Integrated Methods in Protein Biochemistry: Part A

Integrated Methods in Protein Biochemistry: Part A

1st Edition - December 1, 2022

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  • Editor: Arun Shukla
  • Hardcover ISBN: 9780323992664

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Description

Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more. Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on Integrated Methods in Protein Biochemistry

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. DNA and protein engineering to create protein bioswitches with new functions
    Stewart Loh
    2. Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases
    Susan Michaelis
    3. An experimental protocol to study lipid transfer proteins
    Haijia Yu
    4. Synthesis of small heat shock proteins
    Matthew Pratt
    5. Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins
    Guang-Yu Yang
    6. An experimental protocol for glycoconjugate analysis
    Yifa Zhou
    7. Methods for proximity-based biotinylation combined with Mass Spectrometry
    Ralph Kehlenbach
    8. Synthetic antibody fragments as conformational sensors of protein activation and trafficking
    Arun K. Shukla
    9. Expression, purification, functional analysis and crystallization of Rag GTPase
    Kuang Shen
    10. Purification of bacterial transcription elongation complexes by photoreversible immobilization
    Eric Strobel
    11. Inhibition of c-Myc-MAX heterodimerization
    Neha Garg
    12. Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases
    Markus C. Wahl
    13. Phosphorylation and subcellular localization of human phospholipase A1
    Atsushi Yamashita
    14. Integrated methods in studying light-sensitive proteins
    Lars-Oliver Essen
    15. Structure and biochemistry of thermophilic protein
    Ankan Banerjee
    16. Using hydrogen/deuterium exchange mass spectrometry to probe protein structure
    Gert Bange
    17. Nanopore force spectroscopy to probe structural dynamics of proteins
    Sonja Schmid
    18. Structural analysis of knotted proteins
    Shang-Te "Danny" Hsu
    19. Mutation-induced structural changes in the SARC-CoV2 spike protein
    Shang-Te "Danny" Hsu
    20. Preparing metabolite capped RNA substrates using RNA polymerases
    Jeremy G. Bird

Product details

  • No. of pages: 412
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: December 1, 2022
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323992664

About the Serial Volume Editor

Arun Shukla

Arun Shukla
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Affiliations and Expertise

Indian Institute of Technology, Kanpur, India

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