This book describes modern electrophysiological techniques for recording and analysis of ion channels in various systems including cells in culture, Xenopus oocytes, brain slices, cellular organelles, and microorganisms. Many chapters deal with the prevention and correction of electrophysiological artifacts. Also included are methods for the purification and expression of ion channel genes and proteins.
@introbul:Key Features @bul:* Applications of electrophysiological techniques
- Expression in heterologous systems
- Reconstitution of lipid bilayers
- Purification and characterization of ion channel proteins and genes
- Recording of ion channels of intracellular membranes and microorganisms
- Data storage and analysis
Biochemists, cell biologists, molecular biologists, physiologists, geneticists, biophysicists, neurophysiologists, microbiologists, and pharmacologists.
Contributors. Preface. Volumes in Series.
Section I: Modern Applications of Electrophysiological Techniques: Patch Clamp Techniques: An Overview, M. Cahalan and E. Neher. Constructing a Patch Clamp Setup, R.A. Levis and J.L. Rae. Glass Technology for Patch Clamp Electrodes, J.L. Rae and R.A. Levis. Ion Channel Selectivity, Permeation, and Block, T. Begenisich. Access Resistance and Space Clamp Problems Associated with Whole-Cell Patch Clamping, C.M. Armstrong and W.F. Gilly. Correction for Liquid Junction Potentials in Patch Clamp Experiments, E. Neher. Nonstationary Noise Analysis and Application to Patch Clamp Recordings, S.H. Heinemann and F. Conti. Prevention of Rundown in Electrophysiological Recording, R. Horn and S.J. Korn. Patch Voltage Clamping with Low-Resistance Seals: Loose Patch Clamp, W.M. Roberts and W. Almers. Perfusing Patch Pipettes, J.M. Tang, J. Wang, and R.S. Eisenberg. Whole-Cell Recording of Calcium Channel Currents, B.P. Bean. Recording from Calcium-Activated Potassium Channels, B.S. Pallotta, A.L. Blatz and K.L. Magleby. Patch-Clamping Cells in Sliced Tissue Preparations, F.A. Edwards and A. Konnerth.
Section II. Expression of Ion Channels: A.: Expression of Ion Channels in Xenopus Oocytes:
Xenopus Oocyte Microinjection: From Gene to Protein, H. Soreq and S. Seidman. Maintenance of Xenopus laevis and Oocyte Injection, A.L. Goldin. Preparation of RNA for Injection into Xenopus Oocytes, A.L. Goldin and K. Sumikawa. Tissue RNA as Source of Ion Channels and Recept
- No. of pages:
- © Academic Press 1992
- 27th July 1992
- Academic Press
- eBook ISBN:
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY