In VIVO Perfusion and Release of Neroactive substances

In VIVO Perfusion and Release of Neroactive substances

Methods and Strategies

1st Edition - January 28, 1985

Write a review

  • Editor: Alejandro Bayon
  • eBook ISBN: 9780323155229

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order

Description

In Vivo Perfusion and Release of Neuroactive Substances: Methods and Strategies examines the perfusion and release methods and strategies used to study in vivo neurochemistry in relation to electrophysiological and behavioral events. More specifically, it reviews methodological alternatives and experimental strategies for investigating the in vivo perfusion and release of brain substances in the central nervous system. It also assesses the potential and limitations of in vivo perfusion methods, including high-pressure liquid chromatography and highly sensitive as well as specific immunoassays and receptor assays, as a means to discover novel neurochemicals recovered from perfusates. Organized into two sections comprised of 17 chapters, this book begins with an overview of methodological alternatives and experimental strategies that are deemed critical for the outcome of research on the perfusion and release of neuroactive substances in the CNS. It then discusses the personal experiences of the scientists in studying the in vivo neurochemistry and its correlation with other neural events. The chapters that follow focus on the chemical, bioelectrical, and behavioral aspects of brain function; interdisciplinary techniques for approaching problems of neuronal connectivity, neuroendocrinology, motor behavior, neuropathology, etc.; technical issues associated with push-pull perfusion in unanesthetized and unrestrained experimental animals; hippocampal involvement in the effects of ethyl alcohol; and the use of the push-pull perfusion technique in neuroendocrinology. This book will be of interest to specialists and neuroscientists as well as students working in the fields of neuroscience, neurochemistry, and neuroendocrinology.

Table of Contents


  • Contributors

    Preface

    1 Introduction

    Methodological Alternatives and Experimental Strategies for Studying the in Vivo Perfusion and Release of Neuroactive Substances in the Central Nervous System

    References

    Part I Methods

    2 Technical Issues Associated with Push-Pull Perfusion in Unanesthetized and Unrestrained Animals

    I. Introduction

    II. Methods

    III. Findings

    IV. Perspectives and Discussion

    References

    3 Neuronal Responses to Neuroactive Substances Administered by Push-Pull Perfusion

    I. Introduction

    II. Methods

    III. Findings

    IV. Effects of Dopamine on Striatal Neuron Discharge

    V. Ethanol Action on Striatal Neurons

    VI. Perspectives and Discussion

    References

    4 Superfusion and Electrical Stimulation of the Canine Brain with a Push-Pull Cannula

    I. Introduction

    II. Methods

    III. Findings and Optimization of Experimental Procedures

    IV. Conclusion

    References

    5 Push-Pull Perfusion Studies on the in Vivo Release of Endogenous Transmitter and Nontransmitter Amino Acids in the Rat Brain

    I. Introduction

    II. Methods

    III. Findings

    IV. Perspectives and Discussion

    References

    6 Push-Pull Perfusion Studies on the in Vivo Release of Proteins, Enzymes, and the Neuroactive Peptides-Enkephalins from the Rat Brain

    I. Introduction

    II. Methods

    III. Findings

    IV. Perspectives

    References

    7 Use of Dialytrodes in Brain Neurochemistry

    I. Introduction

    II. Methods

    III. Findings

    IV. Perspectives and Discussion

    References

    8 In Vivo Brain Dialysis of Extracellular Nontransmitter and Putative Transmitter Amino Acids

    I. Introduction

    II. Methods

    III. Findings

    IV. Perspectives

    References

    9 Voltammetry Measurement in Vivo of Neurotransmitter Release in the Freely Moving Rat

    I. Introduction

    II. Methods

    III. Findings

    IV. Perspectives and Discussion

    References

    Part II Strategies

    10 Dopamine Release and Neuronal Activity in the Two Nigrostriatal Systems

    I. Introduction

    II. Methodological Considerations

    III. Changes in Dopamine Release in Relation Nerve Impulse Flow

    IV. Dopamine Release from Dendrites of the Nigrostriatal Dopaminergic Neurons

    V. Reciprocal Control of the Activity of the Two Nigrostriatal Dopaminergic Pathways

    VI. Presynaptic Regulation of Striatal Dopamine Release

    VII. Conclusion and Perspectives

    References

    11 Pharmacologically Induced Changes in the Efflux of Metabolites of Dopamine and 5-Hydroxytryptamine from the Brains of Freely Moving Rats

    I. Methods

    II. Findings

    III. Perspectives

    References

    12 Hippocampal Involvement in the Effects of Ethyl Alcohol: In Vivo and in Vitro Levels of Analysis

    I. Introduction

    II. Overview of Hippocampal Anatomy

    III. Findings

    IV. Summary and Conclusions

    References

    13 The Use of Push-Pull Cannulae for Superfusing Various Hypothalamic Areas in Anesthetized and Conscious, Freely Moving Animals

    I. Introduction

    II. Methods

    III. Findings

    IV. Perspectives

    References

    14 Release of Arginine Vasopressin from the Brain: Correlation with Physiological Events

    I. Introduction

    II. Methods

    III. Findings

    IV. Discussion

    References

    15 The Push-Pull Perfusion Technique in Neuroendocrinology

    I. Introduction

    II. Methods

    III. Findings

    IV. Perspectives and Conclusions

    References

    16 The Efficacy of the Push-Pull Perfusion Technique in the Study of Sleep, Epilepsy, and Tremor

    I. Introduction

    II. Methods

    III. Findings

    IV. Perspectives and Discussion

    References

    17 The Use of Push-Pull Perfusion Techniques for Measuring the Efflux of [3H] Dopamine in the Striatum of the Conscious Rat

    I. Introduction

    II. Methods

    III. Findings

    IV. Discussion and Perspectives

    References

    Index




Product details

  • No. of pages: 340
  • Language: English
  • Copyright: © Academic Press 1985
  • Published: January 28, 1985
  • Imprint: Academic Press
  • eBook ISBN: 9780323155229

About the Editor

Alejandro Bayon

Ratings and Reviews

Write a review

There are currently no reviews for "In VIVO Perfusion and Release of Neroactive substances"