Introductory Experiments on Biomolecules and their Interactions - 1st Edition - ISBN: 9780128009697, 9780128010129

Introductory Experiments on Biomolecules and their Interactions

1st Edition

Authors: Robert Delong Qiongqiong Zhou
eBook ISBN: 9780128010129
Paperback ISBN: 9780128009697
Imprint: Academic Press
Published Date: 27th March 2015
Page Count: 126
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Description

Introductory Experiments on Biomolecules and their Interactions provides a novel approach to teaching biomolecules in the lab. While featuring the requisite fundamentals, it also captures the author’s experience in industry, thus providing unique, up-to-date experiments which take the learning experience one-step further.

The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class.

Key Features

  • Features quantitative analysis of RNA degradation by RNase
  • Contains problem sets, calculations, and references for each lab fully immersing students in the learning process
  • Includes instruction on how to maintain a lab notebook and write a formal lab report
  • Provides hands-on engagement with the four major types of biomolecules and “real-life and better applied” examples of molecular interactions

Readership

Undergraduate and graduate students studying biology, biochemistry, chemistry and biotechnology

Table of Contents

  • Introduction
  • About the Authors
  • Master Materials List
  • Format of the Lab Notebook and Formal Lab Report
  • Lab Safety and Policies
  • Experiment 1. An Introduction to Basic Math and Operations in the Biomolecular Laboratory
    • Safety and Hazards
    • Introduction
    • Technical Review
    • Equipment Highlight
    • Experiment 1
    • Methods and Procedure
    • Results and Discussion
    • Problem Set
  • Experiment 2. Preparing Buffers at a Specific Molarity and pH
    • Safety and Hazards
    • Introduction
    • Technical Review
    • Equipment Highlight
    • Experiment 2
    • Results and Discussion
    • Problem Set
  • Experiment 3. Investigating the Physico-Chemical Properties of Amino Acids and Their Analysis by Thin Layer Chromatography
    • Safety and Hazards
    • Introduction
    • Technical Review
    • Equipment Highlight
    • Experiment 3
    • Methods and Procedure
    • Results and Observations
    • Discussion
    • Problem Set
  • Experiment 4. Rapid Purification, Gel Electrophoresis, and Enzyme Activity Assay of the Luciferase Enzyme from Fireflies
    • Safety and Hazards
    • Introduction
    • Technical Review and Equipment Highlights
    • Experiment 4
    • Results and Discussion
    • Problem Set
  • Experiment 5. Hexokinase and G6PDH Catalyzed Reactions of Glucose Measurement
    • Safety and Hazards
    • Introduction
    • Technical Review
    • Equipment Highlight
    • Experiment 5
    • Results and Discussion
    • Problem Set
  • Experiment 6. Polymerase Chain Reaction (PCR)
    • Safety and Hazards
    • Introduction
    • Technical Review
    • Experiment 6
    • Results and Discussion
    • Problem Set
  • Experiment 7. Investigating Protein:Nucleic Acid Interactions by Electrophoretic Mobility Shift Assay (EMSA)
    • Safety and Hazards
    • Introduction
    • Equipment Highlights
    • Experiment 7
    • Results and Discussion
    • Problem Set
  • Experiment 8. Qualitative Analysis of the Degradation of RNA via Ribonuclease A versus B
    • Safety and Hazards
    • Introduction
    • Experiment 8
    • Results and Discussion
    • Problem Set
  • Experiment 9. Preparation of a Fluorescently Labeled Liposome and Its Analysis by Fluorescence Microscopy
    • Safety and Hazards
    • Introduction
    • Experiment 9
    • Results and Discussion
    • Problem Set
  • Experiment 10. Studying Cell-like Structures with Liposome, DNA, and Protein
    • Safety and Hazards
    • Introduction
    • Experiment 10
    • Methods and Procedures
    • Results and Discussion
    • Additional Experiment
  • Index

Details

No. of pages:
126
Language:
English
Copyright:
© Academic Press 2016
Published:
Imprint:
Academic Press
eBook ISBN:
9780128010129
Paperback ISBN:
9780128009697

About the Author

Robert Delong

Affiliations and Expertise

Nanotechnology Innovation Center, Kansas State University, Manhattan, KS, USA

Qiongqiong Zhou

Dr. Qiongqiong Zhou is an assistant professor at Missouri State University, where she studies cytoskeleton dynamics in her lab and teaches several core courses related to biomedical sciences. Prior to that, she was a research fellow in Douglas Robinson’s lab at Johns Hopkins University and Ulrike Eggert’s lab at Harvard Medical School.

Dr. Zhou graduated with a major of life sciences in 2002 from Fudan University in Shanghai, China. Then, she earned her Doctoral degree from University of Southern California, where she studies neurodegerative diseases with Dr. Enrique Cadenas.

With a passion for undergraduate education, Dr. Zhou has led the lab of Biomolecular Interactions for more than 6 semesters using and continuously improving this lab manual. She has the most direct laboratory experience of these experiments with students.

Affiliations and Expertise

Biomedical Sciences, Missouri State University, Springfield, MO, USA