Ion Channels, Volume 207

1st Edition

Print ISBN: 9780121821081
eBook ISBN: 9780080925493
Imprint: Academic Press
Published Date: 27th July 1992
Page Count: 917


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This book describes modern electrophysiological techniques for recording and analysis of ion channels in various systems including cells in culture, Xenopus oocytes, brain slices, cellular organelles, and microorganisms. Many chapters deal with the prevention and correction of electrophysiological artifacts. Also included are methods for the purification and expression of ion channel genes and proteins.

Key Features

@introbul:Key Features @bul:* Applications of electrophysiological techniques

  • Expression in heterologous systems
  • Reconstitution of lipid bilayers
  • Purification and characterization of ion channel proteins and genes
  • Recording of ion channels of intracellular membranes and microorganisms
  • Data storage and analysis


Biochemists, cell biologists, molecular biologists, physiologists, geneticists, biophysicists, neurophysiologists, microbiologists, and pharmacologists.

Table of Contents

Contributors. Preface. Volumes in Series.

Section I: Modern Applications of Electrophysiological Techniques: Patch Clamp Techniques: An Overview, M. Cahalan and E. Neher. Constructing a Patch Clamp Setup, R.A. Levis and J.L. Rae. Glass Technology for Patch Clamp Electrodes, J.L. Rae and R.A. Levis. Ion Channel Selectivity, Permeation, and Block, T. Begenisich. Access Resistance and Space Clamp Problems Associated with Whole-Cell Patch Clamping, C.M. Armstrong and W.F. Gilly. Correction for Liquid Junction Potentials in Patch Clamp Experiments, E. Neher. Nonstationary Noise Analysis and Application to Patch Clamp Recordings, S.H. Heinemann and F. Conti. Prevention of Rundown in Electrophysiological Recording, R. Horn and S.J. Korn. Patch Voltage Clamping with Low-Resistance Seals: Loose Patch Clamp, W.M. Roberts and W. Almers. Perfusing Patch Pipettes, J.M. Tang, J. Wang, and R.S. Eisenberg. Whole-Cell Recording of Calcium Channel Currents, B.P. Bean. Recording from Calcium-Activated Potassium Channels, B.S. Pallotta, A.L. Blatz and K.L. Magleby. Patch-Clamping Cells in Sliced Tissue Preparations, F.A. Edwards and A. Konnerth.

Section II. Expression of Ion Channels: A.: Expression of Ion Channels in Xenopus Oocytes:

Xenopus Oocyte Microinjection: From Gene to Protein, H. Soreq and S. Seidman. Maintenance of Xenopus laevis and Oocyte Injection, A.L. Goldin. Preparation of RNA for Injection into Xenopus Oocytes, A.L. Goldin and K. Sumikawa. Tissue RNA as Source of Ion Channels and Recept


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