Interacting Macromolecules

Interacting Macromolecules

The Theory and Practice of Their Electrophoresis, Ultracentrifugation, and Chromatography

1st Edition - January 1, 1970

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  • Author: John Cann
  • eBook ISBN: 9780323158657

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Interacting Macromolecules: The Theory and Practice of Their Electrophoresis, Ultracentrifugation, and Chromatography reviews advances in theory and practice concerning the electrophoresis, ultracentrifugation, and chromatography of interacting macromolecules. The principles of mass transport of non-interacting systems are discussed, along with the weak electrolyte moving-boundary theory and analytical solution of approximate transport equations for certain types of interactions. Computer computations on ligand-mediated association-dissociation reactions are also presented. This book is comprised of six chapters and begins with a survey of the principles of electrophoresis and ultracentrifugation of non-reacting systems before proceeding with a detailed treatment of the mass transport of reversibly reacting macromolecules. A conservation equation is derived for a solution containing a single macromolecular ion. The following chapters explore the weak-electrolyte moving-boundary theory; the analytical Solution of approximate conservation equations; and numerical solution of exact conservation equations. The formulation of the numerical computation for ligand-mediated association-dissociation reactions is described, together with a code for sedimentation calculations. The final chapter summarizes the procedures and precautions required to assure accurate interpretation of sedimentation and electrophoretic patterns in terms of the thermodynamic and molecular parameters characterizing the reactions exhibited by biological macromolecules. The more common analytical applications of ultracentrifugation, electrophoresis, and chromatography are also outlined. This monograph is intended for molecular biologists and graduate students.

Table of Contents

  • Preface


    I. Principles of Electrophoresis and Ultracentrifugation



    Appendix I: Some Other Methods of Sedimentation Analysis

    Appendix II: Total Chemical Potential


    II. Weak-Electrolyte Moving-Boundary Theory

    Macromolecular Isomerization

    Weak-Electrolyte Moving Boundary

    Interactions of Proteins with Small Ions

    Interactions between Different Macromolecules


    III. Analytical Solution of Approximate Conservation Equations

    Analytical Solution of Approximate Conservation Equations

    The Similarity Transformation

    The Gilbert Theory

    The Gilbert-Jenkins Theory


    IV. Numerical Solution of Exact Conservation Equations

    Factors Governing the Precise Shape of Reaction Boundaries

    Zone Transport of Reacting Systems

    Interaction of Macromolecules with Small Molecules


    V. Numerical Methods


    Appendix: A Computer Code for Sedimentation Calculations

    VI. Practical Implications

    Velocity Sedimentation




    Author Index

    Subject Index

Product details

  • No. of pages: 262
  • Language: English
  • Copyright: © Academic Press 1970
  • Published: January 1, 1970
  • Imprint: Academic Press
  • eBook ISBN: 9780323158657

About the Author

John Cann

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