Immunology of Infection

Immunology of Infection

2nd Edition - November 25, 2002

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  • Editors: Stefan Kaufmann, Dieter Kabelitz
  • Paperback ISBN: 9780124020337

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Immunology of Infection, 2nd Edition, edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist interested in analysing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology.This new edition of Immunology of Infection provides ready-to-use "recipes", and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the successful first edition.Methods in Microbiologyis the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.

Key Features

  • Includes techniques for genome-wide expression profiling of both the pathogen and host and of the host response to infection
  • Cytometric analysis of cytokine secretion by immune cells
  • Describes tetramer technology for the quantitative analysis of antigen specific T cell responses
  • Analysis of host cells and pathogens involved in the host-microbe interplay
  • Covers techniques useful for the analysis of human and murine systems
  • Includes techniques for the prediction and determination of MHC ligands and T cell epitopes
  • Covers the fundamentals and practice of DNA vaccines
  • Describes methods for the isolation and propagation of human dendritic cells


Microbiologists, immunologists, mycologists, and parasitologists

Table of Contents

  • Introduction
    The immune response to infectious agents

    General Methods
    Phenotyping and separation of leukocyte populations based on affinity labelling
    Cytometric cytokine secretion assay: detection and isolation of cytokine secreting T cells
    Measurement of cellular proliferation
    Use of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine design
    The use of tetramers in the quantitative analysis of T cell responses
    Using microarrays for studying the host transcriptional response to microbial infection
    Genome-wide expression profiling of intracelllular bacteria: the interaction of Mycobacterium tuberculosis with macrophages

    Murine Models
    Management of immuno-compromised and infected animals
    In Vitro Analysis
    Isolation and preparation of lymphocytes from infected animals for in vitro analysis
    Isolation and development of murine T-lymphocyte hybridomas and clonal cell lines
    Killer cell assays
    Quantitation of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry and Reverse Transcriptase-PCR Methods
    Isolation of and measuring the function of professional phagocytes: murine macrophages
    In vivo Analysis
    The immune response in mice challenged with bacterial infections
    Measuring immune responses in vivo
    Specific Models
    Murine model of tuberculosis
    The leishmaniasis model
    Animal models: murine cytomegalovirus
    DNA vaccines: fundamentals and practice
    Preparation and use of adjuvants

    Human Systems
    Isolation and propagation of human dendritic cells
    Isolation of T cells and establishment of T-cell lines and clones
    Growth-transformation of human T cells
    Generation and characterisation of human killer cells
    Measuring human cytokine responses
    Measuring immune responses in situ: immunofluorescent and immunoenzymatic techniques
    Isolation, characterisation and cultivation of
    human monocytes and macrophages

Product details

  • No. of pages: 824
  • Language: English
  • Copyright: © Academic Press 2002
  • Published: November 25, 2002
  • Imprint: Academic Press
  • Paperback ISBN: 9780124020337

About the Series Volume Editors

Stefan Kaufmann

Affiliations and Expertise

Max Planck Institute for Infection Biology, Berlin, Germany

Dieter Kabelitz

Dieter Kabelitz is full professor at the University of Kiel, Germany, and Director of the Institute of Immunology. Dieter Kabelitz studied medicine in Freiburg and Munich, Germany. He was a post-doc in Uppsala/Sweden and at Rockefeller University, New York. In 1988, he was appointed Associate Professor for Cellular Immunology at the University of Heidelberg. From 1992 to 1999 he was Head of the Department of Immunology at the Paul-Ehrlich Institute in Langen, Germany. Since 1999 he is a full professor at the University of Kiel and Director of the Institute of Immunology. His scientific expertise is in the field of the functional characterization of human T cells with a special focus on  T cells. Current projects aim to explore the potential of human  T cells for the immunotherapy of cancer. He has published more than 360 papers listed in PubMed/Medline. Dieter Kabelitz was chairman of the Collaborative Research Center (SFB) 415 „Specificity and Pathophysiology of Signal Transduction Pathways“ funded at Kiel University by the German Research Foundation (DFG). He is a founding member and member of the Steering Committee of the DFG-funded Cluster of Excellence „Inflammation-at-Interfaces“. He was President of the German Society for Immunology (DGfI) in 2011 and 2012, and he is a Council member of the International Union of Immunological Societies (IUIS) and chair of the Education Committee (EDU) of IUIS.

Affiliations and Expertise

Institute of Immunology, Christian-Albrechts-University, Kiel, Germany

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