Description

Cell biology spans among the widest diversity of methods in the biological sciences.  From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone.

Key Features

    • Covers the most important laboratory methods in cell biology
    • Chapters written by experts in their fields

    Readership

    Researchers and students in cell, molecular and developmental biology

    Table of Contents

    Series Editors

    Contributors

    Dedication

    Preface

    Chapter 1. Structural Dynamics of Nucleic Acids by Single-Molecule FRET

    1 Purpose

    2 Theory

    3 Equipments

    4 Materials

    5 Protocol 1—SMFRET in Sealed Sample Chamber

    6 Protocol 2—Flow Cell for SMFRET

    References

    Chapter 2. Visualizing the Location and the Dynamics of Gene Expression in Living Animals through Bioluminescence Imaging

    1 Purpose

    2 Theory

    3 Equipment

    4 Materials

    5 Protocol

    References

    Chapter 3. Quality Control Protocol for Confocal Systems

    1 Purpose

    2 Theory

    3 Equipments

    4 Materials

    5 Protocol

    References

    Chapter 4. Electron Microscopy of Bone

    1 Purpose

    2 Theory

    3 Equipments

    4 Materials

    5 Protocol

    References

    Chapter 5. Immunostaining

    1 Purpose

    2 Theory

    3 Equipment

    4 Materials

    5 Protocol

    References

    Chapter 6. Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein–Protein Interaction

    1 Purpose

    2 Theory

    3 Equipments

    4 Materials

    5 Protocol

    References

    Chapter 7. Immunohistochemical Detection of Neuropeptides/Transmitters in Mammalian Brain

    1 Purpose

    2 Theory

    3 Equipments

    4 Materials

    5 Protocol

    References

    Chapter 8. Intravital Imaging of Autoreactive T Cells in Living Animals

    1 Purpose

    2 Theory

    3 Equipments

    4 Materials

    5 Protocol

    References

    Chapter 9. Genetically Encoded Click Chemistry for Single-Molecule FRET of Proteins

    1 Purpose

    2 Theory

    3 Equipments

    4 Materials

    5 Protocol

    References

    Chapter 10. High-Throughput Methods for the Detection of Protein Ov

    Details

    No. of pages:
    464
    Language:
    English
    Copyright:
    © 2013
    Published:
    Imprint:
    Academic Press
    Print ISBN:
    9780124072398
    Electronic ISBN:
    9780124078079