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Insect Viruses
Biotechnological Applications
1st Edition, Volume 68 - September 22, 2006
Editor: Byrony C Bonning
Language: English
eBook ISBN:9780080464787
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Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management…Read more
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Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.
Researchers and postgraduates within academia and industry working in virology, entomology, biotechnology, insect pest management and biological control, human gene therapy, vaccinology, plant pathology, drug discovery, and genetics
Preface
Insect Viruses As Laboratory Research Tools
Milestones Leading to the Genetic Engineering of Baculoviruses as Expression Vector Systems and Viral Pesticides
Abstract
I Introduction
II Baculovirus Advances Before the Application of Recombinant DNA
III Recombinant DNA Technologies
IV Quality Improvements: Expression Vectors, Enhanced Expression, Secretion, and Protein Integrity
V Pathways of Baculovirus Invasion and Infection
VI Genetically Engineered Viral Pesticides
VII Other Notable Developments Emerging from Baculovirus Molecular Biology and the BEVS
VIII Conclusions
Acknowledgments
Polydnavirus Genes that Enhance the Baculovirus Expression Vector System
Abstract
I The BEVS: Advantages and Limitations
II Enhancement of the BEVS in Insect Cells: General
III Enhancement of the BEVS in Insect Cells by Campoletis sonorensis Ichnovirus Vankyrin Proteins (Vankyrin-Enhanced BEVS)
Acknowledgments
Baculovirus Display: A Multifunctional Technology for Gene Delivery and Eukaryotic Library Development
Abstract
I Introduction
II Targeting of Baculoviral Vectors by Surface Display
III Altering the Tropism of Baculoviral Vectors Through Pseudotyping
IV Baculovirus Display of Immunogens
V Generation of Display Libraries
VI Summary
Stably Transformed Insect Cell Lines: Tools for Expression of Secreted and Membrane-anchored Proteins and High-throughput Screening Platforms for Drug and Insecticide Discovery
Abstract
I Introduction
II Generation of Stably Transformed Cell Lines
III Expression of Secreted, Intracellular, and Membrane-Anchored Proteins
IV Screening Platforms for Drug and Insecticide Discovery
V Host Cell Engineering
VI Conclusions—Future Perspectives
Acknowledgments
Applications To Human And Animal Health
Protein N-Glycosylation in the Baculovirus–Insect Cell Expression System and Engineering of Insect Cells to Produce “Mammalianized” Recombinant Glycoproteins
Abstract
I Insect Protein N-Glycosylation and Its Importance
II Modification of Lepidopteran N-Glycosylation Pathways for Improved Processing and Function of Glycoproteins Produced with Baculovirus Expression Vectors
III Other Considerations and Future Improvements
IV Concluding Remarks
Acknowledgments
Vaccines for Viral and Parasitic Diseases Produced with Baculovirus Vectors
Abstract
I Introduction to Recombinant Subunit Vaccines
II The Baculovirus–Insect Cell Expression System for Vaccine Production
III Viral Subunits Expressed in the Baculovirus System
IV Baculovirus-Produced Vaccines Against Protozoan Parasites and Helminths
V Conclusions and Prospects
Acknowledgments
Baculoviruses and Mammalian Cell-based Assays for Drug Screening
Publisher Summary
I Introduction to BacMam
II Nuclear Receptors
III Transporters
IV G-Protein–Coupled Receptors
V Ion Channels
VI Viral Targets
VII Conclusions
Acknowledgments
Baculovirus Vectors for Gene Therapy
Abstract
I Baculovirus Transduction of Mammalian Cells
II Baculovirus Vectors for Gene Therapy
III Advantages and Limitations of Baculoviruses as Gene Therapy Vectors
IV Safety Issues Concerning the Use of Baculoviruses for Gene Therapy
V Conclusions and Prospects
Acknowledgments
Insect Pest Management
Genetically Modified Baculoviruses: A Historical Overview and Future Outlook
Abstract
I Introduction
II Biology of Baculoviruses
III Baculoviruses as Insecticides
IV Integration of Ideas, Recombinant Baculoviruses for Pest Control
V A New Era in Recombinant Baculoviruses, Insect-Selective Peptide Toxins
VI Era of Multilateral Development
VII Implementation of a Recombinant Baculovirus Insecticide
VIII Concluding Thoughts
Acknowledgments
Densoviruses for Control and Genetic Manipulation of Mosquitoes
Abstract
I Introduction
II The Biology of Mosquito Densoviruses
III Pathogenesis of Mosquito Densoviruses
IV Densovirus-Transducing Vectors
V Conclusion: Densoviruses and Vector-Borne Disease
Acknowledgments
Potential Uses of Cys-Motif and Other Polydnavirus Genes in Biotechnology
Abstract
I Introduction
II Polydnavirus Life Cycle and Induced Pathologies
III Factors from Parasitoids That Alter Host Physiology
IV Polydnavirus Genes
V CsIV Cys-Motif Genes and Potential Biotechnological Applications
VI Conclusion and Prospects for the Future Use of Polydnavirus Genes
Acknowledgments
Virus-Derived Genes for Insect-Resistant Transgenic Plants
Abstract
I Introduction
II Enzymes That Target the Peritrophic Membrane
III Enzymes That Target the Basement Membrane
IV Delivery of Intrahemocoelic Toxins from Plants
V Concluding Remarks
Acknowledgments
Small RNA Viruses of Insects: Expression in Plants and RNA Silencing
Abstract
I Insect RNA Viruses and Their Use as Biopesticides
II Pathology and Host Biology
III Production of Insect SRVs in Nonhost Systems
IV RNA Silencing in Plants and the Effect on Expression of Transgenes