General Description of the Volume: This volume and its companion Volume 293 present techniques for the study of ion channels, ranging from the molecular to the physiological level. These volumes supplement Volume 207 of Methods in Enzymology. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Among the major topics covered in this volume are: * Physical methods * Purification and reconstitution * Second messengers and biochemical approaches * Special channels


AUDIENCE: Biochemists, neurophysiologists, molecular biologists, biophysicists, cell biologists, microbiologists, geneticists, and pharmacologists.

Table of Contents

Contributors to Volume 294. Preface. Volumes in Series. Physical Methods: Optical Measurements of Calcium Signals in Mammalian Presynaptic Terminals, P. Saggau, R. Gray and J.A. Dani. Fluorescent Techniques for Measuring Ion Channel Activity, G. Velicelebi, K.A. Strauderman, M.A. Varney, M. Akong, S.D. Hess and E.C. Johnson. Tagging Potassium Ion Channels with Green Fluorescent Protein to Study Mobility and Interactions with Other Proteins, E.S. Levitan. Spin Label Electron Spin Resonance and Fourier Transform Spectroscopy for Structural/Dynamic Measurements on Ion Channels, D. Marsh. Nuclear Magnetic Resonance Spectroscopy of Peptide Ion Channel Ligands: Cloning and Expression as Aid to Evaluation of Structural and Dynamic Properties, M.D. Reily, A.M. Bokman, J. Offord and P. McConnell. Ligand Binding Methods for Analysis of Ion Channel Structure and Function, S.E. Pedersen, M.M. Lurtz and R.V.L. Papineni. Three-Dimensional Structure of Membrane Proteins Determined by Two-Dimensional Crystallization, Electron Cryomicroscopy, and Image Analysis, M. Yeager, V.M. Unger and A.K. Mitra. Drug-Dependent Ion Channel Gating by Application of Concentration Jumps Using U-Tube Technique, F. Bretschneider and F. Markwardt. Voltage Clamp Biosensors for Capillary Electrophoresis, O. Orwar, K. Jardemark, C. Farre, I. Jacobson, A. Moscho, J.B. Shear, H.A. Fishman, S.J. Lillard and R.N. Zare. Ion Channels as Tools to Monitor Lipid Bilayer-Membrane Protein Interactions: Gramicidin Channels as Molecular Force Transducers, O.S. Andersen, C. Nielsen, A.M. Maer, J.A. Lundbaek, M. Goulian and R.E. Koeppe, III. Purification and Reconstitution: Purification and Reconstitution o


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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY