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Ion Channels, Part B - 1st Edition - ISBN: 9780121821944, 9780080884042

Ion Channels, Part B, Volume 293

1st Edition

Serial Volume Editor: P. Michael Conn
Editors in Chief: John Abelson Melvin Simon
eBook ISBN: 9780080884042
Imprint: Academic Press
Published Date: 31st July 1998
Page Count: 805


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Table of Contents

Assembly: J. Xu and M. Li, Methods Used to Study Subunit Assembly of Potassium Channels. Z.F. Sheng and C. Deutsch, Assembly of Ion Channels. J.S. Trimmer, Analysis of K+ Channel Biosynthesis and Assembly in Transfected Mammalian Cells. Genetics: T.M. Ishii, P. Zerr, X-m. Xia, C.T. Bond, J. Maylie, and J.P. Adelman, Site-Directed Mutagenesis. S. Richard, P. Lory, E. Bourinet, and J. Nargeot, Molecular Physiology of Human Cardiovascular Ion Channels: From Electrophysiology to Molecular Genetics. R.L. Nakamura and R.F. Gaber, Studying Ion Channels Using Yeast Genetics. M. Tiethammer and M. Sheng, Identification of Ion Channel-Associated Proteins Using Yeast Two-Hybrid System. A. Karlin and M.H. Akabas, Substituted-Cysteine Accessibility Method. R. Horn, Explorations of Voltage-Dependent Conformational Changes Using Cysteine Scanning. K. Sato and M. Tohyama, Assessment of Distribution of Cloned Ion Channels in Neuronal Tissues. Electrophysiology: J.W. Hanrahan, Z. Kone, C.J. Mathews, J. Luo, Y. Jia, and P. Linsdell, Patch Clamp Studies of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel. T.J. Nelson, P.A. Gusev, and D.L. Alkon, Identification of Ion Channel Regulating Proteins by Patch Clamp Analysis. S.R. Lockery and M.B. Goodman, Tight-Seal Whole-Cell Patch Clamping of Caenorhabditis elegans Neurons. R.A. Levis and J.L. Rae, Low Noise Patch Clamp Techniques. D.N. Hilgemann and C.C. Lu, Giant Membrane Patches: Improvements and Applications. W. Stühmer, Electrophysiological Recordings from Xenopus Oocytes. E. Stefani and F. Bezanilla, Cut-Open Oocyte Voltage Clamp Technique. S. Kaneko, A. Akaike, and M. Satoh, Cut-Open Recording Techniques. F. Bezanilla and E. Stefani, Gating Currents. J.G. Borst and F. Helmchen, Calcium Influx During Action Potentials. L.D. Partridge, H.U. Zeilhofer, and D. Swandulla, Combined Whole-Cell and Single-Channel Current Measurement with Quantitative Ca2+ Injection or Fura-2 Measurement of Ca2+. T. Begenisich, Determining Ion Channel Permeation Properties. R. Steffan, C. Hennesthal, and S.H. Heinemann, Voltage-Dependent Ion Channels: Analysis of Nonideal Macroscopic Current Data. S.H. Chung and P.W. Gage, Signal Processing Techniques for Channel Current Analysis Based on Hidden Markov Models. B.S. Rothberg and K.L. Magleby, Investigating Single-Channel Gating Mechanisms through Analysis of Two-Dimensional Dwell-Time Distributions. Expression Systems: K. Radford and G. Buell, Expression of Ligand-Gated Ion Channels Using Semliki Forest Virus and Baculovirus. M.U. Ehrengruber, M. Lanzrein, Y. Xu, M.C. Jasek, D.B. Kantor, E.M. Schuman, H.A. Lester, and N. Davidson, Recombinant Adenovirus-Mediated Expression in Nervous System of Genes Coding for Ion Channels and Other Molecules Involved in Synaptic Function. M.W. Nowak, J.P. Gallivan, S.K. Silverman, C.G. Labarca, D.A. Dougherty, and H.A. Lester, In Vivo Incorporation of Unnatural Amino Acids into Ion Channels in Xenopus Oocyte Expression System. T.M. Shih, R.D. Smith, L. Toro, and A.L. Goldin, High-Level Expression and Detection of Ion Channels in Xenopus Oocytes. P. Stampe and T. Begenisich, Unidirectional Fluxes through Ion Channels Expressed in Xenopus Oocytes. A.L. Eertmoed, Y.F. Vallejo, and W.N. Green, Transient Expression of Heteromeric Ion Channels. Model Simulations: M.J. Sutcliffe, A.H. Smeeton, Z.G. Wo, and R.E. Oswald, Molecular Modeling of Ligand-Gated Ion Channels. S.P. Scott and J.C. Tanaka, Use of Homology Modeling to Predict Residues Involved in Ligand Recognition. M.S.P. Sansom, Ion Channels: Molecular Modeling and Simulation Studies. M.E. Green, Computer Simulation and Modeling of Ion Channels. V. Ardonin and T. Hoshi, Kinetic Models and Simulation: Practical Approaches and Implementation Notes. Subject Index. Author Index.


This volume plus its companion, Volume 294, presents cutting edge techniques for the study of ion channels, ranging from the molecular to the physiological level. These volumes supplement Volume 207 of Methods inEnzymology

Key Features

@introbul:Key Features @bul:* Assembly

  • Genetics
  • Electrophysiology
  • Expression Systems
  • Model Simulations


Biochemists, neurophysiologists, molecular biologists, pharmacologists, biophysicists, cell biologists, geneticists, and microbiologists.


No. of pages:
© Academic Press 1998
31st July 1998
Academic Press
eBook ISBN:


@from:Praise for the Volume @qu:"...the readers will enjoy to see assembled in one volume such an amount of information of the highest degree for the development of their researches." @source:--CELLULAR AND MOLECULAR BIOLOGY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Serial Volume Editor

P. Michael Conn

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA