Isotope is Biology is a six-chapter supplementary text that covers the properties and application of isotopes as labels or analytical tools in biological research.
The first chapters deal with the physico-chemical properties and radioactivity of isotopes. These chapters also explore their synthesis, preparation, radiation decomposition, and decay of radioactivity. The succeeding chapter considers other aspects of isotopes, including their effect of health, disposal, spills, and laboratory use. Another chapter examines the chemical and biochemical behavior, natural abundance, and the chemical stability of isotopic compounds. The final chapters describe several isotopic methods, namely, isotope dilution, paper chromatography, and autoradiography, with emphasis on their application in biological studies. This book will be of value to biologists, and graduate and undergraduate biology students.
Preface Introduction1. What are Isotopic Tracers and Why Use Them? 2. Historical References General References
I. Some Physics and Chemistry1. The Structure of Matter; Isotopes 2. Radioactivity 3. Energy of Radiation 4. Decay of Radioactivity 5. Production of Isotopes 6. Measurement of Radioactivity and of Stable Isotope Concentration 7. Synthesis of Radioactive Compounds 8. Preparation of Tritium-Labeled Compounds 9. Preparative Biosynthesis 10. Radiation Decomposition References
II. Units of Radioactivity and of Stable Isotopes1. Units of Radioactivity; Parameters 2. Specific Radioactivity 3. Specific Activity per Mole 4. Specific Activity per Atom of Carbon 5. Units of Stable Isotopes References
III. Health Hazards and Isotope Laboratory Design1. Health Hazards 2. Radiation Protection 3. Waste Disposal 4. Spills 5. The Isotope Laboratory References
IV. Principles and Conditions for the Use of Isotopes in Biology Section I. Identical Chemical and Biochemical Behavior of Isotopic Compounds A. Cases of Nonidentical Chemical Behavior B. Cases of Nonidentical Biological Behavior Section II. Constant Natural Abundance1. Radiocarbon Dating Section III. Chemical Stability of Isotopic Compounds 1. Calcium Absorption; The Intestinal Loop References
V. Methods Section I. The Isotope Dilution Method A. The Isotope Dilution Method in the Isolation, Purification, and Characterization of Unknown Compounds B. Isotope Dilution as a Means for Studying The Synthesis of Knoton Compounds C. Isotope Dilution as An Analytical Tool Section II. Paper Chromatography and Autoradiography1. Radioactive Compounds Separated by Paper Chromatography 2. Tissue Autoradiography 3. Cel
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- © Academic Press 1964
- 1st January 1964
- Academic Press
- eBook ISBN: