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Immunology & Serology in Laboratory Medicine
- 7th Edition - December 16, 2020
- Author: Mary Louise Turgeon
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 7 1 1 9 3 - 7
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 7 7 9 3 2 - 6
Building on a solid foundation of knowledge and skills, this classic text from trusted author Mary Louise Turgeon clearly explains everything from basic immunologic mechanisms an… Read more
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Request a sales quoteBuilding on a solid foundation of knowledge and skills, this classic text from trusted author Mary Louise Turgeon clearly explains everything from basic immunologic mechanisms and serologic concepts to the theory behind procedures performed in the lab. This go-to resource prepares you for everything from mastering automated techniques to understanding immunoassay instrumentation and disorders of infectious and immunologic origin. Packed with learning objectives, review questions, step-by-step procedures, and case studies, this text is the key to your success in today’s modern laboratory environment.
- Procedural protocols help you transition from immunology theory to practical aspects of the clinical lab.
- Case studies allow you to apply your knowledge to real-world situations and strengthen your critical thinking skills.
- Updated illustrations, photographs, and summary tables visually clarify key concepts and information.
- Full-color presentation clearly showcases diagrams and micrographs, giving you a sense of what you will encounter in the lab.
- Learning objectives and key terms at the beginning of each chapter provide measurable outcomes and a framework for organizing your study efforts.
- Review questions at the end of each chapter provide you with review and self-assessment opportunities.
- Cover image
- Title page
- Table of Contents
- Quick Reference
- Quick Reference—cont’d
- Copyright
- Dedication
- Reviewers
- Preface
- About the Author
- PART I. Basic Immunologic Mechanisms
- 1. Highlights of Innate and Adaptive Immune Systems
- What is Immunology?
- Cells of the Innate and Adaptive Immune Systems
- Origin and Development of Blood Cells
- Blood and Tissue Cells Associated With Innate and Adaptive Immune Systems
- Granulocytes
- Mononuclear Phagocyte System
- Monocytes
- Lymphocyte Variations
- Innate Lymphoid Cells
- Comparison of Innate and Adaptive Immunity
- 2. Soluble Mediators of the Immune System
- Complement System
- Classic Pathway
- Alternative Pathway
- Mannose-Binding Lectin Pathway
- Biological Functions of Complement Proteins
- Alterations in Complement Levels
- Diagnostic Evaluation
- Other Soluble Immune Response Mediators
- Hematopoietic Stimulators
- Acute-Phase Proteins
- 3. Antigens and Antibodies
- Antigen Characteristics
- Chemical Nature of Antigens
- Physical Nature of Antigens
- General Characteristics of Antibodies
- Immunoglobulin (Ig) Classes
- Antibody Structure
- Immunoglobulin Variants
- Antibody Synthesis
- Functions of Antibodies
- Antigen–Antibody Interaction: Specificity and Cross-Reactivity
- Molecular Basis of Antigen–Antibody Reactions
- Neutralizing Antibodies
- Monoclonal Antibodies
- 4. Cellular Activities and Clinical Disorders of Innate and Adaptive Immunity
- Innate Immunity
- Process of Phagocytosis
- Neutrophil Extracellular Traps
- Monocyte-Macrophage Host Defense Functions
- Cytokine Storm Syndromes
- Acute Inflammation
- Sepsis
- Cell Surface Receptors
- Disorders of Neutrophils
- Monocyte-Macrophage Disorders
- Disease States Involving Leukocyte Integrins
- Adaptive Immunity
- Lymphoid and Nonlymphoid Surface Membrane Markers
- Development of T Lymphocytes
- Innate Lymphoid Cells
- T Regulatory Lymphocytes
- B Lymphocytes
- Plasma Cell Biology
- Alterations in Lymphocyte Subsets
- Immunologic Disorders
- PART II. The Theory of Immunologic and Serologic Procedures
- 5. Basic Safety in the Immunology-Serology Laboratory
- Safety Standards and Agencies
- Safety Manual
- Patient Safety
- Prevention of Transmission of Infectious Diseases
- Safe Work Practices for Infection Control
- Protective Techniques for Infection Control
- Hand Sanitizing and Handwashing
- Other Safety Practices
- Laboratory Decontamination
- Disposal of Infectious Laboratory Waste
- Disease Prevention
- 6. Basic Quality Control and Quality Assurance Practices
- Clinical Laboratory Regulatory and Accrediting Organizations
- Nonanalytic Factors Related to Testing Accuracy
- Errors Related to Phase of Testing
- Quality Descriptors
- Monitoring Quality
- Reference Range Statistics
- Testing Outcomes
- Validating New Procedures
- 7. Basic Serologic Laboratory: Techniques and Clinical Applications
- Procedures Manual
- Blood Specimen Preparation
- Rapid Point-of-Care Testing
- 8. Precipitation and Particle Agglutination Methods
- Principles of Precipitation and Particle Agglutination Assays
- Latex Agglutination
- Pregnancy Testing
- Alternative Procedural Protocols
- Flocculation Tests
- Direct Bacterial Agglutination
- Hemagglutination
- Nephelometry
- 9. Electrophoresis Techniques and Chromatography
- Electrophoresis
- Serum Protein Electrophoresis
- Immunofixation Electrophoresis
- Capillary Electrophoresis
- Chromatography and Immunochromatography
- 10. Labeling Techniques in Immunoassay
- Immunoassay Formats
- Types of Labels
- Chemiluminescence
- Immunofluorescence
- Alternative Labeling Technologies
- 11. Flow Cytometry
- Characteristics of Instrumentation
- Flow Cell Cytometry
- 12. Molecular Laboratory Techniques
- Characteristics of Nucleic Acids
- Molecular Amplification Methods
- Molecular Analysis of Amplification Products
- Massively Parallel Sequencing/Next-Generation Sequencing Technology
- PART III. Immunologic Manifestations of Infectious Diseases
- 13. Infectious Diseases: Overview and TORCH Diseases
- Characteristics of Infectious Diseases
- Development of Infectious Diseases
- Traditional Infectious Disease Laboratory Testing
- Bacterial Diseases
- Parasitic Diseases
- Fungal Diseases
- Viral, Rickettsial, and Mycoplasmal Diseases
- COVID-19 (SARS-CoV-2 Virus)
- Dengue Fever
- TORCH Test Panel
- Toxoplasmosis
- Rubella
- Rubeola (Measles)
- Cytomegalovirus
- Herpes Viruses
- Alternate Immunology Laboratory Techniques
- 14. Streptococcal Infections
- Etiology
- Epidemiology
- Signs And Symptoms
- Diagnostic Evaluation
- Streptococcal Toxic Shock Syndrome
- Group B Streptococcal Disease
- 15. Syphilis
- Etiology
- Epidemiology
- Signs and Symptoms
- Primary Syphilis
- Secondary Syphilis
- Latent Syphilis
- Late (Tertiary) Syphilis
- Immunologic Manifestations
- Diagnostic Evaluation
- Direct Observation of Spirochetes
- Nontreponemal Methods
- Treponemal Methods
- Sensitivity of Representative Procedures for Syphilis
- Traditional Versus Reverse-Screening Algorithm Protocols
- 16. Vector-Borne Diseases
- Lyme Disease
- Human Ehrlichiosis
- Rocky Mountain Spotted Fever
- Babesiosis
- Chikungunya Disease
- Dengue Virus
- West Nile Virus
- Zika Virus
- 17. Infectious Mononucleosis
- Etiology
- Epidemiology
- Signs and Symptoms
- Laboratory Diagnostic Evaluation
- Immunologic Manifestations
- 18. Viral Hepatitis
- General Characteristics of Hepatitis
- Hepatitis A
- Hepatitis B
- Hepatitis D
- Hepatitis C
- Hepatitis E
- Hepatitis G
- Transfusion-Transmitted Virus
- PART IV. Immune Disorders
- 19. Primary and Acquired Immunodeficiency Syndromes
- Primary Immunodeficiency Disorders
- Characteristics of Primary Immunodeficiency Disorders
- General Laboratory Evaluation
- T Cell Immunodeficiency Disorders
- Digeorge Syndrome
- Severe Combined Immunodeficiency (SCID)
- Chronic Mucocutaneous Candidiasis
- Bruton X-Linked Agammaglobulinemia
- Common Variable Immunodeficiency
- Transient Hypogammaglobulinemia of Infancy
- Immunodeficiency with Elevated Immunoglobulin M (Hyper-IgM)
- Selective Immunoglobulin A Deficiency
- X-Linked Lymphoproliferative Disease (XLP) Syndromes 1 and 2 (Duncan Disease)
- Combined Cellular Immunodeficiency Disorders
- Partial Combined Immune Deficiency Disorders
- Other Primary Immune Disorders
- Hyper-E Syndrome (Job Syndrome)
- Acquired (Secondary) Immunodeficiency Disorders
- Acquired Immunodeficiency Syndrome
- Prevention
- Treatment
- Preexposure and Postexposure Prophylaxis
- 20. Hypersensitivity Reactions
- What is Hypersensitivity?
