In Vitro Biological Systems

In Vitro Biological Systems

Methods in Toxicology, Vol. 1

1st Edition - January 1, 1993

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  • Editors: Charles A. Tyson, John M. Frazier
  • eBook ISBN: 9781483218601

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Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable.

Table of Contents

  • Contributors



    Section I Neural and Neuromuscular Systems

    1. Hippocampal Slices

    2. Organotypic Neural Cultures

    3. Reaggregate Cultures for Neurotoxicological Studies

    4. Cytotoxicity in Murine Neocortical Cell Culture

    5. Cultures of Adult Trigeminal Ganglion Neurons

    6. Adrenomedullary Chromaffin Cells for Toxicological Studies

    7. PC-12 Cells

    Section II Ocular System

    8. Corneal Epithelial and Endothelial Cell Culture

    9. Isolation and Culture of Retinal Epithelium

    Section III Respiratory System

    10. Isolation and Culture of Type II Alveolar Epithelial Cells

    11. Isolation of Nonciliated Bronchiolar (Clara) Epithelial Cells from Mouse Lung

    12. Rat Tracheal Epithelial Cell Cultures for Studies in Toxicology and Carcinogenicity

    Section IV Cardiovascular System

    13. Preparation of Primary Cultures of Postnatal Rat Myocardial Cells for Toxicological Studies

    14. Aortic Endothelial and Smooth Muscle Cell Cultures

    15. Vessel Cylinders

    Section V Gastrointestinal System

    16. Gastric Mucosal Cell Culture for Toxicological Study

    17. Small Intestinal Enterocytes

    18. Maintenance and Characterization of an Organ Culture System for Colonic Mucosa

    Section VIA Liver Slices

    19. Precision-Cut Rat Liver Slices in Dynamic Organ Culture for Structure-Toxicity Studies

    Section VIB Liver: Hepatocytes

    20. Isolation of Hepatocytes by Collagenase Perfusion

    21. Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells for Hepatotoxic Assessment of Xenobiotics

    22. Isolation and Culture of Hepatocytes from Different Laboratory Species

    23. Isolation of Human Hepatocytes by Biopsy Perfusion Methods

    24. Human Hepatocyte Cultures

    Section VIC Liver: Other Cell Systems

    25. Hepatocyte and Spleen Cell Systems

    26. Isolation and Culture of Hepatic Nonparenchymal Cells

    27. Proliferating Lines of Rat Liver Epithelial Cells

    28. Isolation and Culture of Liver Epithelial Cells from Carcinogen-Treated Rats

    Section VIIA Kidney: Proximal Tubule Fragments

    29. Renal Proximal Tubule Preparations Isolated from Rat or Rabbit Kidneys without the Use of Collagenase

    30. Suspension Culture of Rabbit Renal Proximal Tubules

    31. Perfusion Method for Isolation of Renal Proximal Tubules from Mammalian Species

    32. Isolation and Maintenance of Milligram Quantities of Rabbit Renal Proximal Straight and Convoluted Tubules

    Section VIIB Kidney Cell Culture

    33. Rabbit Cortical Epithelial Cells

    34. Culture of Rat Kidney Proximal Tubule Epithelial Cells in Defined Medium

    35. Primary Culture of Rat Renal Medullary Interstitial Cells

    36. Purification of Renal Cortical Cell Populations by Percoll Density-Gradient Centrifugation

    37. Renal Cell Lines Derived from Rabbit and Pig Kidney

    Section VIII Reproductive and Developmental Systems

    38. Whole Embryo Culture: Organogenesis of Rodent Embryos in Vitro

    39. Preparation and Use of Sertoli-Germ Cell Cocultures from 28-Day-Old Rats

    Section IX Immune System

    40. Isolation and in Vitro Culture of Mononuclear Phagocytes

    41. T-Lymphocyte Assays

    42. B Lymphocytes: Comparison of Murine and Human B-Cell Immunocompetence

    43. Bone Marrow Stromal Cell Cultures

    Section X Skin

    44. Penetration Studies with Excised Human and Animal Skin

    45. Porcine Skin Flaps

    46. Keratinocytes


Product details

  • No. of pages: 592
  • Language: English
  • Copyright: © Academic Press 1993
  • Published: January 1, 1993
  • Imprint: Academic Press
  • eBook ISBN: 9781483218601

About the Editors

Charles A. Tyson

John M. Frazier

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