General Description of the Volume: This volume and its companion Volume 293 present techniques for the study of ion channels, ranging from the molecular to the physiological level. These volumes supplement Volume 207 of Methods in Enzymology.
General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
@introbul:Key Features @bul:* Among the major topics covered in this volume are:
- Physical methods
- Purification and reconstitution
- Second messengers and biochemical approaches
- Special channels
AUDIENCE: Biochemists, neurophysiologists, molecular biologists, biophysicists, cell biologists, microbiologists, geneticists, and pharmacologists.
Contributors to Volume 294. Preface. Volumes in Series.
Physical Methods: Optical Measurements of Calcium Signals in Mammalian Presynaptic Terminals, P. Saggau, R. Gray and J.A. Dani. Fluorescent Techniques for Measuring Ion Channel Activity, G. Velicelebi, K.A. Strauderman, M.A. Varney, M. Akong, S.D. Hess and E.C. Johnson. Tagging Potassium Ion Channels with Green Fluorescent Protein to Study Mobility and Interactions with Other Proteins, E.S. Levitan. Spin Label Electron Spin Resonance and Fourier Transform Spectroscopy for Structural/Dynamic Measurements on Ion Channels, D. Marsh. Nuclear Magnetic Resonance Spectroscopy of Peptide Ion Channel Ligands: Cloning and Expression as Aid to Evaluation of Structural and Dynamic Properties, M.D. Reily, A.M. Bokman, J. Offord and P. McConnell. Ligand Binding Methods for Analysis of Ion Channel Structure and Function, S.E. Pedersen, M.M. Lurtz and R.V.L. Papineni. Three-Dimensional Structure of Membrane Proteins Determined by Two-Dimensional Crystallization, Electron Cryomicroscopy, and Image Analysis, M. Yeager, V.M. Unger and A.K. Mitra. Drug-Dependent Ion Channel Gating by Application of Concentration Jumps Using U-Tube Technique, F. Bretschneider and F. Markwardt. Voltage Clamp Biosensors for Capillary Electrophoresis, O. Orwar, K. Jardemark, C. Farre, I. Jacobson, A. Moscho, J.B. Shear, H.A. Fishman, S.J. Lillard and R.N. Zare. Ion Channels as Tools to Monitor Lipid Bilayer-Membrane Protein Interactions: Gramicidin Channels as Molecular Force Transducers, O.S. Andersen, C. Nielsen, A.M. Maer, J.A. Lundbaek, M. Goulian and R.E. Koeppe, III.
Purification and Reconstitution: Purification and Reconstitution o
- No. of pages:
- © Academic Press 1999
- 2nd October 1998
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve
Texas Tech University Health Sciences Center, Lubbock, USA
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