Novel approaches for the study of phosphodiesterases and phosphoprotein phosphatases, as well as those of nonmammalian cyclic nucleotide receptors, are emphasized for the first time in the Methods of Enzymology series. New methods for the study of protein kinases are also presented.


Biochemists, endocrinologists, cell and molecular biologists, analytical and clinical chemists, biomedical researchers, pharmacologists, physiologists.

Table of Contents

Cyclic Nucleotide Cascades: E. Shacte, E.R. Stadtman, S.R. Jurgensen, and P.B. Chock, Role of cAMP in Cyclic Cascade Regulation. S. Swillens, J.-M. Boeynaems, and J.E. Dumont, Theoretical Considerations of the Regulatory Steps in the cAMP Cascade System. T. Ishikawa, K. Matsumoto, and I. Uno, Yeast Mutants Altered in the cAMP Cascade Systems. Assays of Cyclic Nucleotide Levels, Turnover, and Transport: G. Brooker, Improvements in the Automated Radioimmunoassay for cAMP or cGMP. R. Barber and R.W. Butcher, cAMP turnover in Intact Cells. T.F. Walseth, R.M. Graeff, and N.D. Goldberg, Monitoring Cyclic Nucleotide Metabolism in Intact Cells by 180 Labeling. J.D. Corbin, T.W. Gettys, P.F. Blackmore, S.J. Beebe, S.H. Francis, D.B. Glass, J.B. Redmon, V.S. Sheorain, and L.R. Landiss, Purification and Assay of cAMP, cGMP, AND Cyclic Nucleotide Analogs in Cells Treated with Cyclic Nucleotide Analogs. L.L. Brunton and L.E. Heasley, cAMP Export and Its Regulation by Prostaglandin A1. Cyclic Nucleotide Action: Cyclic Nucleotide Action in Mammals: R. Ekanger and S.O. D~aloskeland, Use of Antibody-Sepharose Columns to Study Hormonal Activation of cAMP-Dependent Protein Kinase Isozymes. S.A. Livesey and T.J. Martin, Selective Activation of the cAMP-Dependent Protein Kinase Isoenzymes. S.J. Beebe, P.F. Blackmore, T.D. Chrisman, and J.D. Corbin, Use of Synergistic Pairs of Site-Selective cAMP Analogs in Intact Cells. M.C. Mumby and C.W. Scott, cAMP-Dependent Protein Kinase Regulatory Subunit Phosphorylation in Intact Cells. S.O. D~aloskeland and D. ~alOgreid, Ammonium Sulfate Precipitation Assay for the Study of Cy


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© 1988
Academic Press
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