part C, Volume 541

1st Edition

Serial Volume Editors: Jon Lorsch
Hardcover ISBN: 9780124201194
eBook ISBN: 9780124201781
Imprint: Academic Press
Published Date: 10th April 2014
Page Count: 296
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Table of Contents

  • Miscellaneous
  • Preface
  • Section I: Protein Protocols/Protein Precipitation
  • Chapter One. TCA Precipitation
    • Abstract
    • 1 Theory
    • 2 Equipment
    • 3 Materials
    • 4 Protocol
    • 5 Step 1A Trichloroacetic Acid Precipitation
    • 6 Step 1B Deoxycholate-Trichloroacetic Acid Precipitation
    • References
  • Section II: Protein Protocols/Protein Pull-Down Methods
  • Chapter Two. Coimmunoprecipitation of Proteins from Yeast
    • Abstract
    • 1 Theory
    • 2 Equipment
    • 3 Materials
    • 4 Protocol
    • 5 Step 1 Preparation of Whole Cell Lysates
    • 6 Step 2 Normalization of Cell Lysates
    • 7 Step 3 Coimmunoprecipitation
    • 8 Step 4 Wash and Elute the Immunoprecipitates
    • 9 Step 5 Analysis of Immunoprecipitations
    • References
    • Related Literature
    • Referenced Protocols in Methods Navigator
  • Chapter Three. Coupling Antibody to Cyanogen Bromide-Activated Sepharose
    • Abstract
    • 1 Theory
    • 2 Equipment
    • 3 Materials
    • 4 Protocol
    • 5 Step 1 Preparation of Antibody and Resin
    • 6 Step 2 Coupling the Antibody to the Resin
    • 7 Step 3 Quench the Reaction
    • 8 Step 4 Wash the Resin
    • References
  • Chapter Four. Analysis of Protein–Protein Interactions by Coimmunoprecipitation
    • Abstract
    • 1 Theory
    • 2 Equipment
    • 3 Materials
    • 4 Protocol
    • 5 Step 1 Isolation of the Protein of Interest by Immunoprecipitation
    • 6 Step 2 Detection of the Binding Partner by Immunoblotting
    • References
    • Referenced Protocols in Methods Navigator
  • Section III: Protein Protocols/Protein Purification
  • Chapter Five. Use and Application of Hydrophobic Interaction Chromatography for Protein Purification
    • Abstract
    • 1 Theory

Description

In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

Key Features

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on Protein

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Details

No. of pages:
296
Language:
English
Copyright:
© Academic Press 2014
Published:
Imprint:
Academic Press
eBook ISBN:
9780124201781
Hardcover ISBN:
9780124201194

About the Serial Volume Editors

Jon Lorsch Serial Volume Editor

Affiliations and Expertise

Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA