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Interleukin 3 - 1st Edition - ISBN: 9780124320154, 9781483268163

Interleukin 3

1st Edition

The Panspecific Hemopoietin

Editor: John W. Schrader
eBook ISBN: 9781483268163
Imprint: Academic Press
Published Date: 28th February 1988
Page Count: 444
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Interleukin 3: The Panspecific Hemopoietin contains contributions from immunologists, hematologists, protein chemists, and molecular biologists whose efforts have defined the biology and molecular nature of interleukin 3 (IL-3) in less than a decade. The articles represent personal perspectives rather than exhaustive reviews. They are arranged in alphabetical order rather than thematically because many of the individual articles cover multiple aspects of the molecule. These articles cover topics such as the potential role of IL-3 in the pathology of allergic and inflammatory disease and the role of IL-3 in the genesis of certain myeloid leukemias. A number of articles also highlight an important technical point, namely, that many of the various assays often used for IL-3 are not specific. One article indicates that the ""histamine-producing cell-stimulating factor"" activity can be mediated not only by IL-3 but also by granulocyte-macrophage colony-stimulating factor. Another deals with the lack of specificity of assays based on the induction of 20α-SDH or factor-dependent cell lines.

Table of Contents


Molecular Structure and Biological Activities of P Cell-Stimulating Factor (Interieukin 3)

I. Introduction

II. History and Nomenclature

III. Cellular Sources and Assays

IV. Biological Activities

V. Characterization, Purification, and Structure

VI. Structure-Function Studies Approached by Peptide Synthesis

VII. Summary


Histamine-Producing Cell-Stimulating Factor (HCSF) and Interieukin 3 (IL-3): Their Effects on Histidine and Ornithine Decarboxylases

I. Introduction

II. Evidence for a Lymphokine Increasing Histamine Synthesis: Histamine-Producing Cell-Stimulating Factor

III. Interieukin 3 (IL-3) and Histamine-Producing Cell-Stimulating Factor

IV. Interieukin 3 and Arginine Metabolism in Hemopoietic Cells

V. Summary

VI. Addendum


IL-3 and the Development of Cultured (Mucosal) Mast Cells in the Rat

I. Introduction

II. Mast Cells In Vivo and In Vitro

III. IL-3-Like Activity in the Rat

IV. The Target Cells Responding to IL-3

V. Maturation of IL-3-Dependent Cell Types

VI. Conclusions and Speculations: IL-3 and MMC in Disease

VII. Addendum


Molecular Biology of Interleukin 3: A Multilineage Hemopoietic Growth Regulator

I. Introduction

II. Biological Properties of Interleukin 3

III. Assays for Interleukin 3

IV. Isolation of cDNA Clones for Murine Interleukin 3

V. Cloning of the Gene for Murine Interleukin 3

VI. Expression from Recombinant DNA Constructs

VII. Cloning of the Rat and Human Interleukin 3 Genes

VIII. Interleukin 3 and Leukemia

IX. Monoclonal Antibodies to Interleukin 3

X. Conclusion


Immunological Regulation of Hematopoietic Stem Cell Function by Interleukin 3 and Its Role in Leukemogenesis

I. Introduction

II. Sequence of IL-3-Supported Differentiation In Vitro

III. Relationship of IL-3-Regulated Growth and Differentiation to Leukemogenesis

IV. Mechanisms in IL-3-Regulated Differentiation

V. Mechanisms in IL-3-Regulated Growth

VI. Conclusions and Speculations


The Expression and Characterization of Recombinant Mouse IL-3

I. Introduction

II. Expression of IL-3 in Mammalian Cells

III. Expression of IL-3 in Escherichia Coli

IV. Expression of IL-3 in Yeast

V. Conclusion


The Multipotential Colony-Stimulating Factor, Multi-CSF (IL-3)

