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Ion Channels, Part C - 1st Edition - ISBN: 9780121821951, 9780080884059

Ion Channels, Part C, Volume 294

1st Edition

Serial Volume Editor: P. Michael Conn
Editors in Chief: John Abelson Melvin Simon
eBook ISBN: 9780080884059
Imprint: Academic Press
Published Date: 2nd October 1998
Page Count: 788


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Table of Contents

Contributors to Volume 294. Preface. Volumes in Series. Physical Methods: Optical Measurements of Calcium Signals in Mammalian Presynaptic Terminals, P. Saggau, R. Gray and J.A. Dani. Fluorescent Techniques for Measuring Ion Channel Activity, G. Velicelebi, K.A. Strauderman, M.A. Varney, M. Akong, S.D. Hess and E.C. Johnson. Tagging Potassium Ion Channels with Green Fluorescent Protein to Study Mobility and Interactions with Other Proteins, E.S. Levitan. Spin Label Electron Spin Resonance and Fourier Transform Spectroscopy for Structural/Dynamic Measurements on Ion Channels, D. Marsh. Nuclear Magnetic Resonance Spectroscopy of Peptide Ion Channel Ligands: Cloning and Expression as Aid to Evaluation of Structural and Dynamic Properties, M.D. Reily, A.M. Bokman, J. Offord and P. McConnell. Ligand Binding Methods for Analysis of Ion Channel Structure and Function, S.E. Pedersen, M.M. Lurtz and R.V.L. Papineni. Three-Dimensional Structure of Membrane Proteins Determined by Two-Dimensional Crystallization, Electron Cryomicroscopy, and Image Analysis, M. Yeager, V.M. Unger and A.K. Mitra. Drug-Dependent Ion Channel Gating by Application of Concentration Jumps Using U-Tube Technique, F. Bretschneider and F. Markwardt. Voltage Clamp Biosensors for Capillary Electrophoresis, O. Orwar, K. Jardemark, C. Farre, I. Jacobson, A. Moscho, J.B. Shear, H.A. Fishman, S.J. Lillard and R.N. Zare. Ion Channels as Tools to Monitor Lipid Bilayer-Membrane Protein Interactions: Gramicidin Channels as Molecular Force Transducers, O.S. Andersen, C. Nielsen, A.M. Maer, J.A. Lundbaek, M. Goulian and R.E. Koeppe, III. Purification and Reconstitution: Purification and Reconstitution of Epithelial Chloride Channel Cystic Fibrosis Transmembrane Conductance Regulator, M. Ramjeesingh, E. Garami, L.-J. Huan, K. Galley, C. Li, Y. Wang and C.E. Bear. Purification, Characterization, and Reconstitution of Cyclic Nucleotide-Gated Channels, R.S. Molday and L.L. Molday. Purification and Heterologous Expression of Inhibitory Glycine Receptors, B. Laube and H. Betz. Purification and Functional Reconstitution of High-Conductance Calcium-Activated Potassium Channel from Smooth Muscle, M.L. Garcia, K.M. Giangiacomo, M. Hanner, H.-G. Knaus, O.B. McManus, W.A. Schmalhofer and G.J. Kaczorowski. Reconstitution of Native and Cloned Channels into Planar Bilayers, I. Favre, Y.-M. Sun and E. Moczydlowski. Iodide Channel of the Thyroid: Reconstituting Ion Channels into Planar Lipid Bilayers, P.E. Golstein, A. Sener, F. Colin and R. Beauwens. Nystatin/Ergosterol Method for Reconstituting Ion Channels into Planar Lipid Bilayers, D.J. Woodbury. Isolation of Transport Vesicles That Deliver Ion Channels to the Cell Surface, S. Sattsangi and W.F. Wonderlin. Second Messengers and Biochemical Approaches: Protein Phosphorylation of Ligand-Gated Ion Channels, A.L. Mammen, S. Kamboj and R.L. Huganir. Analysis of Ion Channel Associated Proteins, M. Wyszynski and M. Sheng. Signal Transduction through Ion Channels Associated with Excitatory Amino Acid Receptors, K. Ogita and Y. Yoneda. Secondary Messenger Regulation of Ion Channels/Plant Patch Clamping, S.M. Assmann and L. Romano. Special Channels: ATP-Sensitive Potassium Channels, M. Schwanstecher, C. Schwanstecher, F. Chudziak, U. Panten, J.P. Clement IV, G. Gonzalez, L. Aguilar-Bryan and J. Bryan. Mechanosensitive Channels of Bacteria, P. Blount, S.I. Sukharev, P.C. Moe, B. Martinac and C. Kung. Simplified Fast Pressure-Clamp Technique for Studying Mechanically Gated Channels, D.W. McBride, Jr. and O.P. Hamill. Virus Ion Channels, D.C. Ogden, I.V. Chizhmakov, F.M. Geraghty and A.J. Hay. Ion Channels in Microbes, Y. Saimi, S.H. Loukin, X.-L. Zhou, B. Martinac and C. Kung. Design and Characterization of Gramicidin Channels, D.V. Greathouse, R.E. Koeppe II, L.L. Providence, S. Shobana and O.S. Andersen. Functional Analyses of Aquaporin Water Channel Proteins, P. Agre, J.C. Mathai, B.L. Smith and G.M. Preston. Toxins and Other Membrane Active Compounds: Pore-Blocking Toxins as Probes of Voltage-Dependent Channels, R.J. French and S.C. Dudley, Jr.. Conus Peptides as probes for Ion Channels, J.M. McIntosh, B.M. Olivera and L.J. Cruz. Scorpion Toxins as Tools for Studying Potassium Channels, M.L. Garcia, M. Hanner, H.-G. Knaus, R. Slaughter and G.J. Kaczorowski. Potassium Ion Channel Inactivation Peptides, R.D. Murrell-Lagnado. Interactions of Snake Dendrotoxins with Potassium Channels, W.F. Hopkins, M. Allen and B.L. Tempel. Use of Planar Lipid Bilayer Membranes for Rapid Screening of Membrane Active Compounds, T.A. Mirzabekov, A.Y. Silberstein and B.L. Kagan. Reagent and Information Sources: Antibodies to Ion Channels, A.C. Vincent, I.K. Hart, A. Pinto and F.A. Stephenson. Internet Information on Ion Channels: Issues of Access and Organization, E.C. Conley. Author Index. Subject Index.


General Description of the Volume: This volume and its companion Volume 293 present techniques for the study of ion channels, ranging from the molecular to the physiological level. These volumes supplement Volume 207 of Methods in Enzymology. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Among the major topics covered in this volume are:

  • Physical methods
  • Purification and reconstitution
  • Second messengers and biochemical approaches
  • Special channels


AUDIENCE: Biochemists, neurophysiologists, molecular biologists, biophysicists, cell biologists, microbiologists, geneticists, and pharmacologists.


No. of pages:
© Academic Press 1999
2nd October 1998
Academic Press
eBook ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Serial Volume Editor

P. Michael Conn

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA