Immunology of Infection - 1st Edition - ISBN: 9780124023055, 9780080860541

Immunology of Infection, Volume 25

1st Edition

Serial Volume Editors: Stefan Kaufmann Dieter Kabelitz
Paperback ISBN: 9780124023055
eBook ISBN: 9780080860541
Imprint: Academic Press
Published Date: 21st May 1998
Page Count: 705
Tax/VAT will be calculated at check-out

Institutional Access


Table of Contents

Introduction:

S.H.E. Kaufmann and D. Kabelitz, The Immune Response to Infectious Agents.

General Methods:

A. Scheffold and A. Radbruch, Phenotyping and Separation of Leukocyte Populations.

D. Kabelitz and K. Pechold, Measurement of Cellular Proliferation (3H-TdR, MTT, Absolute Cell Numbers by SCUD).

Murine Models:

H. Mossmann, Management of Infected and Immunocompromised Animals.

C.J. Czuprynski and J.F. Brown, In Vitro Analysis.

W. Born, M. Tsuji, C. Cady, M. Lahn, A. Mukasa, C. Reardon, and R. O'Brien, Establishment of Murine T Cell Lines, T Cell Clones, Hybridomas and Transfectomas.

D.H. Busch and E.G. Pamer, Killer Cell Assays.

R. Haworth and S. Gordon, Isolation of Professional Phagocytes and Measuring their Functions: Murine Macrophages in Vitro.

M. Mielke, In Vivo Analyses.

S. Ehlers and U. Seitzer, Measuring Immune Responses in Vivo.

A.D. Roberts, J.T. Belisle, A.M. Cooper, and I.M. Orme, Specific Models.

P. Kropf, K. Brunson, R. Etges, and I. Muller, The Leishmaniasis Model.

J.B. Ulmer, J.J. Donnelly, D.L. Montgomery, and M.A. Liu, Art and Science of DNA Vaccines.

K. Lövgren-Bengtsson, Preparation and Use of Adjuvants.

Human System:

J. Banchereau, Isolation and Propagation of Dendritic Cells.

E. Märker-Hermann and R. Duchmann, Isolation of T Cells and Establishment of T Cell Lines and Clones.

H. Fickenscher and B. Fleckenstein, Growth-Transformation of Human T Cells.

G. Pawelec, E. Mariani, R. Offringa, C.J.M. Melief, and R. Solana, Generation and Characterization of Killer Cells.

H. Yssel and F. Cottrez, Measuring Human Cytokine Responses.

U. Seitzer and J. Gerdes, Measuring Immune Responses in Situ: Immunohistology and in Situ Hybridization.

S.W. Krause, M. Kreutz, and R. Andreesen, Isolation, Characterization, and Cultivation of Human Monocytes and Macrophages.


Description

Introduction:

S.H.E. Kaufmann and D. Kabelitz, The Immune Response to Infectious Agents.

General Methods:

A. Scheffold and A. Radbruch, Phenotyping and Separation of Leukocyte Populations.

D. Kabelitz and K. Pechold, Measurement of Cellular Proliferation (3H-TdR, MTT, Absolute Cell Numbers by SCUD).

Murine Models:

H. Mossmann, Management of Infected and Immunocompromised Animals.

C.J. Czuprynski and J.F. Brown, In Vitro Analysis.

W. Born, M. Tsuji, C. Cady, M. Lahn, A. Mukasa, C. Reardon, and R. O'Brien, Establishment of Murine T Cell Lines, T Cell Clones, Hybridomas and Transfectomas.

D.H. Busch and E.G. Pamer, Killer Cell Assays.

R. Haworth and S. Gordon, Isolation of Professional Phagocytes and Measuring their Functions: Murine Macrophages in Vitro.

M. Mielke, In Vivo Analyses.

S. Ehlers and U. Seitzer, Measuring Immune Responses in Vivo.

A.D. Roberts, J.T. Belisle, A.M. Cooper, and I.M. Orme, Specific Models.

P. Kropf, K. Brunson, R. Etges, and I. Muller, The Leishmaniasis Model.

J.B. Ulmer, J.J. Donnelly, D.L. Montgomery, and M.A. Liu, Art and Science of DNA Vaccines.

K. Lövgren-Bengtsson, Preparation and Use of Adjuvants.

Human System:

J. Banchereau, Isolation and Propagation of Dendritic Cells.

E. Märker-Hermann and R. Duchmann, Isolation of T Cells and Establishment of T Cell Lines and Clones.

H. Fickenscher and B. Fleckenstein, Growth-Transformation of Human T Cells.

G. Pawelec, E. Mariani, R. Offringa, C.J.M. Melief, and R. Solana, Generation and Characterization of Killer Cells.

H. Yssel and F. Cottrez, Measuring Human Cytokine Responses.

U. Seitzer and J. Gerdes, Measuring Immune Responses in Situ: Immunohistology and in Situ Hybridization.

S.W. Krause, M. Kreutz, and R. Andreesen, Isolation, Characterization, and Cultivation of Human Monocytes and Macrophages.

Key Features

@introbul:Key Features @bul:* Focuses on the methods most useful for the microbiologist interested in analysing host-pathogen relationships

  • Ready-to-use, tried and tested recipes
  • Lists of suppliers provided as appendices to each chapter
  • Covers techniques useful for the analysis of human and murine cells
  • Includes techniques for the prediction and determination of MHC ligands and T cell epitopes
  • Describes the art and science of DNA vaccines
  • Essential methods for measuring human cytokine responses
  • Covers isolation and propagation of dendritic cells

Readership

Microbiologists who use immunological techniques during their study of microorganisms, especially those studying infectious agents and infectious disease, clinical microbiologists, immunologists, and parasitologists.


Details

No. of pages:
705
Language:
English
Copyright:
© Academic Press 1998
Published:
Imprint:
Academic Press
eBook ISBN:
9780080860541
eBook ISBN:
9780080923987
Hardcover ISBN:
9780125215282
Paperback ISBN:
9780124023055

About the Serial Volume Editors

Stefan Kaufmann Serial Volume Editor

Affiliations and Expertise

Max Planck Institute for Infection Biology, Berlin, Germany

Dieter Kabelitz Serial Volume Editor

Affiliations and Expertise

University of Kiel, Germany