I. Terminology

II. Review of Work on Multi-CSF in This Laboratory

III. Biochemical Nature and Molecular Biology of Multi-CSF

IV. Tissue Sources of Multi-CSF

V. Membrane Receptors for Multi-CSF

VI. Biological Actions in Vitro of Multi-CSF

VII. In Vivo Fate of Multi-CSF

VIII. In Vivo Effects of Injected Bacterially Synthesized Multi-CSF

IX. Does Multi-CSF Play a Special Role in Leukemogenesis?

X. Is There a Human Analog of Murine Multi-CSF?

XI. Summary


Interieukin 3: An Overview

I. Introduction

II. Cellular Origins of Interieukin 3

III. Biochemical and Molecular Aspects

IV. Interleukin 3 Receptor Characterization

V. Interleukin 3 in Myeloid Differentiation

VI. Interleukin 3 Action in Erythropoiesis

VII. Interleukin 3 Action in Megakaryocytopoiesis

VIII. Interleukin 3: A Mast Cell and Basophil Growth Factor

IX. Interleukin 3 and Natural Cytotoxic Cells

X. The Role of Interleukin 3 in Β Cell Development

XI. Interleukin 3 Action on Pluripotential Stem Cells

XII. Interleukin 3 and the Proliferation of Factor-Dependent Cell Lines

XIII. In Vivo Action of Interleukin 3

XIV. Interleukin 3 and Murine Myeloid Leukemia—Autocrine Regulation

XV. Conclusion


The Panspecific Hemopoietin Interleukin 3: Physiology and Pathology

I. Introduction

II. Production of IL-3 In Vivo

III. Clearance of IL-3 from the Blood

IV. Action of IL-3 In Vivo

V. Mechanism of Action of IL-3

VI. Pathological Role of IL-3

VII. Role of IL-3 in Neoplasia

VIII. Summary


Mast Cell Subclasses and Their Growth Dependence in Mice, Rats, and Humans

I. Introduction

II. The Mouse Cultured IL-3-Dependent Mast Cell

III. The Rat Cultured Τ Cell Factor-Dependent Mast Cell

IV. Mucosal Mast Cells

V. The Rat Basophilic Leukemic Cell: Mucosal Mast Cell Phenotype

VI. Maintenance of Rat Connective Tissue Mast Cells in Coculture

VII. Cultured Human Τ Cell Factor-Dependent Mast Cells or Basophils

VIII. Human Mast Cell Subclasses In Vivo

IX. Basophils and Eosinophils

X. Summary: The Relationship of Connective Tissue Mast Cells, Mucosal Mast Cells, Basophils, and Eosinophils


Human Growth Factors for Metachromatically Staining Cells

I. Introduction

II. A Human Basophil-Like Cell-Promoting Activity (BaPA)

III. A Growth Factor for Human Mast Cell-Like Cells: IL-3-Like Activity

IV. Biochemical Characteristics of Human IL-3-Like Activity

V. Summary and Perspectives


Note Added in Proof

The Mode of Action of Interieukin 3 in Promoting Survival, Proliferation, and Differentiation of Hemopoietic Progenitor Cells

I. Introduction

II. Long-Term Marrow Cultures and the Development of Interieukin 3-Dependent Cell Lines

III. Purification of Interieukin 3 Using FDC-P Cell Lines

IV. Preparation of Interieukin 3-Dependent Multipotent Stem Cell Lines

V. The Mode of Action of Interieukin 3 in Promoting Survival and Proliferation of Hemopoietic Cells

VI. The Mediation of Transmembrane Signaling Events by Interleukin 3

VII. Intracellular Mechanisms Governing Stem Cell Differentiation

VIII. Possible Roles of Interleukin 3 in Long-Term Marrow Cultures and In Vivo


Molecular Cloning of a Primate cDNA and the Human Gene for Interleukin 3

I. Introduction

II. Molecular Cloning and Expression of the Primate IL-3 cDNA

III. The Human IL-3 Gene

IV. Conclusions


Isolation and Characterization of the Mouse Interleukin 3 Gene and Its Expression in Activated T Cells

I. Introduction

II. Isolation of cDNA Clones Encoding Interleukin 3

III. Structure of the IL-3 Gene

IV. Expression of IL-3 mRNA

V. Constitutive Expression of the IL-3 Gene

VI. Transcription of the IL-3 Gene In Vitro




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© Academic Press 1988
28th February 1988
Academic Press
eBook ISBN:

About the Editor

John W. Schrader

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