This volume plus its companion, Volume 294, presents cutting edge techniques for the study of ion channels, ranging from the molecular to the physiological level. These volumes supplement Volume 207 of Methods inEnzymology
@introbul:Key Features @bul:* Assembly
- Expression Systems
- Model Simulations
Biochemists, neurophysiologists, molecular biologists, pharmacologists, biophysicists, cell biologists, geneticists, and microbiologists.
J. Xu and M. Li, Methods Used to Study Subunit Assembly of Potassium Channels.
Z.F. Sheng and C. Deutsch, Assembly of Ion Channels.
J.S. Trimmer, Analysis of K+ Channel Biosynthesis and Assembly in Transfected Mammalian Cells. Genetics:
T.M. Ishii, P. Zerr, X-m. Xia, C.T. Bond, J. Maylie, and J.P. Adelman, Site-Directed Mutagenesis.
S. Richard, P. Lory, E. Bourinet, and J. Nargeot, Molecular Physiology of Human Cardiovascular Ion Channels: From Electrophysiology to Molecular Genetics.
R.L. Nakamura and R.F. Gaber, Studying Ion Channels Using Yeast Genetics.
M. Tiethammer and M. Sheng, Identification of Ion Channel-Associated Proteins Using Yeast Two-Hybrid System.
A. Karlin and M.H. Akabas, Substituted-Cysteine Accessibility Method.
R. Horn, Explorations of Voltage-Dependent Conformational Changes Using Cysteine Scanning.
K. Sato and M. Tohyama, Assessment of Distribution of Cloned Ion Channels in Neuronal Tissues. Electrophysiology:
J.W. Hanrahan, Z. Kone, C.J. Mathews, J. Luo, Y. Jia, and P. Linsdell, Patch Clamp Studies of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel.
T.J. Nelson, P.A. Gusev, and D.L. Alkon, Identification of Ion Channel Regulating Proteins by Patch Clamp Analysis.
S.R. Lockery and M.B. Goodman, Tight-Seal Whole-Cell Patch Clamping of Caenorhabditis elegans Neurons.
R.A. Levis and J.L. Rae, Low Noise Patch Clamp Techniques.
D.N. Hilgemann and C.C. Lu, Giant Membrane Patches: Improvements and Applications.
W. Stühmer, Electrophysiological Recordings from Xenopus Oocytes. <
- No. of pages:
- © Academic Press 1998
- 31st July 1998
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve
Texas Tech University Health Sciences Center, Lubbock, USA
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