Laboratory Methods in Enzymology: DNA

Laboratory Methods in Enzymology: DNA

1st Edition - September 2, 2013
This is the Latest Edition
  • Editor: Jon Lorsch
  • eBook ISBN: 9780124199545

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Description

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Key Features

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on DNA

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Explanatory chapter: PCR Primer design

    Explanatory Chapter: How Plasmid Preparation Kits Work

    Explanatory Chapter: Introducing Exogenous DNA into cells

    Agarose Gel Electrophoresis

    Analysis of DNA by Southern Blotting

    Purification of DNA Oligos by Denaturing Polyacrylamide Gel Electrophoresis (PAGE)

    Molecular Cloning

    Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® Recombination Cloning Technology and Flp-In T-REx® lines

    Restrictionless cloning

    Isolation of plasmid DNA from bacteria

    Preparation of Genomic DNA from Bacteria

    Preparation of Genomic DNA from Saccharomyces cerevisiae

    Isolation of Genomic DNA from Mammalian Cells

    Sanger Dideoxy Sequencing of DNA

    Preparation of fragment libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD platform

    Explanatory Chapter: Next Generation Sequencing

    Generating mammalian stable cell lines by electroporation

    Transient mammalian cell Transfection with Polyethylenimine (PEI)

    Site-Directed Mutagenesis

    PCR-based random mutagenesis

    Megaprimer Method for Mutagenesis of DNA

    Explanatory Chapter: Troubleshooting PCR

    Explanatory Chapter: Quantitative PCR

    General PCR

    Colony PCR

    Chemical Transformation of Yeast

    Transformation of E. coli via electroporation

    Transformation of Chemically Competent E. coli

Product details

  • No. of pages: 408
  • Language: English
  • Copyright: © Academic Press 2013
  • Published: September 2, 2013
  • Imprint: Academic Press
  • eBook ISBN: 9780124199545

About the Serial Volume Editor

Jon Lorsch

Affiliations and Expertise

Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA