Description

Novel approaches for the study of phosphodiesterases and phosphoprotein phosphatases, as well as those of nonmammalian cyclic nucleotide receptors, are emphasized for the first time in the Methods of Enzymology series. New methods for the study of protein kinases are also presented.

Readership

Biochemists, endocrinologists, cell and molecular biologists, analytical and clinical chemists, biomedical researchers, pharmacologists, physiologists.

Table of Contents

Cyclic Nucleotide Cascades: E. Shacte, E.R. Stadtman, S.R. Jurgensen, and P.B. Chock, Role of cAMP in Cyclic Cascade Regulation. S. Swillens, J.-M. Boeynaems, and J.E. Dumont, Theoretical Considerations of the Regulatory Steps in the cAMP Cascade System. T. Ishikawa, K. Matsumoto, and I. Uno, Yeast Mutants Altered in the cAMP Cascade Systems. Assays of Cyclic Nucleotide Levels, Turnover, and Transport: G. Brooker, Improvements in the Automated Radioimmunoassay for cAMP or cGMP. R. Barber and R.W. Butcher, cAMP turnover in Intact Cells. T.F. Walseth, R.M. Graeff, and N.D. Goldberg, Monitoring Cyclic Nucleotide Metabolism in Intact Cells by 180 Labeling. J.D. Corbin, T.W. Gettys, P.F. Blackmore, S.J. Beebe, S.H. Francis, D.B. Glass, J.B. Redmon, V.S. Sheorain, and L.R. Landiss, Purification and Assay of cAMP, cGMP, AND Cyclic Nucleotide Analogs in Cells Treated with Cyclic Nucleotide Analogs. L.L. Brunton and L.E. Heasley, cAMP Export and Its Regulation by Prostaglandin A1. Cyclic Nucleotide Action: Cyclic Nucleotide Action in Mammals: R. Ekanger and S.O. D~aloskeland, Use of Antibody-Sepharose Columns to Study Hormonal Activation of cAMP-Dependent Protein Kinase Isozymes. S.A. Livesey and T.J. Martin, Selective Activation of the cAMP-Dependent Protein Kinase Isoenzymes. S.J. Beebe, P.F. Blackmore, T.D. Chrisman, and J.D. Corbin, Use of Synergistic Pairs of Site-Selective cAMP Analogs in Intact Cells. M.C. Mumby and C.W. Scott, cAMP-Dependent Protein Kinase Regulatory Subunit Phosphorylation in Intact Cells. S.O. D~aloskeland and D. ~alOgreid, Ammonium Sulfate Precipitation Assay for the Study of Cy

Details

No. of pages:
850
Language:
English
Copyright:
© 1988
Published:
Imprint:
Academic Press
eBook ISBN:
9780080882765
Print ISBN:
9780121820602

About the serial-volume-editors

Jackie Corbin

Affiliations and Expertise

Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.

Roger Johnson

Affiliations and Expertise

School of Medicine Health Sciences Center, University of New York, Stony Brook, U.S.A.

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY