Integrated Methods in Protein Biochemistry: Part C

Integrated Methods in Protein Biochemistry: Part C

1st Edition - January 10, 2023

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  • Editor: Arun Shukla
  • Hardcover ISBN: 9780443185922

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Description

Integrated Methods in Protein Biochemistry: Part C, Volume 679 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling, Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems, Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more. Other sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Functional characterization of lytic polysaccharide monooxygenases (LPMOs), Characterization of RRE domain in RiPP biosynthesis, The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations, Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging, and more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in Methods in Enzymology serials
  • Updated release includes the latest information on Integrated Methods in Protein Biochemistry

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. NanoBiT-based methods to monitor the activation and modulation of RTKs
    Andy Chevigne
    2. The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling
    Ajith Karunarathne
    3. Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems
    Florian Schubot
    4. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase
    Renwick Dobson
    5. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase
    Thomas C. Brunold and Joshua Miller
    6. DeGlyPHER: MS-based analysis of viral spike N-glycoforms
    John Robert Yates III
    7. Covalent protein painting: MS-based protein footprinting
    John Robert Yates III
    8. Characterization of GPCR signaling complexes using negative-staining electron microscopy
    Arun K. Shukla
    9. Probing protein misfolding and dissociation with free electron laser
    Kazuhiro Nakamura and Hisashi Okumura
    10. Optimized protocol for the characterization of Cas12a activities
    Rakhi Rajan, Lindsie Martin and Saadi Rostami
    11. Proximity proteomics for the identification and characterization of extracellular vesicles
    Norihiro Kotani
    12. Functional characterization of lytic polysaccharide monooxygenases (LPMOs)
    Vincent Eijsink
    13. Characterization of RRE domain in RiPP biosynthesis
    Douglas A. Mitchell
    14. The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations
    Aaron T. Smith
    15. Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging
    Daniel Zicha
    16. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry
    Nobuaki Takemori
    17. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins
    Shawn Chen and Heng Wang
    18. Quantitative cysteinome profiling with DIA-ABPP
    Chu Wang
    19. High-throughput screening and evolution of carbohydrate-active enzymes
    Shishir PS Chundawat
    20. Regulation of enzymes with light using unnatural amino acids
    Andrea Christa Kneuttinger
    21. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies
    Sven Eyckerman
    22. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics
    Emily E. Weinert
    23. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors
    Nicholas Alexander Graham
    24. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules
    Joanna F. McGouran
    25. Reconstitution of the MLL1 complex and probing methyltransferase activity
    Yali Dou
    26. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy
    Geert van den Bogaart
    27. Structural and functional analysis of mono-copper enzymes from fungal species
    28. Structure, function and regulation of deubiquitinases and RNA-modifying proteins.
    Daniel Dempsey
    29. Electrophysiology of ion-transporting rhodopsins
    Hideki Kandori
    30. Measuring protein post-translational modifications using the protein semi-synthesis
    Nam Chu
    31. Assay design and analysis of human Uracil DNA Glycosylase (UNG2)
    Brian Patrick Weiser
    32. Structure and function of T-Cell Protein Tyrosine Phosphatase (TCPTP)
    Tzu-Ching Meng
    33. Gel-based sample preparation methods for top-down/native/cross-linking mass spectrometry
    Nobuaki Takemori
    34. Global substrate profiling of post-translational modifying enzymes
    Charles S. Craik, Anthony O’Donoghue and Conner Bardine
    35. Methods for measuring kinetic isotope effects on the hydroxylation of a peptide
    Michael Knapp

Product details

  • No. of pages: 412
  • Language: English
  • Copyright: © Academic Press 2023
  • Published: January 10, 2023
  • Imprint: Academic Press
  • Hardcover ISBN: 9780443185922

About the Editor

Arun Shukla

Arun Shukla
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Affiliations and Expertise

Indian Institute of Technology, Kanpur, India

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