iPSCs in Tissue Engineering

iPSCs in Tissue Engineering

1st Edition - August 12, 2021

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  • Editor: Alexander Birbrair
  • eBook ISBN: 9780128241905
  • Paperback ISBN: 9780128238097

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The series Advances in Stem Cell Biology is a timely and expansive collection of comprehensive information and new discoveries in the field of stem cell biology. iPSCs in Tissue Engineering, Volume 11 addresses how induced pluripotent stem cells (iPSCs) are being used to advance tissue engineering. Somatic cells can be reprogrammed into iPSCs by the expression of specific transcription factors. These cells have been transforming biomedical research over the last 15 years. This book will address the advances in research of how iPSCs are being used for the generation of different tissues and organs such as the lungs, trachea, salivary glands, skeletal muscle, liver, intestine, kidney, even the brain, and much more. This volume is written for researchers and scientists interested in stem cell therapy, cell biology, regenerative medicine, and tissue engineering and is contributed by world-renowned authors in the field.

Key Features

  • Provides overview of the fast-moving field of stem cell biology and function, regenerative medicine, and therapeutics
  • Covers the engineering of the following organs: lungs, trachea, salivary glands, skeletal muscle, liver, intestine, kidney, even the brain, and more
  • Is contributed from stem cell leaders around the world


Researchers and scientists in stem cell therapy, cell biology, regenerative medicine, and organ transplantation Graduate and undergraduate students in the above fields

Table of Contents

  • Cover image
  • Title page
  • Table of Contents
  • Advances in Stem Cell Biology
  • Copyright
  • Dedication
  • Contributors
  • About the editor
  • Preface
  • Acknowledgment
  • Chapter 1. Pluripotent stem cell–derived brain-region-specific organoids
  • Introduction
  • Methodologies to generate and culture brain-region-specific organoids
  • Applications of brain-region-specific organoids
  • Future directions
  • Chapter 2. The construction of 3D cognitive networks from iPSCs through precise spatiotemporal specification
  • Introduction
  • Origins and the molecular identity of cortical interneurons (CINs)
  • The functional classification of CINs
  • The spatiotemporal cues for CIN specification reported in rodent models
  • Human iPSCs as a model to monitor cortical interneuron development
  • The unified approach in generating hPSC-differentiated MGE in vitro
  • Monitoring temporal changes with hiPSCs’ single-cell RNA-seq
  • PSC-induced organoids as a model for CIN migration
  • Limitations in adopting hPSC-derived organoid for modeling CINs
  • Chapter 3. Induced pluripotent stem cells for vascular tissue engineering
  • Induced pluripotent stem cells—a cell source with great potential
  • Cell sources applicable for reprogramming
  • Reprogramming somatic cells into iPSCs
  • Tissue engineering
  • Vascular tissue engineering
  • Scaffolds
  • Cell source for vascular tissue engineering
  • IPSCs in vascular tissue engineering
  • Differentiating iPSCs into vascular phenotypes
  • Future outlook
  • Chapter 4. Induced pluripotent stem-cell-derived corneal grafts and organoids
  • Introduction
  • Corneal structure and function
  • Corneal development and maintenance
  • Limbal stem cells (LSCs)
  • Limbal stem cell deficiency (LSCD)
  • Therapeutic strategies for the treatment of LSCD
  • Conjunctival limbal autografts (CLAU)
  • Cultured limbal epithelial transplantations (CLET)
  • Simple limbal epithelial cell transplantations (SLET)
  • Alternative strategies for the treatment of bilateral limbal stem cell deficiency
  • Induced pluripotent stem cells and their importance in ocular research and regenerative medicine
  • Directed differentiation of iPSCs into eye field clusters and corneal specification
  • iPSC-derived three-dimensional corneal organoids and their characteristics
  • iPSC-derived corneal epithelial grafts for regenerative applications
  • iPSC-derived corneal tissues for disease modeling and in vitro drug testing
  • Major challenges in using iPSCs derived corneal cells and tissues for regeneration
  • Future perspectives and conclusion
  • Chapter 5. Induced pluripotent stem cell–derived salivary glands
  • Introduction
  • Main text
  • Future directions
  • Conclusion
  • Chapter 6. Induced pluripotent stem cells for trachea engineering
  • Introduction
  • Trachea structure and mechanical properties
  • Scaffolds for trachea engineering
  • Cell sources for trachea engineering
  • Summary
  • Chapter 7. Looking back, moving forward: the renaissance, applications, and vascularization strategies of human-induced pluripotent stem cell–derived lung organoids
  • Introduction
  • The human lung
  • Human lung organoids
  • Back to the future: strategies for vascularization
  • Conclusions and perspectives on current challenges
  • Chapter 8. Skeletal muscle engineering using human induced pluripotent stem cells for in vitro disease modeling
  • Introduction
  • Human iPSCs as a promising tool for skeletal muscle modeling
  • Patient-derived iPSCs to simulate cellular pathology in skeletal muscle in vitro
  • Derivation of myogenic progenitors from human iPSCs
  • Sphere-based expansion of myogenic progenitors from human iPSCs
  • Two-dimensional (2D) micropatterned culture for modeling human skeletal myotubes
  • Three-dimensional artificial muscle to facilitate the formation of elongated myofibrils
  • Challenges to improve in vitro muscle modeling using patient-specific iPSCs
  • Conclusion
  • Chapter 9. iPSC bioprinting for musculoskeletal tissue
  • Introduction
  • How to differentiate iPSCs into musculoskeletal tissue cells
  • iPSC myogenic differentiation
  • iPSC chondrogenic differentiation
  • iPSC osteogenic differentiation
  • Bioprinting techniques
  • Bioprinting skeletal muscle
  • Cartilage and bone bioprinting
  • Bioprinting for cartilage tissue
  • Bioprinting for bone tissue
  • Conclusions
  • Chapter 10. IPSC-derived 3D human fatty liver models
  • Liver tissue
  • Human liver cell cultures
  • Liver disease models using hiPSC-Hep
  • Current status and future perspectives
  • Chapter 11. IPSC-derived intestinal organoids and current 3D intestinal scaffolds
  • Introduction to the intestinal tissue morphology
  • Organoid limitations
  • Ideal intestinal scaffold requirements
  • Current state-of-the-art in in vitro intestinal tissue models
  • Future perspectives
  • Chapter 12. Models of kidney glomerulus derived from human-induced pluripotent stem cells
  • Introduction
  • The glomerulus in the context of the nephron: basic anatomy and physiology
  • Modeling the glomerulus: insights from the embryological development
  • Modeling kidney development in vitro: generation of kidney organoids
  • Modeling glomerular development and function in vitro: glomerulus-on-a-chip and bioprinting
  • Future perspectives: innovations in bioengineering of kidney tissues from iPSCs
  • Chapter 13. Ureteric bud structures generated from human iPSCs
  • Introduction
  • Early studies generating UB-lineage cells
  • Establishment of differentiation methods for UB tissues
  • Generation of a disease model using iUB organoids
  • Hurdles to overcome
  • Index

Product details

  • No. of pages: 430
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: August 12, 2021
  • Imprint: Academic Press
  • eBook ISBN: 9780128241905
  • Paperback ISBN: 9780128238097

About the Editor

Alexander Birbrair

Dr. Alexander Birbrair received his bachelor’s biomedical degree from Santa Cruz State University in Brazil. He completed his PhD in Neuroscience, in the field of stem cell biology, at the Wake Forest School of Medicine under the mentorship of Osvaldo Delbono. Then, he joined as a postdoc in stem cell biology at Paul Frenette’s laboratory at Albert Einstein School of Medicine in New York. In 2016, he was appointed faculty at Federal University of Minas Gerais in Brazil, where he started his own lab. His laboratory is interested in understanding how the cellular components of different tissues function and control disease progression. His group explores the roles of specific cell populations in the tissue microenvironment by using state-of-the-art techniques. His research is funded by the Serrapilheira Institute, CNPq, CAPES, and FAPEMIG. In 2018, Alexander was elected affiliate member of the Brazilian Academy of Sciences (ABC), and, in 2019, he was elected member of the Global Young Academy (GYA), and in 2021, he was elected affiliate member of The World Academy of Sciences (TWAS). He is the Founding Editor and Editor-in-Chief of Current Tissue Microenvironment Reports, and Associate Editor of Molecular Biotechnology. Alexander also serves in the editorial board of several other international journals: Stem Cell Reviews and Reports, Stem Cell Research, Stem Cells and Development, and Histology and Histopathology.

Affiliations and Expertise

Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil Department of Radiology, Columbia University Medical Center, Medical Center, USA

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  • Dillon Sat Mar 26 2022

    Very informative!

    I really enjoyed it, very informative, highly recommend it!