Ion Channels: Channel Chemical Biology, Engineering, and Physiological Function
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Ion Channels: Channel Chemical Biology, Engineering, and Physiological Function

1st Edition - June 12, 2021

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  • Editors: Daniel Minor, Henry Colecraft
  • eBook ISBN: 9780128239254
  • Hardcover ISBN: 9780128239247

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Description

Ion Channels, Part C, Volume 653 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Nonsense suppression in ion channels, Engineering Ion Channels Using Protein Trans-splicing, Probing Ion Channel Neighborhoods Using APEX, STX based probes for NaVs, ANAP: a versatile, fluorescent probe of ion channel gating and regulation, High Throughput Screens for Small Molecule Ion Channel Modulators, Using toxins to study ion channels, Re/de-constructing ubiquitin regulation of ion channels, Tethered Peptide Toxins for Ion Channels, Voltage-Sensing Phosphatase Molecular Engineering, and more. Additional chapters cover Engineering excitable cells, Stretch and Poke Stimulation of Mechanically-Activated Ion Channels, Optical Control of STIM Channels, High Throughput Electrophysiological Evaluation of Mutant Ion Channels, Evaluating BEST1 Mutations in RPE Stem Cells, Long Read Transcript Profiling of Ion Channel Splice Variants, Permeation of Connexin Channels, Ratiometric pH indicator for melanosomes and lysosomes, and Ion channels in the epithelial cells of the choroid plexus.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Preface

    Daniel L. Minor Jr. and Henry M. Colecraft

    Methods to engineer ion channels to probe different aspects of channel structure-function and regulation.

    1: Selection and validation of orthogonal tRNA / synthetase pairs for the encoding of unnatural amino acids across kingdoms. Christopher Ahern

    2: Ion channel engineering using protein trans-splicing. Stephan Alexander Pless

    3: ANAP: a versatile, fluorescent probe of ion channel gating and regulation. Michael C. Puljung

    4: Engineering voltage sensing phosphatase, VSP. Yasushi Okamura

    5: Probing Ion Channel Neighborhoods Using APEX. Steven Marx

    Methods to control and characterize ion channel function

    6: Controlling ion channel trafficking by targeted ubiquination and deubiquitination. Henry M. Colecraft

    7: Production of recombinant venom peptides as tools for ion channel research. Eitan Reuveny

    8: Tethered Peptide Toxins for Ion Channels. Steven A. N. Goldstein

    9: Stretch and poke stimulation for characterizing mechanically activated ion channels. Jörg Grandl

    10: Optogenetic control of calcium influx in mammalian cells. YUBIN ZHOU

    11: A method for assessing ionic and molecular permeation in connexin hemichannels. JORGE E. CONTRERAS

    Methods to characterize ion channel function and expression in physiology and disease

    12: Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function. Polina V. Lishko

    13: Live-cell measurements of individual melanosome pH using the novel ratiometric indicator RpHiMEL. Elena Oancea

    14: Long Read Transcript Profiling of Ion Channel Splice Isoforms. Liz Tunbridge

    15: Evaluating BEST1 Mutations in Pluripotent Stem Cell-derived Retinal Pigment Epithelial Cells. Tingting Yang

    16: High Throughput Electrophysiological Evaluation of Mutant Ion Channels. Alfred L. George Jr.

    17: In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy

    Nenad Bursac

Product details

  • No. of pages: 446
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: June 12, 2021
  • Imprint: Academic Press
  • eBook ISBN: 9780128239254
  • Hardcover ISBN: 9780128239247

About the Serial Volume Editors

Daniel Minor

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA

Affiliations and Expertise

Professor, University of California San Francisco, CA, USA

Henry Colecraft

Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Affiliations and Expertise

Professor of Physiology and Cellular Biophysics and Cellular Biophysics, Columbia University College of Physicians and Surgeons, NY, USA

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