Laboratory Methods in Cell Biology, Volume 112

1st Edition

Biochemistry and Cell Culture

Serial Volume Editors: P. Michael Conn
Hardcover ISBN: 9780124059146
eBook ISBN: 9780124055490
Imprint: Academic Press
Published Date: 26th November 2012
Page Count: 544
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Table of Contents

Series Editors

Contributors

Series Page

Preface

Chapter 1. Extraction/Characterization of DNA

1 Purpose

2 Theory

3 Equipment (in order of appearance)

4 Materials (in order of appearance)

5 Protocol

References

Chapter 2. Co-Immunoprecipitation

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 3. Real-Time PCR

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Prepare Workspace

6 Protocol

References

Chapter 4. Single-Cell Gel Electrophoresis Combined with Lesion-Specific Enzymes to Measure Oxidative Damage to DNA

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 5. High-Throughput Methods for the Identification of Protein Purification Conditions Using a Cleavable Tag System

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 6. Two-Dimensional Difference Gel Electrophoresis (2D DIGE)

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 7. Optimization of Cell Cycle Measurement by Time-Lapse Microscopy

1 Purpose

2 Theory

3 Determination of Cell Cycle Length

4 Equipments

5 Materials

6 Protocol

References

Chapter 8. Transfection Methods

1 Purpose

2 Theory

3 Equipment

4 Materials

5 Protocol

References

Chapter 9. Cell Enrichment from Human Blood through Red Cell Lysis

1 Purpose

2 Theory

3 Equipments

4 Materials

5 Protocol

References

Chapter 10. Magnetic, Batch Separation

1 Purpose

2 Theory

3 Equipment

4 Materials


Description

Cell biology spans among the widest diversity of methods in the biological sciences.  From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation.

Key Features

  • Covers the most important laboratory methods in cell biology
  • Chapters written by experts in their fields

Readership

Researchers and students in cell, molecular and developmental biology


Details

No. of pages:
544
Language:
English
Copyright:
© Academic Press 2012
Published:
Imprint:
Academic Press
eBook ISBN:
9780124055490
Hardcover ISBN:
9780124059146

Reviews

Cell biology spans among the widest diversity of methods in the biological sciences.  From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation.


About the Serial Volume Editors

P. Michael Conn Serial Volume Editor

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA