Introduction to Electron Microscopy, Second Edition provides an introduction to the foundations of electron microscopy; an outline of some practical aspects of instrument operation; and discussion of the rationale of the methodology of biological specimen preparation. The book seeks to provide a comprehensive understanding of the theoretical and operational aspects of the electron microscope. This edition consists of two parts. Part One deals with the history, basic theory, and operation of the electron microscope. Part Two discusses steps used in material preparation for electron microscope investigation such as fixation, embedding, and staining techniques. Biomedical researchers, molecular biologists, toxicologists, forensic investigators, and medical students will find this book a very useful reference.
Part One: Instrumentation1. Introduction 2. Historical Review 3. Basic Theory of Electron Microscopy A. Nature of Light Beams B. Resolution C. Nature of Electron Beams D. Electron Emission E. Electron Optics 4. The Electron Microscope A. Illumination System B. Imaging System C. Image Translating System D. Other Electron Microscope Components E. Operational Requirements F. Operation of the Electron Microscope G. Accessories for the Electron Microscope H. Other Types of Electron Microscopes I. Differences between the Light and Electron Microscopes
Part Two: Methodology5. Introduction 6. Historical Review 7. Obtaining of the Specimen A. Preparation of Excised Specimens B. In Situ Fixation C. Perfusion D. Handling of Small Specimens E. Handling of Human Material F. Discussion 8. Fixation A. Osmium Tetroxide Fixation B. Other Fixation Media C. Discussion 9. Dehydration A. Dehydration Media B. Duration of Dehydration 10. Embedding A. Methacrylate B. Water-Soluble Embedding Media C. Polyester Resins D. Epoxy Resins E. Discussion 11. Microtomy A. Principles of Microtomy B. Ultramicrotomes C. Knives D. The Trough E. Section Thickness F. Discussion 12. Staining A. Principles of Staining B. Positive Staining C. Negative Staining 13. Specialized Electron Microscope Techniques A. Ultrastructural Nucleoprotein Localization B. Ultrastructural Enzyme Cytochemistry C. Tracers in Electron Microscopy D. Ultrastructural Immuno-
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- © Pergamon 1970
- 1st January 1970
- eBook ISBN:
Former Professor of Anatomy, Long Island University, Brooklyn; Former Professor and Head, Department of Anatomy, New York Institute of Technology, Old Westbury, NY