
Ion Channels: Channel Chemical Biology, Engineering, and Physiological Function
Description
Key Features
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
Readership
Table of Contents
Preface
Daniel L. Minor Jr. and Henry M. Colecraft
Methods to engineer ion channels to probe different aspects of channel structure-function and regulation.
1: Selection and validation of orthogonal tRNA / synthetase pairs for the encoding of unnatural amino acids across kingdoms. Christopher Ahern
2: Ion channel engineering using protein trans-splicing. Stephan Alexander Pless
3: ANAP: a versatile, fluorescent probe of ion channel gating and regulation. Michael C. Puljung
4: Engineering voltage sensing phosphatase, VSP. Yasushi Okamura
5: Probing Ion Channel Neighborhoods Using APEX. Steven Marx
Methods to control and characterize ion channel function
6: Controlling ion channel trafficking by targeted ubiquination and deubiquitination. Henry M. Colecraft
7: Production of recombinant venom peptides as tools for ion channel research. Eitan Reuveny
8: Tethered Peptide Toxins for Ion Channels. Steven A. N. Goldstein
9: Stretch and poke stimulation for characterizing mechanically activated ion channels. Jörg Grandl
10: Optogenetic control of calcium influx in mammalian cells. YUBIN ZHOU
11: A method for assessing ionic and molecular permeation in connexin hemichannels. JORGE E. CONTRERAS
Methods to characterize ion channel function and expression in physiology and disease
12: Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function. Polina V. Lishko
13: Live-cell measurements of individual melanosome pH using the novel ratiometric indicator RpHiMEL. Elena Oancea
14: Long Read Transcript Profiling of Ion Channel Splice Isoforms. Liz Tunbridge
15: Evaluating BEST1 Mutations in Pluripotent Stem Cell-derived Retinal Pigment Epithelial Cells. Tingting Yang
16: High Throughput Electrophysiological Evaluation of Mutant Ion Channels. Alfred L. George Jr.
17: In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy
Nenad Bursac
Product details
- No. of pages: 446
- Language: English
- Copyright: © Academic Press 2021
- Published: June 12, 2021
- Imprint: Academic Press
- eBook ISBN: 9780128239254
- Hardcover ISBN: 9780128239247
About the Serial Volume Editors
Daniel Minor
Affiliations and Expertise
Henry Colecraft
Affiliations and Expertise
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