Interleukin 2 - 1st Edition - ISBN: 9780126514209, 9780323139946

Interleukin 2

1st Edition

Authors: Kendall A Smith
eBook ISBN: 9780323139946
Imprint: Academic Press
Published Date: 28th September 1988
Page Count: 264
Sales tax will be calculated at check-out Price includes VAT/GST
15% off
15% off
15% off
Price includes VAT/GST
× DRM-Free

Easy - Download and start reading immediately. There’s no activation process to access eBooks; all eBooks are fully searchable, and enabled for copying, pasting, and printing.

Flexible - Read on multiple operating systems and devices. Easily read eBooks on smart phones, computers, or any eBook readers, including Kindle.

Open - Buy once, receive and download all available eBook formats, including PDF, EPUB, and Mobi (for Kindle).

Institutional Access

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.


Interleukin 2 focuses on interleukin 2 (IL-2) and its impact on immunology. Topics covered include IL-2's mechanism of action, the structure of the IL-2 receptor, the characteristics of natural and recombinant human IL-2, regulation of IL-2 production by the T cell antigen receptor, how IL-2 binding induces the transcription of a novel set of genes, and the pathological roles of IL-2 receptor inducers in adult T cell leukemia. This book is comprised of 12 chapters and begins with a historical overview of IL-2, from the discovery of soluble mitogenic factors in culture media of stimulated lymphocytes to the discovery of IL-2 as the molecule responsible for stimulating T cell proliferation. The physicochemical and biological properties of purified recombinant IL-2 are then described, paying particular attention to natural IL-2 derived from human peripheral blood leukocytes. The chapters that follow discuss the different techniques used to gain insight into the functionally important regions of IL-2, the role of the T cell antigen receptor in regulating the production of IL-2, and the structure of the human high-affinity IL-2 receptor. The book also describes transmembrane signaling by IL-2, IL-2-inducible gene expression in T lymphocytes, abnormal IL-2 receptor expression in adult T cell leukemia, the use of IL-2 receptors in immunosuppressive therapy, and the use of IL-2 as a pharmacologic reagent. This book will be of interest to students and researchers in immunology and biochemistry.

Table of Contents




1. Interleukin 2: A 10-Year Perspective

I. Introduction

II. Interleukin 2

III. The IL-2 Receptor

IV. IL-2 Receptor Structure

V. Functional Consequences of the IL-2 Receptor Bimolecular Structure

VI. α Chains Are Responsible for Stimulating Cell Cycle Progression

VII. The Regulation of IL-2 Receptor Expression

VIII. The IL-2 Receptor Regulation of T Cell Growth

IX. IL-2 Stimulates T Cell G1 Progression and c-myb Expression

X. Conclusions


2. Characteristics of Natural and Recombinant Human Interleukin 2

I. Introduction

II. Natural IL-2 Derived from Human PBL

III. Recombinant IL-2 Derived from E. coli

IV. Comparison of Biological Activities for Natural and Recombinant IL-2

V. Summary and Conclusions


3. Interleukin 2: Approaches to Structure Determination

I. Introduction

II. Preliminary Data

III. Species Homology

IV. Antibodies as Structure Probes

V. Recombinant DNA-Derived Proteins and Site-Directed Mutagenesis

VI. Peptide Synthesis

VII. Molecular Modeling and Graphics

VIII. Conclusion


4. Regulation of Interleukin 2 Production by the T Cell Antigen Receptor

I. Introduction

II. The T3/Ti Complex: Obligate Coexpression of T3 with Ti

III. Jurkat as a Model for the Study of T3/Ti-Mediated Activation

IV. The Role of Increases in the Concentration of Cytoplasmic Free Ca2+ in T3/Ti-Mediated Signaling

V. T3/Ti-Mediated Turnover of Polyphosphoinositides

VI. T3/Ti Activates Protein Kinase C

VII. Cholera Toxin Inhibits T3/Ti-Mediated Polyphosphoinositide Turnover

VIII. Induction of IL-2 Transcripts Requires Sustained T3/Ti-Ligand Interactions

IX. Summary and Future Directions


5. New Perspectives on the Structure of the Human High-Affinity Interleukin 2 Receptor

I. Introduction

II. Characterization of the Human IL-2 Receptor (Tac Antigen)

III. High- and Low-Affinity Forms of the IL-2 Receptor

IV. Expression of the Tac cDNA in Eukaryotic Cells

V. Structural Analysis of the High-Affinity IL-2 Receptor


6. Transmembrane Signaling by Interleukin 2

I. Introduction

II. Transmembrane Events in IL-2-Induced Proliferation

III. Delayed Events

IV. Oncogene Expression

V. Discussion


7. Mechanisms of Interleukin 2 Action: IL-2-Inducible Gene Expression in T Lymphocytes

I. Introduction

II. Early Molecular Events Associated with the Interaction of IL-2 and IL-2 Receptor: Potential Involvement of Protein Kinase C and Calcium

