Intrinsically Disordered Proteins - 1st Edition - ISBN: 9780128156490, 9780128156506

Intrinsically Disordered Proteins, Volume 611

1st Edition

Serial Volume Editors: Elizabeth Rhoades
eBook ISBN: 9780128156506
Hardcover ISBN: 9780128156490
Imprint: Academic Press
Published Date: 26th November 2018
Page Count: 754
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Table of Contents

    1. Phase Separation of Intrinsically Disordered Proteins
    2. Ammon E. Posey, Alex S. Holehouse and Rohit V. Pappu

    3. Methods and Strategies to Quantify Phase Separation of Disordered Proteins
    4. Alfredo Vidal Ceballos, Charles J. McDonald and Shana Elbaum-Garfinkle

    5. Visualization and Quantitation of Phase-Separated Droplet Formation by Human HP1α
    6. Madeline M. Keenen, Adam G. Larson and Geeta J. Narlikar

    7. Probing RNA Structure in Liquid–Liquid Phase Separation Using SHAPE-MaP
    8. Erin M. Langdon and Amy S. Gladfelter

    9. The Use of 13C Direct-Detect NMR to Characterize Flexible and Disordered Proteins
    10. Erik C. Cook, Grace A. Usher and Scott A. Showalter

    11. Using NMR Chemical Shifts to Determine Residue-Specific Secondary Structure Populations for Intrinsically Disordered Proteins
    12. Wade M. Borcherds and Gary W. Daughdrill

    13. Experimental Characterization of Fuzzy Protein Assemblies: Interactions of Paramyxoviral NTAIL Domains With Their Functional Partners
    14. Francesca Troilo, Christophe Bignon, Stefano Gianni, Monika Fuxreiter and Sonia Longhi

    15. Characterization of Dynamic IDP Complexes by NMR Spectroscopy
    16. Andreas Prestel, Katrine Bugge, Lasse Staby, Ruth Hendus-Altenburger and Birthe B. Kragelund

    17. Spectroscopic Characterization of Structure–Function Relationships in the Intrinsically Disordered Protein Complexin
    18. David Snead and David Eliezer

    19. Distances, Distance Distributions, and Ensembles of Unfolded and Intrinsically Disordered Proteins From Single-Molecule FRET
    20. Erik D. Holmstrom, Andrea Holla, Wenwei Zheng, Daniel Nettles, Robert B. Best and Benjamin Schuler

    21. Probing Differential Binding Mechanisms of Phenylalanine-Glycine-Rich Nucleoporins by Single-Molecule FRET
    22. Piau Siong Tan and Edward A. Lemke

    23. Fluorescence Depolarization Kinetics to Study the Conformational Preference, Structural Plasticity, Binding, and Assembly of Intrinsically Disordered Proteins
    24. Anupa Majumdar and Samrat Mukhopadhyay

    25. Building, Characterization, and Applications of Cuvette-FCS in Denaturant-Induced Expansion of Globular and Disordered Proteins
    26. Timir Baran Sil, Bankanidhi Sahoo and Kanchan Garai

    27. Stopped-Flow Kinetic Techniques for Studying Binding Reactions of Intrinsically Disordered Proteins
    28. Michael D. Crabtree and Sarah L. Shammas

    29. The Use of Mass Spectrometry to Examine IDPs: Unique Insights and Caveats
    30. Dale Stuchfield, Aidan France, Lukasz G. Migas, Anja Thalhammer, Anne Bremer, Bruno Bellina and Perdita E. Barran

    31. Characterizing Time-of-Day Conformational Changes in the Intrinsically Disordered Proteins of the Circadian Clock
    32. Jacqueline F. Pelham, Alexander E. Mosier and Jennifer M. Hurley

    33. Assessing Allostery in Intrinsically Disordered Proteins With Ensemble Allosteric Model
    34. Jing Li and Vincent J. Hilser

    35. A Tethered Vesicle Assay for High-Throughput Quantification of Membrane Fission
    36. Wilton T. Snead and Jeanne C. Stachowiak

    37. Generating Novel Materials Using the Intrinsically Disordered Protein Ubx
    38. Gabriela Geraldo Mendes, Rebecca M. Booth, Donna L. Pattison, Adrian J. Alvarez and Sarah E. Bondos

    39. Challenges in the Structural–Functional Characterization of Multidomain, Partially Disordered Proteins CBP and p300: Preparing Native Proteins and Developing Nanobody Tools
    40. Angela Bekesi, Sara Abdellaoui, Natalie Holroyd, Wouter Van Delm, Els Pardon, Jarne Pauwels, Kris Gevaert, Jan Steyaert, Stefaan Derveaux, Antoni Borysik and Peter Tompa

    41. Characterization of the Binding of Small Molecules to Intrinsically Disordered Proteins
    42. Veselin S. Dobrev, Lisette M. Fred, Kaitlyn P. Gerhart and Steven J. Metallo

    43. Targeting the Intrinsically Disordered Proteome Using Small Molecule Ligands

Sławomir Wójcik, Melissa Birol, Elizabeth Rhoades, Andrew D. Miranker and Zachary A. Levine


Description

Intrinsically Disordered Proteins, Volume 611, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics of interest, including the Characterization of Structure-Function relationships in the intrinsically disordered protein complexin, Distances, distance distributions, and ensembles of IDPs from single-molecule FRET, Biophysical characterization of disordered protein liquid phases, The Use of Mass Spectrometry to Examine IDPs – Unique Insights and Caveats, Fluorescence Depolarization Kinetics to Study Conformational Preference, Structural Plasticity and Membrane Binding of Intrinsically Disordered Proteins, Characterizing the Function of Intrinsically Disordered Proteins in the Circadian Clock, and more.

Key Features

  • Breadth of experimental approaches and systems that will be covered
  • The expertise of the contributors writing the articles

Readership

Basic research scientists – biophysicists, biochemists, and biologists – who are studying systems where protein disorder is important to function or dysfunction.


Details

No. of pages:
754
Language:
English
Copyright:
© Academic Press 2018
Published:
Imprint:
Academic Press
eBook ISBN:
9780128156506
Hardcover ISBN:
9780128156490

Ratings and Reviews


About the Serial Volume Editors

Elizabeth Rhoades Serial Volume Editor

Elizabeth Rhoades was born and raised in Huntsville, Alabama. As a child, she loved reading and when she left for college, she intended to major in English at Duke University. After taking a Physics course to fulfill a distribution requirement during her first year, she switched gears and decided to pursue a Physics major. In between her junior and senior years, she spent a summer at the University of Tennessee in a REU program, where she discovered biophysics. Liz earned her Ph.D. in 2001 from the Biophysics Research Division at the University of Michigan, working with Ari Gafni, a biophysical chemist, and Ducan Steel, a physicist, on understanding amyloid aggregation. It was in the Gafni-Steel lab that she became interested in understanding the physico-chemical basis of protein structure and dynamics and the power of fluorescence spectroscopy as a tool. She spent 2.5 years as a postdoctoral fellow with Gilad Haran at the Weizmann Institute, applying single molecule fluorescence to the study of protein folding, and then an additional 2.5 years as a postdoc in Watt Webb’s lab at Cornell University. In 2006, Liz joined the faculty in the Molecular Biophysics & Biochemistry Department at Yale University, where she rose through the academic ranks. She moved to the Chemistry Department at the University of Pennsylvania in 2016. In her independent career, her research focuses on understanding both functional and dysfunctional aspects of intrinsically disordered proteins, with a focus on several implicated in neurodegeneration. She has served as chair of the Intrinsically Disordered Protein Subgroup of the Biophysical Society and currently serves as Associate Editor of the Proteins section of the Biophysical Journal. Her husband is also a scientist/professor and she has two young sons who plan to be superheroes when they grow up.

Affiliations and Expertise

Associate Professor, Department of Chemistry, University of Pennsylvania, Philadelphia, PA, USA