Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate, Volume 533

1st Edition

Hardcover ISBN: 9780124200678
eBook ISBN: 9780124200944
Imprint: Academic Press
Published Date: 24th September 2013
Page Count: 408
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Table of Contents

Series Page

Contributors

Miscellaneous

Preface

Methods in Enzymology

Section I: Cell Protocols: Basic Microbiological Techniques

Chapter One. Pouring Agar Plates and Streaking or Spreading to Isolate Individual Colonies

Abstract

1 Theory

2 Equipment

3 Materials

4 Protocol

5 Step 1 Preparation of LB-Agar Solution

6 Step 2 Pouring Agar Plates

7 Step 3 Streaking for Individual Colonies

8 Step 4 Spreading Cells on Agar Plates

References

Chapter Two. Storage of Bacteria and Yeast

Abstract

1 Theory

2 Equipment

3 Materials

4 Protocol

5 Protocol A Cryopreservation of Bacterial Cultures

6 Step 1A Grow Bacterial Cultures

7 Step 2A Freeze the Bacterial Cells

8 Protocol B Cryopreservation of Yeast

9 Step 1B Grow Yeast Cultures

10 Step 2B Freeze the Yeast Cells

References

Section II: Cell Protocols: Cellular Fractionation

Chapter Three. Cellular Fractionation – Mammalian Cells

Abstract

1 Theory

2 Equipment

3 Materials

4 Protocol

5 Step 1 Subcellular Mammalian Cell Fractionation

Source References

Chapter Four. Cellular Fractionation – Yeast Cells

Abstract

1 Theory

2 Equipment

3 Materials

4 Protocol

5 Step 1 Yeast Protoplast Formation

6 Step 2 Subcellular Fractionation of Yeast Protoplasts

Source References

Section III: Cell Protocols: Knock-outs and Gene

Chapter Five. Yeast-Gene Replacement Using PCR Products

Abstract

1 Theory

2 Equipment

3 Materials

4 Protocol

5 Step 1 Generation of a Linear Integrating Construct by PCR

6 Step 2 Visualization of Product on an Agarose Gel

7 Step 3 Transforming Yeast with a Linear PC


Description

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Key Features

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Details

No. of pages:
408
Language:
English
Copyright:
© Academic Press 2013
Published:
Imprint:
Academic Press
eBook ISBN:
9780124200944
Hardcover ISBN:
9780124200678

Reviews

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.