Laboratory Methods in Enzymology: DNA - 1st Edition - ISBN: 9780124186873, 9780124199545

Laboratory Methods in Enzymology: DNA, Volume 529

1st Edition

Serial Volume Editors: Jon Lorsch
eBook ISBN: 9780124199545
Hardcover ISBN: 9780124186873
Imprint: Academic Press
Published Date: 23rd September 2013
Page Count: 408
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Table of Contents

Explanatory chapter: PCR Primer design

Explanatory Chapter: How Plasmid Preparation Kits Work

Explanatory Chapter: Introducing Exogenous DNA into cells

Agarose Gel Electrophoresis

Analysis of DNA by Southern Blotting

Purification of DNA Oligos by Denaturing Polyacrylamide Gel Electrophoresis (PAGE)

Molecular Cloning

Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® Recombination Cloning Technology and Flp-In T-REx® lines

Restrictionless cloning

Isolation of plasmid DNA from bacteria

Preparation of Genomic DNA from Bacteria

Preparation of Genomic DNA from Saccharomyces cerevisiae

Isolation of Genomic DNA from Mammalian Cells

Sanger Dideoxy Sequencing of DNA

Preparation of fragment libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD platform

Explanatory Chapter: Next Generation Sequencing

Generating mammalian stable cell lines by electroporation

Transient mammalian cell Transfection with Polyethylenimine (PEI)

Site-Directed Mutagenesis

PCR-based random mutagenesis

Megaprimer Method for Mutagenesis of DNA

Explanatory Chapter: Troubleshooting PCR

Explanatory Chapter: Quantitative PCR

General PCR

Colony PCR

Chemical Transformation of Yeast

Transformation of E. coli via electroporation

Transformation of Chemically Competent E. coli


Description

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Key Features

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on DNA

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Details

No. of pages:
408
Language:
English
Copyright:
© Academic Press 2013
Published:
Imprint:
Academic Press
eBook ISBN:
9780124199545
Hardcover ISBN:
9780124186873

About the Serial Volume Editors

Jon Lorsch Serial Volume Editor

Affiliations and Expertise

Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA