Immunology of Infection - 2nd Edition - ISBN: 9780125215329, 9780080574820

Immunology of Infection, Volume 32

2nd Edition

Series Volume Editors: Stefan Kaufmann Dieter Kabelitz
eBook ISBN: 9780080574820
eBook ISBN: 9780080925028
Hardcover ISBN: 9780125215329
Paperback ISBN: 9780124020337
Imprint: Academic Press
Published Date: 25th November 2002
Page Count: 824
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Table of Contents

Contributors Preface to the 2nd edition Preface to the 1st edition

Introduction The immune response to infectious agents

General Methods Phenotyping and separation of leukocyte populations based on affinity labelling Cytometric cytokine secretion assay: detection and isolation of cytokine secreting T cells Measurement of cellular proliferation Use of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine design The use of tetramers in the quantitative analysis of T cell responses Using microarrays for studying the host transcriptional response to microbial infection Genome-wide expression profiling of intracelllular bacteria: the interaction of Mycobacterium tuberculosis with macrophages

Murine Models Management of immuno-compromised and infected animals

In Vitro Analysis Isolation and preparation of lymphocytes from infected animals for in vitro analysis Isolation and development of murine T-lymphocyte hybridomas and clonal cell lines Killer cell assays Quantitation of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry and Reverse Transcriptase-PCR Methods Isolation of and measuring the function of professional phagocytes: murine macrophages

In vivo Analysis The immune response in mice challenged with bacterial infections Measuring immune responses in vivo

Specific Models Murine model of tuberculosis The leishmaniasis model Animal models: murine cytomegalovirus DNA vaccines: fundamentals and practice Preparation and use of adjuvants

Human Systems Isolation and propagation of human dendritic cells Isolation of T cells and establishment of T-cell lines and clones Growth-transformation of human T cells Generation and characterisation of human killer cells Measuring human cytokine responses Measuring immune responses in situ: immunofluorescent and immunoenzymatic techniques Isolation, characterisation and cultivation of human monocytes and macrophages


Immunology of Infection, 2nd Edition, edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist interested in analysing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology.

This new edition of Immunology of Infection provides ready-to-use "recipes", and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the successful first edition.

Methods in Microbiologyis the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.

Key Features

  • Includes techniques for genome-wide expression profiling of both the pathogen and host and of the host response to infection
  • Cytometric analysis of cytokine secretion by immune cells
  • Describes tetramer technology for the quantitative analysis of antigen specific T cell responses
  • Analysis of host cells and pathogens involved in the host-microbe interplay
    Covers techniques useful for the analysis of human and murine systems
    Includes techniques for the prediction and determination of MHC ligands and T cell epitopes
    Covers the fundamentals and practice of DNA vaccines 
    Describes methods for the isolation and propagation of human dendritic cells


Microbiologists, immunologists, mycologists, and parasitologists


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© Academic Press 2003
Academic Press
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Praise for the first edition:
"This is a useful compendium of methods and techniques for those wishing to examine the immune responses to infections in vitro and in vivo. The book is clearly presented, packed with protocols, and well illustrated" --Microbiology Today

" represents a valuable addition to the microbiologists' reference source" --Immunology Today

Praise for the Series:
"An invaluable resource for the detailed analysis of cellular gene function and genome architecture." --NATURE

Ratings and Reviews

About the Series Volume Editors

Stefan Kaufmann Series Volume Editor

Affiliations and Expertise

Max Planck Institute for Infection Biology, Berlin, Germany

Dieter Kabelitz Series Volume Editor

Affiliations and Expertise

University of Kiel, Germany