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Description
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.
In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
Key Features
- Indispensable tool for the researcher
- Carefully written and edited by experts to contain step-by-step protocols
- In this volume we have brought together a number of core protocols concentrating on DNA
Readership
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
Table of Contents
Explanatory chapter: PCR Primer design
Explanatory Chapter: How Plasmid Preparation Kits Work
Explanatory Chapter: Introducing Exogenous DNA into cells
Agarose Gel Electrophoresis
Analysis of DNA by Southern Blotting
Purification of DNA Oligos by Denaturing Polyacrylamide Gel Electrophoresis (PAGE)
Molecular Cloning
Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® Recombination Cloning Technology and Flp-In T-REx® lines
Restrictionless cloning
Isolation of plasmid DNA from bacteria
Preparation of Genomic DNA from Bacteria
Preparation of Genomic DNA from Saccharomyces cerevisiae
Isolation of Genomic DNA from Mammalian Cells
Sanger Dideoxy Sequencing of DNA
Preparation of fragment libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD platform
Explanatory Chapter: Next Generation Sequencing
Generating mammalian stable cell lines by electroporation
Transient mammalian cell Transfection with Polyethylenimine (PEI)
Site-Directed Mutagenesis
PCR-based random mutagenesis
Megaprimer Method for Mutagenesis of DNA
Explanatory Chapter: Troubleshooting PCR
Explanatory Chapter: Quantitative PCR
General PCR
Colony PCR
Chemical Transformation of Yeast
Transformation of E. coli via electroporation
Transformation of Chemically Competent E. coli
Details
- No. of pages:
- 408
- Language:
- English
- Copyright:
- © 2013
- Published:
- 23rd September 2013
- Imprint:
- Academic Press
- eBook ISBN:
- 9780124199545
- Print ISBN:
- 9780124186873
About the serial-volume-editor
Jon Lorsch
Affiliations and Expertise
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Reviews
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.
In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.