Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-expression Analyses, Second Edition, continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other "cook-book" type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry.
Explains the theory and foundation of immunohistochemistry and in situ hybridization and presents easy-to-follow experimental protocols with tricks of the trade
Includes two new chapters: Recent improvements in immunohistochemistry and in situ hybridization, Quality control for immunohistochemistry and in situ hybridization: How to know if the color change is signal or background
The second edition also includes a detailed test to help one learn the basics of histologic interpretation of tissues and a separate detailed test in how to differentiate signal from background
Includes chapter-ending summaries of Key Points to Remember, bringing beginners up to speed with any seasoned veteran in the field
Thoughtfully tackles the molecular basis if IHC and ISH, along with application of that knowledge to improving the techniques is significant
Academic researchers, diagnostic pathologists, clinical chemists, practicing clinicians, toxicologists, biopharmaceutical researchers, and private pathology laboratories that perform either in situ hybridization, immunohistochemistry, or co-expression analyses
Table of Contents
1. Introduction 2. The Basics of Molecular Pathology 3. The Biochemical Basis of in situ Hybridization and Immunohistochemistry 4. The Basics of in situ Hybridization 5. The Basics of Immunohistochemistry 6. Quality Control for Immunohistochemistry and in situ Hybridization: How to Know if the Color Change Is Signal or Background 7. Recent Improvements in Immunohistochemistry and in situ Hybridization 8. The Basics of Histologic Interpretations of Tissues 9. The Recommended Protocol for in situ Hybridization 10. The Recommended Protocol for Immunohistochemistry 11. Coexpression Analyses 12. PCR in situ Hybridization and RT in situ PCR
Dr. Gerard Nuovo received his MD from the University of Vermont College of Medicine in 1983. He did his training in Anatomic Pathology at Columbia Prebyterian College of Physicians and Surgeons in NYC. He then did a two year fellowship at Columbia Presbyterian in Molecular Virology and Gynecologic Pathology under the mentorship of Drs. Saul Silverstein, Chris Crum, and Ralph Richart. Dr. Nuovo’s career has been dedicated to bridging the areas of surgical pathology and molecular pathology. He has published over 400 peer review papers in the field and written several textbooks in both Anatomic Pathology and Molecular Pathology. This textbook fulfills a strong ambition of Dr. Nuovo; to empower the next generation of researchers and diagnosticians to include in situ molecular pathology in their work by using easy-to-follow and easy-to-understand protocols for in situ hybridization, immunohistochemistry, and co-expression analyses.
Affiliations and Expertise
Ohio State University Medical Center, Columbus, OH, USA