In Vitro Biological Systems - 1st Edition - ISBN: 9780124612013, 9781483218601

In Vitro Biological Systems

1st Edition

Methods in Toxicology, Vol. 1

Editors: Charles A. Tyson John M. Frazier
eBook ISBN: 9781483218601
Imprint: Academic Press
Published Date: 21st January 1993
Page Count: 592
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Description

Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable.

Table of Contents


Contributors

Preface

Introduction

Section I Neural and Neuromuscular Systems

1. Hippocampal Slices

2. Organotypic Neural Cultures

3. Reaggregate Cultures for Neurotoxicological Studies

4. Cytotoxicity in Murine Neocortical Cell Culture

5. Cultures of Adult Trigeminal Ganglion Neurons

6. Adrenomedullary Chromaffin Cells for Toxicological Studies

7. PC-12 Cells

Section II Ocular System

8. Corneal Epithelial and Endothelial Cell Culture

9. Isolation and Culture of Retinal Epithelium

Section III Respiratory System

10. Isolation and Culture of Type II Alveolar Epithelial Cells

11. Isolation of Nonciliated Bronchiolar (Clara) Epithelial Cells from Mouse Lung

12. Rat Tracheal Epithelial Cell Cultures for Studies in Toxicology and Carcinogenicity

Section IV Cardiovascular System

13. Preparation of Primary Cultures of Postnatal Rat Myocardial Cells for Toxicological Studies

14. Aortic Endothelial and Smooth Muscle Cell Cultures

15. Vessel Cylinders

Section V Gastrointestinal System

16. Gastric Mucosal Cell Culture for Toxicological Study

17. Small Intestinal Enterocytes

18. Maintenance and Characterization of an Organ Culture System for Colonic Mucosa

Section VIA Liver Slices

19. Precision-Cut Rat Liver Slices in Dynamic Organ Culture for Structure-Toxicity Studies

Section VIB Liver: Hepatocytes

20. Isolation of Hepatocytes by Collagenase Perfusion

21. Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells for Hepatotoxic Assessment of Xenobiotics

22. Isolation and Culture of Hepatocytes from Different Laboratory Species

23. Isolation of Human Hepatocytes by Biopsy Perfusion Methods

24. Human Hepatocyte Cultures

Section VIC Liver: Other Cell Systems

25. Hepatocyte and Spleen Cell Systems

26. Isolation and Culture of Hepatic Nonparenchymal Cells

27. Proliferating Lines of Rat Liver Epithelial Cells

28. Isolation and Culture of Liver Epithelial Cells from Carcinogen-Treated Rats

Section VIIA Kidney: Proximal Tubule Fragments

29. Renal Proximal Tubule Preparations Isolated from Rat or Rabbit Kidneys without the Use of Collagenase

30. Suspension Culture of Rabbit Renal Proximal Tubules

31. Perfusion Method for Isolation of Renal Proximal Tubules from Mammalian Species

32. Isolation and Maintenance of Milligram Quantities of Rabbit Renal Proximal Straight and Convoluted Tubules

Section VIIB Kidney Cell Culture

33. Rabbit Cortical Epithelial Cells

34. Culture of Rat Kidney Proximal Tubule Epithelial Cells in Defined Medium

35. Primary Culture of Rat Renal Medullary Interstitial Cells

36. Purification of Renal Cortical Cell Populations by Percoll Density-Gradient Centrifugation

37. Renal Cell Lines Derived from Rabbit and Pig Kidney

Section VIII Reproductive and Developmental Systems

38. Whole Embryo Culture: Organogenesis of Rodent Embryos in Vitro

39. Preparation and Use of Sertoli-Germ Cell Cocultures from 28-Day-Old Rats

Section IX Immune System

40. Isolation and in Vitro Culture of Mononuclear Phagocytes

41. T-Lymphocyte Assays

42. B Lymphocytes: Comparison of Murine and Human B-Cell Immunocompetence

43. Bone Marrow Stromal Cell Cultures

Section X Skin

44. Penetration Studies with Excised Human and Animal Skin

45. Porcine Skin Flaps

46. Keratinocytes

Index


Details

No. of pages:
592
Language:
English
Copyright:
© Academic Press 1993
Published:
Imprint:
Academic Press
eBook ISBN:
9781483218601

About the Editor

Charles A. Tyson

John M. Frazier