III. Rationale for Investigating the Expression of Protooncogenes and Other Genes in IL-2-Stimulated T Lymphocytes

IV. IL-2-Inducible Expression of Genes in Activated T Lymphocytes

V. Some Possible Explanations for the Patterns of Gene Expression Induced by IL-2, Phorbol Esters, and Mitogenic Lectins in Activated T Lymphocytes

VI. Mechanisms of IL-2-Mediated Gene Expression

VII. IL-2-Mediated Signal Transduction: Speculations and Future Directions


8. Interleukin 2 Binding Induces Transcription of a Novel Set of Genes: Implications for T Lymphocyte Population Dynamics

I. Introduction

II. Modes of IL-2 Induction

III. Mechanics of IL-2 Action

IV. Measures of IL-2 Responsiveness

V Significance of Positive IL-2 Receptor Regulation

VI. Conclusion


9. Abnormal Interleukin 2 Receptor Expression in Adult T Cell Leukemia

I. Introduction

II. Development of Anti-Tac Monoclonal Antibody

III. Identification of Anti-Tac Antibody as an Anti-IL-2 Receptor Antibody

IV. Characterization of IL-2 Receptor

V. IL-2R Expression in B Cells

VI. IL-2R Expression in ATL

VII. HTLV-I Infection and IL-2R Expression

VIII. Possible Role of IL-2 Receptor in the Leukemogenesis of ATL

IX. IL-2R Expression in T Cell Chronic Lymphocytic Leukemia

X. Summary


10. Interleukin 2 Receptor Inducers: Pathological Roles in Adult T Cell Leukemia

I. Introduction

II. Studies of IL-2R and ATL

III. Properties of Human IL-2R Protein

IV. Cloning of IL-2R Gene

V. Humoral Control of IL-2R Expression

VI. Abnormal Regulation of IL-2R Expression in ATL

VII. ADF Inducing IL-2R Expression

VIII. IL-2-Dependent and -Independent ATL: Stepwise Progression Model

IX. Possible Roles of IL-2R Dysregulation in ATL

X. Conclusion


11. Interleukin 2 Receptor-Directed Immunosuppressive Therapy

I. Introduction

II. Effect of M7/20 Treatment on Murine Cardiac Allografts

III. Effect of M7/20 Treatment of Murine Skin Allografts

IV. Effect of M7/20 and Anti-L3T4 Antibodies on Delayed-Type Hypersensitivity (DTH): The Role of Complement and Epitope

V. Effect of M7/20 Anti-IL-2R Antibody on Murine Autoimmunity: Diabetes Mellitus and Systemic Lupus Erythematosus Models

VI. Effect of ART18 Anti-IL-2R Antibody Treatment on Rat Cardiac Allografts

VII. Synergistic Effect of Anti-IL-2R mAb and Cyclosporin (CsA) Treatment

VIII. Effect of Anti-IL-2R on Graft Infiltration

IX. Effect of ART18 Treatment on Host Alloreactivity

X. Effect of ART18 Therapy on Pancreatic Islet Allografts

XI. Anti-IL-2R mAb Treatment of Subhuman and Human Primate Recipients of Kidney Allografts

XII. Development of a Recombinant IL-2 Toxin by Gene Fusion Technique

XIII. Discussion


12. Interleukin 2 as a Pharmacologic Reagent

I. Introduction

II. Rationale for the Use of IL-2 In Vivo

III. Immunotherapeutic Effects of IL-2 in Murine Models

IV. Human Studies of IL-2 Administration

V. Summary and Conclusions




No. of pages:
© Academic Press 1988
Academic Press
eBook ISBN:

About the Author

Kendall A Smith

Ratings and Reviews