This volume plus its companion, Volume 294, presents cutting edge techniques for the study of ion channels, ranging from the molecular to the physiological level. These volumes supplement Volume 207 of Methods inEnzymology

Key Features

@introbul:Key Features @bul:* Assembly * Genetics * Electrophysiology * Expression Systems * Model Simulations


Biochemists, neurophysiologists, molecular biologists, pharmacologists, biophysicists, cell biologists, geneticists, and microbiologists.

Table of Contents

Assembly: J. Xu and M. Li, Methods Used to Study Subunit Assembly of Potassium Channels. Z.F. Sheng and C. Deutsch, Assembly of Ion Channels. J.S. Trimmer, Analysis of K+ Channel Biosynthesis and Assembly in Transfected Mammalian Cells. Genetics: T.M. Ishii, P. Zerr, X-m. Xia, C.T. Bond, J. Maylie, and J.P. Adelman, Site-Directed Mutagenesis. S. Richard, P. Lory, E. Bourinet, and J. Nargeot, Molecular Physiology of Human Cardiovascular Ion Channels: From Electrophysiology to Molecular Genetics. R.L. Nakamura and R.F. Gaber, Studying Ion Channels Using Yeast Genetics. M. Tiethammer and M. Sheng, Identification of Ion Channel-Associated Proteins Using Yeast Two-Hybrid System. A. Karlin and M.H. Akabas, Substituted-Cysteine Accessibility Method. R. Horn, Explorations of Voltage-Dependent Conformational Changes Using Cysteine Scanning. K. Sato and M. Tohyama, Assessment of Distribution of Cloned Ion Channels in Neuronal Tissues. Electrophysiology: J.W. Hanrahan, Z. Kone, C.J. Mathews, J. Luo, Y. Jia, and P. Linsdell, Patch Clamp Studies of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel. T.J. Nelson, P.A. Gusev, and D.L. Alkon, Identification of Ion Channel Regulating Proteins by Patch Clamp Analysis. S.R. Lockery and M.B. Goodman, Tight-Seal Whole-Cell Patch Clamping of Caenorhabditis elegans Neurons. R.A. Levis and J.L. Rae, Low Noise Patch Clamp Techniques. D.N. Hilgemann and C.C. Lu, Giant Membrane Patches: Improvements and Applications. W. Stühmer, Electrophysiological Recordings from Xenopus Oocytes. <


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© 1998
Academic Press
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@from:Praise for the Volume @qu:"...the readers will enjoy to see assembled in one volume such an amount of information of the highest degree for the development of their researches." @source:--CELLULAR AND MOLECULAR BIOLOGY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY