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Ion Channels - 1st Edition - ISBN: 9780121821081, 9780080925493

Ion Channels, Volume 207

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editors: Bernardo Rudy Linda Iverson
eBook ISBN: 9780080925493
eBook ISBN: 9780080883212
Imprint: Academic Press
Published Date: 27th July 1992
Page Count: 917


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Table of Contents

Contributors. Preface. Volumes in Series. Section I: Modern Applications of Electrophysiological Techniques: Patch Clamp Techniques: An Overview, M. Cahalan and E. Neher. Constructing a Patch Clamp Setup, R.A. Levis and J.L. Rae. Glass Technology for Patch Clamp Electrodes, J.L. Rae and R.A. Levis. Ion Channel Selectivity, Permeation, and Block, T. Begenisich. Access Resistance and Space Clamp Problems Associated with Whole-Cell Patch Clamping, C.M. Armstrong and W.F. Gilly. Correction for Liquid Junction Potentials in Patch Clamp Experiments, E. Neher. Nonstationary Noise Analysis and Application to Patch Clamp Recordings, S.H. Heinemann and F. Conti. Prevention of Rundown in Electrophysiological Recording, R. Horn and S.J. Korn. Patch Voltage Clamping with Low-Resistance Seals: Loose Patch Clamp, W.M. Roberts and W. Almers. Perfusing Patch Pipettes, J.M. Tang, J. Wang, and R.S. Eisenberg. Whole-Cell Recording of Calcium Channel Currents, B.P. Bean. Recording from Calcium-Activated Potassium Channels, B.S. Pallotta, A.L. Blatz and K.L. Magleby. Patch-Clamping Cells in Sliced Tissue Preparations, F.A. Edwards and A. Konnerth. Section II. Expression of Ion Channels: A.: Expression of Ion Channels in Xenopus Oocytes: Xenopus Oocyte Microinjection: From Gene to Protein, H. Soreq and S. Seidman. Maintenance of Xenopus laevis and Oocyte Injection, A.L. Goldin. Preparation of RNA for Injection into Xenopus Oocytes, A.L. Goldin and K. Sumikawa. Tissue RNA as Source of Ion Channels and Receptors, T.P. Snutch and G. Mandel. In Vitro Synthesis of RNA for Expression of Ion Channels in Xenopus Oocytes, T. Snutch and G. Mandel. Electrophysiological Recording from Xenopus Oocytes, W. Stühmer. Use of Stage II-III Xenopus Oocytes to Study Voltage-Dependent Ion Channels, D.S. Krafte and H.A. Lester. Intracellular Perfusion of Xenopus Oocytes, N. Dascal, G. Chilcott, and H.A. Lester. Recording of Gating Currents from Xenopus Oocytes and Gating Noise Analysis, S.H. Heinemann, F. Conti, and W. Stühmer. Ligand-Binding Assays in Xenopus Oocytes, A.L. Buller and M.M. White. Expression of Gap Junctional Proteins in Xenopus Oocyte Pairs, L. Ebihara. Regulation of Intracellular Calcium Activity in Xenopus Oocytes, Y. Oron and N. Dascal. B. Expression of Ion Channels Using Other Systems: Stable Expression of Heterologous Multisubunit Protein Complexes Established by Calcium Phosphate- or Lipid-Mediated Cotransfection, T. Claudio. Vaccinia Virus as Vector to Express Ion Channel Genes, A. Karschin, B.A. Thorne, G. Thomas, and H.A. Lester. Expression of Ion Channels in Cultured Cells Using Baculovirus, A. Kamb, J.I. Korenbrot, and J. Kitajewski. Probing Molecular Structure and Structural Changes of Voltage-Gated Channel by Expressing Mutant Channels in Yeast and Reconstituting Them into Planar Membranes, M. Colombini, S. Peng, E. Blachly-Dyson, and M. Forte. Section III. Reconstitution of Ion Channels in Lipid Bilayers: Insertion of Ion Channels into Planar Lipid Bilayers by Vesicle Fusion, P. Labarca and R. Latorre. Planar Lipid Bilayers on Patch Pipettes: Bilayer Formation and Ion Channel Incorporation, B.E. Ehrlich. Surface Charge Effects on Ion Conduction in Ion Channels, R. Latorre, P. Labarca, and D. Naranjo. Determination of Ion Permeability by Fluorescence Quenching, A.M. Garcia. Synthetic Peptides and Proteins as Models for Pore-Forming Structure of Channel Proteins, A. Grove, T. Iwamoto, M.S. Montal, J.M. Tomich, and M. Montal. Section IV. Purification of Ion Channel Proteins and Genes: Purification and Reconstitution of Skeletal Muscle Calcium Channels, V. Florio, J. Striessnig, and W.A. Catterall. Purification and Reconstitution of Nicotinic Acetylcholine Receptor, A. Chak and A. Karlin. Purification, Affinity Labeling, and Reconstitution of a Voltage-Sensitive Potassium Channels, H. Rehm and M. Lazudunski. Affinity Purification and Reconstitution of Calcium-Activated Potassium Channels, D.A. Klaerke and P.L. J#248;rgensen. Assay and Purification of Neuronal Receptors for Inositol 1,4,5-Triphosphate, S.R. Hingorani and W.S. Agnew. Isolation of Ion Channel Genes by Expression Cloning in Xenopus Oocytes, G.C. Frech and R.H. Joho. Hybrid Arrest Technique to Test for Functional Roles of Cloned cDNAs and to Identify Homologies among Ion Channel Genes, I. Lotan. Cloning of Ion Channel Gene Families Using the Polymerase Chain Reaction, E. Vega-Saenz De Miera and J.-W. Lin. Overview of Toxins and Drugs as Tools to Study Excitable Membrane Ion Channels I: Voltage-Activated Channels, T. Narahashi and M.D. Herman. Overview of Toxins and Drugs as Tools to Study Excitable Membrane Ion Channels II: Transmitter-Activated Channels, T. Narahashi. Biosynthesis of Ion Channels in Cell-Free and Metabolically Labeled Cell Systems, W.B. Thornhill and S.R. Levinson. Section V. Recording of Ion Channels of Cellular Organelles and Microorganisms: Patch Clamp Techniques in Study of Ion Channels from Organelles, B.U. Keller and R. Hedrich. Patch Clamp Studies of Microbial Ion Channels, Y. Saimi, B. Martinac, A.H. Delcour, P.V. Minorsky, M.C. Gustin, M.R. Culbertson, J. Adler, and C. Kung. Studies on Intact Sarcoplasmic Reticulum: Patch Clamp Recording and Tension Measurement in Lobster Split Muscle Fibers, J.M. Tang, J. Wang, and R.S. Eisenberg. Planar Bilayer Recording of Ryanodine Receptors of Sarcoplasmic Reticulum, R. Coronado, S. Kawano, C.J. Lee, C. Valdivia, and H.H. Valdivia. Section VI. Data Storage and Analysis: Software for Acquisition and Analysis of Ion Channel Data: Choices, Tasks, and Strategies, R.J. French and W.F. Wonderlin. Stationary Single-Channel Analysis, M.B. Jackson. Analysis of Nonstationary Single-Channel Currents, F.J. Sigworth and J. Zhou. Preventing Artifacts and Reducing Errors in Single-Channel Analysis, K.L. Magleby. Analysis of Drug Action at Single-Channel Level, E. Moczydlowski. Analysis of Sodium Channel Tail Currents, G. Cota and C.M. Armstrong. Calculation of Ion Currents from Energy Profiles and Energy Profiles from Ion Currents in a Multibarrier, Multisite, Multioccupancy Channel Model, O. Alvarez, A. Villarroel, and G. Eisenman. Author Index. Subject Index.


This book describes modern electrophysiological techniques for recording and analysis of ion channels in various systems including cells in culture, Xenopus oocytes, brain slices, cellular organelles, and microorganisms. Many chapters deal with the prevention and correction of electrophysiological artifacts. Also included are methods for the purification and expression of ion channel genes and proteins.

Key Features

@introbul:Key Features @bul:* Applications of electrophysiological techniques

  • Expression in heterologous systems
  • Reconstitution of lipid bilayers
  • Purification and characterization of ion channel proteins and genes
  • Recording of ion channels of intracellular membranes and microorganisms
  • Data storage and analysis


Biochemists, cell biologists, molecular biologists, physiologists, geneticists, biophysicists, neurophysiologists, microbiologists, and pharmacologists.


No. of pages:
© Academic Press 1992
27th July 1992
Academic Press
eBook ISBN:
eBook ISBN:


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About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

Bernardo Rudy

Affiliations and Expertise

New York University Medical Center, New York, U.S.A.

Linda Iverson

Affiliations and Expertise

Beckman Research Institute of the City of Hope, Duarte, California, U.S.A.