In Vitro Biological Systems
Methods in Toxicology, Vol. 1
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Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable.
Table of Contents
Contributors
Preface
Introduction
Section I Neural and Neuromuscular Systems
1. Hippocampal Slices
2. Organotypic Neural Cultures
3. Reaggregate Cultures for Neurotoxicological Studies
4. Cytotoxicity in Murine Neocortical Cell Culture
5. Cultures of Adult Trigeminal Ganglion Neurons
6. Adrenomedullary Chromaffin Cells for Toxicological Studies
7. PC-12 Cells
Section II Ocular System
8. Corneal Epithelial and Endothelial Cell Culture
9. Isolation and Culture of Retinal Epithelium
Section III Respiratory System
10. Isolation and Culture of Type II Alveolar Epithelial Cells
11. Isolation of Nonciliated Bronchiolar (Clara) Epithelial Cells from Mouse Lung
12. Rat Tracheal Epithelial Cell Cultures for Studies in Toxicology and Carcinogenicity
Section IV Cardiovascular System
13. Preparation of Primary Cultures of Postnatal Rat Myocardial Cells for Toxicological Studies
14. Aortic Endothelial and Smooth Muscle Cell Cultures
15. Vessel Cylinders
Section V Gastrointestinal System
16. Gastric Mucosal Cell Culture for Toxicological Study
17. Small Intestinal Enterocytes
18. Maintenance and Characterization of an Organ Culture System for Colonic Mucosa
Section VIA Liver Slices
19. Precision-Cut Rat Liver Slices in Dynamic Organ Culture for Structure-Toxicity Studies
Section VIB Liver: Hepatocytes
20. Isolation of Hepatocytes by Collagenase Perfusion
21. Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells for Hepatotoxic Assessment of Xenobiotics
22. Isolation and Culture of Hepatocytes from Different Laboratory Species
23. Isolation of Human Hepatocytes by Biopsy Perfusion Methods
24. Human Hepatocyte Cultures
Section VIC Liver: Other Cell Systems
25. Hepatocyte and Spleen Cell Systems
26. Isolation and Culture of Hepatic Nonparenchymal Cells
27. Proliferating Lines of Rat Liver Epithelial Cells
28. Isolation and Culture of Liver Epithelial Cells from Carcinogen-Treated Rats
Section VIIA Kidney: Proximal Tubule Fragments
29. Renal Proximal Tubule Preparations Isolated from Rat or Rabbit Kidneys without the Use of Collagenase
30. Suspension Culture of Rabbit Renal Proximal Tubules
31. Perfusion Method for Isolation of Renal Proximal Tubules from Mammalian Species
32. Isolation and Maintenance of Milligram Quantities of Rabbit Renal Proximal Straight and Convoluted Tubules
Section VIIB Kidney Cell Culture
33. Rabbit Cortical Epithelial Cells
34. Culture of Rat Kidney Proximal Tubule Epithelial Cells in Defined Medium
35. Primary Culture of Rat Renal Medullary Interstitial Cells
36. Purification of Renal Cortical Cell Populations by Percoll Density-Gradient Centrifugation
37. Renal Cell Lines Derived from Rabbit and Pig Kidney
Section VIII Reproductive and Developmental Systems
38. Whole Embryo Culture: Organogenesis of Rodent Embryos in Vitro
39. Preparation and Use of Sertoli-Germ Cell Cocultures from 28-Day-Old Rats
Section IX Immune System
40. Isolation and in Vitro Culture of Mononuclear Phagocytes
41. T-Lymphocyte Assays
42. B Lymphocytes: Comparison of Murine and Human B-Cell Immunocompetence
43. Bone Marrow Stromal Cell Cultures
Section X Skin
44. Penetration Studies with Excised Human and Animal Skin
45. Porcine Skin Flaps
46. Keratinocytes
Index
Product details
- No. of pages: 592
- Language: English
- Copyright: © Academic Press 1993
- Published: January 1, 1993
- Imprint: Academic Press
- eBook ISBN: 9781483218601