- What is an Allergy?
- Types of Antigens and Reactions
- Types of Hypersensitivity Reactions
- Comparison of Types of Hypersensitivity
- 21. Immunoproliferative Disorders
- General Characteristics of Gammopathies
- Multiple Myeloma
- Waldenström Primary Macroglobulinemia
- Other Monoclonal Disorders
- 22. Tolerance, Autoimmunity, and Autoimmune Disorders
- Immunologic Tolerance
- Factors Influencing Development of Autoimmunity
- Major Autoantibodies
- Autoimmune Disease
- Innate Immune System
- Adaptive Immune Response
- Comparison of Organ-Specific and Organ-Nonspecific Autoimmune Disorders
- Organ-Specific and Midspectrum Disorders
- 23. Systemic Lupus Erythematosus
- Different Forms of Lupus
- Etiology
- Epidemiology
- Signs and Symptoms
- Diagnostic Evaluation
- Treatment
- 24. Rheumatoid Arthritis
- Etiology
- Epidemiology
- Signs and Symptoms
- Anatomy and Physiology of Joints
- Immunologic Manifestations
- Diagnostic Evaluation
- Rheumatoid Factor
- Cyclic Citrullinated Peptide Antibodies
- Juvenile Idiopathic Arthritis
- Treatment
- Diagnostic Procedures
- PART V. Transplantation & Tumor Immunology
- 25. Transplantation: Human Leukocyte Antigens, Solid Organ, and Hematopoietic Stem Cells
- Histocompatibility Antigens
- Transplantation Terminology
- General Facts About Transplantation
- Pretransplantation Screening
- Types of Transplants
- Sources of Stem Cells for Transplantation
- Graft Rejection
- Mechanisms of Rejection
- Transplantation Complications
- Biomarkers for Rejection
- FOXP3 Messenger Ribonucleic Acid (MRNA)
- Graft-Versus-Host Disease
- Current Directions
- 26. Tumor Immunology and Applications of Massive Parallel Sequencing/Next-Generation Sequencing
- Cancer Stem Cells
- Types of Tumors
- Epidemiology
- Causative Factors in Human Cancer
- Impact of Somatic Mutations
- Stages of Carcinogenesis
- Cancer-Predisposing Genes
- Proto-Oncogenes
- Role of Oncogenes
- Body Defenses Against Cancer
- Tumor Markers
- DNA Microarray Technology
- What’s New in Cancer Diagnostic Testing?
- Modalities for Treating Cancer
- PART VI. Vaccines
- 27. Primer on Vaccines
- Goal of Vaccination
- What is a Vaccine?
- History of Vaccines
- Types of Vaccines
- Model of Vaccine Development
- Host Response to Vaccination
- Development of a Covid-19 Vaccine
- Rates of Vaccination
- Sites of Vaccine Administration
- Representative Diseases Associated With Newer Approved or Investigational Vaccines
- Dengue Fever Vaccine
- International Travel Vaccines
- Cancer Vaccines
- Vaccines in Biodefense
- Safety Issues
- Appendix A. Answers to Case Study Multiple Choice Questions
- Appendix B. Answers to Review Questions
- Appendix C. Origin and Immunoregulatory Activity of Cytokines
- Appendix D. Coronavirus Disease 2019 (COVID-19)
- Appendix E. Vaccines Licensed in the United States by the Federal Drug Administration
- Bibliography
- Glossary
- Index
- No. of pages: 584
- Language: English
- Edition: 7
- Published: December 16, 2020
- Imprint: Mosby
- Paperback ISBN: 9780323711937
- eBook ISBN: 9780323779326
MT
Mary Louise Turgeon
Mary Louise Turgeon, EdD, MLS(ASCP)CM, is an educator, author, and consultant in medical laboratory science education. Her career includes roles as a community college professor, Medical Laboratory Technology program director, undergraduate and graduate university professor, Medical Laboratory Science program director, and departmental chairperson. Dr. Turgeon is currently an ad hoc educational content specialist for the College of Professional Studies, Northeastern University, Boston,
and maintains an active clinical laboratory science consulting practice. Her practice, Mary L. Turgeon and Associates, focuses on new program development, curriculum revision, and increasing teaching effectiveness
through the use of technology and interactive teaching strategies.
Affiliations and expertise
Associate Professor (Adjunct), University of Texas Medical Branch, Galveston, Texas; Clinical Laboratory Education Consultant, Mary L. Turgeon and Associates, Boston, Massachusetts; St. Petersburg, Florida