Description

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Key Features

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

Series Page

Contributors

Miscellaneous

Preface

Methods in Enzymology

Section I: Cell Protocols: Basic Microbiological Techniques

Chapter One. Pouring Agar Plates and Streaking or Spreading to Isolate Individual Colonies

Abstract

1 Theory

2 Equipment

3 Materials

4 Protocol

5 Step 1 Preparation of LB-Agar Solution

6 Step 2 Pouring Agar Plates

7 Step 3 Streaking for Individual Colonies

8 Step 4 Spreading Cells on Agar Plates

References

Chapter Two. Storage of Bacteria and Yeast

Abstract

1 Theory

2 Equipment

3 Materials

4 Protocol

5 Protocol A Cryopreservation of Bacterial Cultures

6 Step 1A Grow Bacterial Cultures

7 Step 2A Freeze the Bacterial Cells

8 Protocol B Cryopreservation of Yeast

9 Step 1B Grow Yeast Cultures

10 Step 2B Freeze the Yeast Cells

References

Section II: Cell Protocols: Cellular Fractionation

Chapter Three. Cellular Fractionation – Mammalian Cells

Abstract

1 Theory

2 Equipment

3 Materials

4 Protocol

5 Step 1 Subcellular Mammalian Cell Fractionation

Source References

Chapter Four. Cellular Fractionation – Yeast Cells

Abstract

1 Theory

2 Equipment

3 Materials

4 Protocol

5 Step 1 Yeast Protoplast Formation

6 Step 2 Subcellular Fractionation of Yeast Protoplasts

Source References

Section III: Cell Protocols: Knock-outs and Gene

Chapter Five. Yeast-Gene Replacement Using PCR Products

Abstract

1 Theory

2 Equipment

3 Materials

4 Protocol

5 Step 1 Generation of a Linear Integrating Construct by PCR

6 Step 2 Visualization of Product on an Agarose Gel

7 Step 3 Transforming Yeast with a Linear PC

Details

No. of pages:
408
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Print ISBN:
9780124200678
Electronic ISBN:
9780124200944