In VIVO Perfusion and Release of Neroactive substances

Methods and Strategies

1st Edition - January 28, 1985
Editor: Alejandro Bayon
Language: English
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 5 5 2 2 - 9

In Vivo Perfusion and Release of Neuroactive Substances: Methods and Strategies examines the perfusion and release methods and strategies used to study in vivo neurochemistry in… Read more

In VIVO Perfusion and Release of Neroactive substances

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In Vivo Perfusion and Release of Neuroactive Substances: Methods and Strategies examines the perfusion and release methods and strategies used to study in vivo neurochemistry in relation to electrophysiological and behavioral events. More specifically, it reviews methodological alternatives and experimental strategies for investigating the in vivo perfusion and release of brain substances in the central nervous system. It also assesses the potential and limitations of in vivo perfusion methods, including high-pressure liquid chromatography and highly sensitive as well as specific immunoassays and receptor assays, as a means to discover novel neurochemicals recovered from perfusates. Organized into two sections comprised of 17 chapters, this book begins with an overview of methodological alternatives and experimental strategies that are deemed critical for the outcome of research on the perfusion and release of neuroactive substances in the CNS. It then discusses the personal experiences of the scientists in studying the in vivo neurochemistry and its correlation with other neural events. The chapters that follow focus on the chemical, bioelectrical, and behavioral aspects of brain function; interdisciplinary techniques for approaching problems of neuronal connectivity, neuroendocrinology, motor behavior, neuropathology, etc.; technical issues associated with push-pull perfusion in unanesthetized and unrestrained experimental animals; hippocampal involvement in the effects of ethyl alcohol; and the use of the push-pull perfusion technique in neuroendocrinology. This book will be of interest to specialists and neuroscientists as well as students working in the fields of neuroscience, neurochemistry, and neuroendocrinology.

Contributors

Preface

1 Introduction

Methodological Alternatives and Experimental Strategies for Studying the in Vivo Perfusion and Release of Neuroactive Substances in the Central Nervous System

References

Part I Methods

2 Technical Issues Associated with Push-Pull Perfusion in Unanesthetized and Unrestrained Animals

I. Introduction

II. Methods

III. Findings

IV. Perspectives and Discussion

References

3 Neuronal Responses to Neuroactive Substances Administered by Push-Pull Perfusion

I. Introduction

II. Methods

III. Findings

IV. Effects of Dopamine on Striatal Neuron Discharge

V. Ethanol Action on Striatal Neurons

VI. Perspectives and Discussion

References

4 Superfusion and Electrical Stimulation of the Canine Brain with a Push-Pull Cannula

I. Introduction

II. Methods

III. Findings and Optimization of Experimental Procedures

IV. Conclusion

References

5 Push-Pull Perfusion Studies on the in Vivo Release of Endogenous Transmitter and Nontransmitter Amino Acids in the Rat Brain

I. Introduction

II. Methods

III. Findings

IV. Perspectives and Discussion

References

6 Push-Pull Perfusion Studies on the in Vivo Release of Proteins, Enzymes, and the Neuroactive Peptides-Enkephalins from the Rat Brain

I. Introduction

II. Methods

III. Findings

IV. Perspectives

References

7 Use of Dialytrodes in Brain Neurochemistry

I. Introduction

II. Methods

III. Findings

IV. Perspectives and Discussion

References

8 In Vivo Brain Dialysis of Extracellular Nontransmitter and Putative Transmitter Amino Acids

I. Introduction

II. Methods

III. Findings

IV. Perspectives

References

9 Voltammetry Measurement in Vivo of Neurotransmitter Release in the Freely Moving Rat

I. Introduction

II. Methods

III. Findings

IV. Perspectives and Discussion

References

Part II Strategies

10 Dopamine Release and Neuronal Activity in the Two Nigrostriatal Systems

I. Introduction

II. Methodological Considerations

III. Changes in Dopamine Release in Relation Nerve Impulse Flow

IV. Dopamine Release from Dendrites of the Nigrostriatal Dopaminergic Neurons

V. Reciprocal Control of the Activity of the Two Nigrostriatal Dopaminergic Pathways

VI. Presynaptic Regulation of Striatal Dopamine Release

VII. Conclusion and Perspectives

References

11 Pharmacologically Induced Changes in the Efflux of Metabolites of Dopamine and 5-Hydroxytryptamine from the Brains of Freely Moving Rats

I. Methods

II. Findings

III. Perspectives

References

12 Hippocampal Involvement in the Effects of Ethyl Alcohol: In Vivo and in Vitro Levels of Analysis

I. Introduction

II. Overview of Hippocampal Anatomy

III. Findings

IV. Summary and Conclusions

References

13 The Use of Push-Pull Cannulae for Superfusing Various Hypothalamic Areas in Anesthetized and Conscious, Freely Moving Animals

I. Introduction

II. Methods

III. Findings

IV. Perspectives

References

14 Release of Arginine Vasopressin from the Brain: Correlation with Physiological Events

I. Introduction

II. Methods

III. Findings

IV. Discussion

References

15 The Push-Pull Perfusion Technique in Neuroendocrinology

I. Introduction

II. Methods

III. Findings

IV. Perspectives and Conclusions

References

16 The Efficacy of the Push-Pull Perfusion Technique in the Study of Sleep, Epilepsy, and Tremor

I. Introduction

II. Methods

III. Findings

IV. Perspectives and Discussion

References

17 The Use of Push-Pull Perfusion Techniques for Measuring the Efflux of [3H] Dopamine in the Striatum of the Conscious Rat

I. Introduction

II. Methods

III. Findings

IV. Discussion and Perspectives

References

Index